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Ribonucleic acid chain polymerization amplification reaction detection device and method for detecting dna concentration

A detection device and ribonucleic acid technology, applied in chemical instruments and methods, biochemical cleaning devices, biochemical equipment and methods, etc., can solve problems affecting the reliability of Ct values, reduce peripheral equipment, improve efficiency, and facilitate The effect of batching

Active Publication Date: 2018-05-18
芜湖达辉生物科技有限公司
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Problems solved by technology

The limitations of this method are: 1. Exogenous standard samples or endogenous genes are required to standardize to draw a standard curve
2. Amplification caused by non-specific reaction will affect the reliability of Ct value

Method used

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  • Ribonucleic acid chain polymerization amplification reaction detection device and method for detecting dna concentration
  • Ribonucleic acid chain polymerization amplification reaction detection device and method for detecting dna concentration
  • Ribonucleic acid chain polymerization amplification reaction detection device and method for detecting dna concentration

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Embodiment Construction

[0037] With reference to the accompanying drawings, the specific implementation of the present invention will be further described in detail through the description of the embodiments to help those skilled in the art have a more complete, accurate and in-depth understanding of the inventive concept and technical solution of the present invention.

[0038] As mentioned earlier, the factors related to the success of the polymerization reaction include: 1. The limited dilution of the sample; 2. The total amount of the final product; 3. The Poisson distribution of the product amount and the initial DNA concentration. relationship. By adjusting and measuring the above factors, the starting DNA sample concentration can be calculated.

[0039] Based on this principle, the present invention provides figure 1 The structure shown is a micro-droplet flow type high-throughput ribonucleic acid chain polymerization amplification reaction detection device, which is widely used in the fields of mo...

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Abstract

The invention discloses a ribonucleic acid chain type polymerization amplification reaction detecting device and a detecting method thereof. The device is provided with a micro-droplet generating system driven by an ultrasonic vibration drive system, wherein a plurality of sampling holes are formed in the micro-droplet generating system; the ultrasonic vibration drive system generates vibration, so that a PCR sample in the micro-droplet generating system is mixed with oil drops to form droplets packaged with oil, and then, the droplets packaged with oil enter a polymerization reaction system. By adopting the technical scheme, generating of micro-droplets, polymerization reaction and reading of fluorescent data are carried out on the same equipment, so that sample loss is avoided, and therefore, the device is convenient, accurate and quick; requirements of a temperature gradient for in-vitro amplifying DNA can be effectively met, the biochemical reaction speed is remarkably increased, time is saved, efficiency is improved, and batch and low-cost production are convenient; PCR amplification circulating micro-fluidic bioluminescence is realized to feed back fluorescence information in real time; the device is more automatic and miniaturized, so that system working period is shortened; and missing of sample signal collection is avoided.

Description

Technical field [0001] The invention belongs to the technical field of bioengineering experimental instruments and equipment, and relates to PCR reagent amplification and fluorescence measurement and detection technologies. More specifically, the invention relates to a detection device integrated with data processing for ribonucleic acid chain polymerization amplification reactions. In addition, the invention also discloses the detection method of the detection device. Background technique [0002] PCR technology (DNA Polymerase Chain Reaction: DNA polymerase chain reaction) is an in vitro nucleic acid amplification technology developed in the mid-1980s. Khorana first proposed this idea in 1971. American scientist Kary Mullis and others realized this idea in 1985 and invented the epoch-making PCR technology. By applying this technology, the target gene to be studied can be amplified to 100,000 or even million times in a few hours in a test tube, which is convenient for direct ob...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/38C12M1/34C12M1/00
CPCB01L7/52B01L2200/143B01L2200/147B01L2300/1805C12Q1/686C12Q2563/159C12Q2523/301
Inventor 周辉王伟东
Owner 芜湖达辉生物科技有限公司
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