A kind of composite microbial profile control bacteria agent and its preparation method and application

A technology of compound microorganisms and microorganisms, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., to achieve the effects of simple process, safe construction and good injectability

Active Publication Date: 2020-01-07
PETROCHINA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a compound microbial profile control agent suitable for low-permeability non-fractured oil reservoirs and its preparation method to solve the deep profile control problem of low-permeability non-fractured oil reservoirs in an environmentally friendly way

Method used

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  • A kind of composite microbial profile control bacteria agent and its preparation method and application
  • A kind of composite microbial profile control bacteria agent and its preparation method and application
  • A kind of composite microbial profile control bacteria agent and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] First select the strain

[0040] Trichrodispora cocoa ( Lasiodiplodia theobromae ) There are differences among different strains, so the selection of strains is carried out first, and according to the general requirements of profile control agents, each Trichodispora cocoa ( Lasiodiplodia theobromae ) of different strains of bacteria ball diameter, polysaccharide metabolite content and fermentation broth viscosity were investigated.

[0041] Prepare PDA medium, the formula is: 1.0L of potato extract, 20.0g of glucose, inoculated with each Trichodiospora cocoa strain, and cultured in a shaker flask at 28°C and 120rpm for 3 days. After the cultivation, measure the average diameter of the bacterial spheres, centrifuge the fermentation broth at 8000rpm for 30 minutes, collect the upper layer liquid, and measure the viscosity of the fermentation liquid according to the GB / T10247-2008 rotation method; collect the lower layer sediment, add 6mol / L sodium hydroxide for leach...

Embodiment 2

[0062] The culture method of the microbial profile control bacterium agent in the above-mentioned embodiment, this method comprises the steps:

[0063] 1) Prepare PDA optimized medium;

[0064] 2) Put the optimized PDA medium prepared in step 1 into a sterilizer and sterilize at 120-121°C for 20-30 minutes. After the bacteria are finished, pour the culture medium into the petri dish while it is still hot to avoid solidification;

[0065] 3) The refrigerated Trichodispora cocoa ( Lasiodiplodia theobromae ) CFCC 85199 Take out and activate for 10 to 15 minutes, and use an inoculation needle to pick out the activated Trichodispora cacao for profile modification ( Lasiodiplodia theobromae ) CFCC 85199 surface hyphae, in step 2) the surface of the petri dish equipped with PDA plate medium is streaked and cultured, and cultured upside down in a constant temperature incubator at 28-30°C for 2-3 days;

[0066] On the PDA plate medium, where the temperature is 28-35°C and the rel...

Embodiment 3

[0073] The microbial profile-controlling agent in Example 1 was prepared into a composite microbial profile-controlling agent, which was composed as follows in terms of mass percentage: microbial profile-controlling agent 5-10%; corn grain 0.2-0.3%; polymer 0.2-0.5% %; additives 0-0.2%; the balance of water.

[0074] The microbial profile-controlling agent is rich in mycospheres and polysaccharide metabolites naturally formed during the growth and reproduction of microorganisms. It is the main substance of the composite microbial profile-controlling agent and exerts the main profile-regulating effect.

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Abstract

The invention provides a compound microorganism profile control bacterium agent as well as a preparation method and application thereof. The compound microorganism profile control bacterium agent is prepared from the following components in percentage by mass: 5 percent to 10 percent of a microorganism profile control bacterium agent, 0.2 percent to 0.3 percent of corn grains, 0.2 percent to 0.5 percent of a high molecular polymer, 0 to 0.2 percent of an auxiliary agent and the balance of water, wherein the microorganism profile control bacterium agent is fermentation liquid obtained by fermentation culture on lasiodiplodia theobromae for profile control with the preservation register number of CFCC 85199 in an optimized PDA culture medium; the compound microorganism profile control bacterium agent contains rich mycelial fungi and polysaccharide metabolites which are naturally formed in a microorganism growing and propagating process; when being applied to a low-permeation non-crack oil reservoir, the compound microorganism profile control bacterium agent can enter a deep part of the low-permeation oil reservoir under a suspension effect of the high molecular polymer; growth and propagation are carried out by utilizing a slow-release nutrient component, namely the corn grains, and the auxiliary agent, or the corn grains are used as carriers for growth in an attachment manner; pore permeation type high-permeation belts, micro-cracks and the like are effectively blocked; the compound microorganism profile control bacterium agent is low in injection viscosity, good in injection property, simple in process and safe to construct.

Description

technical field [0001] The invention relates to the field of microbial profile-controlling agents, in particular to a composite microbial profile-controlling agent and a preparation method thereof, and the application of the modified composite microbial profile-controlling agent in low-permeability non-fractured oil reservoirs. Background technique [0002] Due to the long-term water injection development of the oilfield, large pores and high permeability zones are formed in the reservoir, and problems such as rising water cut in oil wells and decreasing oil production rate appear. [0003] At present, the water plugging profile control technology of using profile control agent to plug the high aquifer layer of the oil reservoir can improve the heterogeneity of the formation, increase the swept volume of water flooding, and improve the efficiency of crude oil recovery. It has become an application in tertiary oil recovery One of the broadest and most conventional means. How...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C09K8/50C12R1/645
CPCC09K8/50C12N1/14
Inventor 李忠兴赵继勇李兆国李文宏张永强向中远徐飞艳张宏强范伟陈富林薛姝雯
Owner PETROCHINA CO LTD
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