Fluorescence detection method for time resolution fluorescence marker and device of method

Abstract

The invention provides a fluorescence detection method for a time resolution fluorescence marker and a device of the method. A sample solution to be detected is divided into a plurality parts with the equal amount, the fluorescence marker is detected in batches to obtain statistic results obtained in multiple times of detection, and then detection on the content of the fluorescence marker in a whole sample solution is completed, wherein the sample solution to be detected contains magnetic microspheres coated with the time resolution fluorescence marker. Accordingly, the detection accuracy of a reaction tank is improved, and system detection fluctuation is reduced.

Description

technical field [0001] The invention belongs to the technical field of medical detection, and in particular relates to a fluorescence detection method of a time-resolved fluorescent marker and a device thereof. Background technique [0002] With the advancement of clinical medicine and technology, fluorescence immunoassay technology has developed a variety of practical detection technologies, such as electrochemical fluorescence immunoassay (ECLI), chemiluminescence immunoassay (CLIA), time-resolved fluorescence immunoassay (TRFIA) and other fluorescence immunoassay techniques . These technologies all determine the detected substance by detecting the marker (tracer probe). The marker is made into a A probe with high specificity, the probe binds to the target detection substance through a corresponding reaction; then the tracer probe is detected by a fluorescence instrument to obtain relevant information such as the concentration of the target substance. On the other hand, ...

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Problems solved by technology

[0004] 1. The detection magnetic microspheres are distributed in the sample solution at a low concentration, which is easy to cause deviations in the detection results due to uneven distribution of magnetic microspheres or incomplete cleaning;

[0005] 2. Although the sampling of magnetic microspheres is accurate, the remaining number of magnetic microspheres in the reaction pool cannot be determined after repeated cleaning

[0007] With the development of laboratory medicine, there will be more and more determinations of trace and ultra-trace quantities. Time-resolved fluorescence analysis (TRFIA) has more and more application space, but there is no fully automatic time-resolved fluorescence analysis method on the market. detection system (i.e. stochastic system)

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