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Biomarkers VWF and ADAMTS13 and use thereof in liver cirrhosis diagnostic reagents

一种诊断试剂、用途的技术，应用在疾病生物标志物用途领域，能够解决患者无法进行FibroScan检查等问题，达到安全无创伤易于、特异性和灵敏性高、操作简单易行的效果

Active Publication Date: 2016-11-02
CHONGQING ZAOQITIAN BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A considerable number of patients cannot be examined by FibroScan

Method used

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  • Biomarkers VWF and ADAMTS13 and use thereof in liver cirrhosis diagnostic reagents
  • Biomarkers VWF and ADAMTS13 and use thereof in liver cirrhosis diagnostic reagents
  • Biomarkers VWF and ADAMTS13 and use thereof in liver cirrhosis diagnostic reagents

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Example 1: Preparation of gold label kit and its application in the diagnosis of liver cirrhosis.

[0061] 1. Preparation of the kit:

[0062] The antibody used in the preparation process of this kit is the specific antibody of VWF or ADAMTS13. Note, during the preparation of the same kit: if the monoclonal antibody 1 used in the gold-labeled antibody glass fiber membrane is an antibody of VWF, then the monoclonal antibody 2 and polyclonal antibody used in the preparation of the nitrocellulose-coated membrane should also use VWF Antibody; if the monoclonal antibody 1 used in the gold-labeled antibody glass fiber membrane is the antibody of ADAMTS13, then the monoclonal antibody 2 and polyclonal antibody used in the preparation of the nitrocellulose-coated membrane should also use the antibody of ADAMTS13.

[0063] 1. Preparation of colloidal gold solution: put ultrapure water on a magnetic stirrer and stir and heat to boiling, then quickly add chloroauric acid solution...

Embodiment 2

[0084] Example 2: Sensitivity and specificity studies of VWF and ADAMTS13 as biomarkers of liver cirrhosis.

[0085] To define sensitivity and specificity as described here:

[0086] Sensitivity: The sensitivity stated in this article is the proportion of patients with chronic liver disease who actually have cirrhosis who are correctly judged as true positives; or the proportion of patients who actually enter the decompensated stage of liver cirrhosis as true positives.

[0087] Specificity: The specificity described in this article is the proportion of patients with chronic liver disease who do not actually suffer from cirrhosis who are correctly judged as true negative; or the proportion of patients who are actually in compensated cirrhosis who are correctly judged as true negative.

[0088] VWF and ADAMTS13 liver cirrhosis ratio critical value: a ratio data of VWF and ADAMTS13 content specified in this article, if the ratio of VWF and ADAMTS13 content in the subject’s serum...

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PUM

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Abstract

Relating to the field of use of disease biomarkers, the invention discloses liver cirrhosis biomarkers VWF (von willebrand factor) and ADAMTS13 (adisintegrin and metalloproteinase with thrombospondin motifs 13) and use thereof in liver cirrhosis diagnostic reagents. The VWF is composed of protein with an amino acid sequence accession number of GI 317373549 in a NCBI database, and the ADAMTS13 is composed of protein with an amino acid sequence accession number of GI 74749836 in a NCBI database. Use of the biomarkers VWF and ADAMTS13 for liver cirrhosis diagnosis on subjects has the advantages of simplicity and feasibility, safe and non-invasive diagnostic process and easy acceptance by patients, unified diagnosis standard insusceptible to subjective factors, high diagnosis result specificity and sensitivity, and easy mass screening and selection, etc.

Description

technical field [0001] The invention relates to the field of application of disease biomarkers, in particular to liver cirrhosis biomarkers VWF and ADAMTS13 and their use in liver cirrhosis diagnostic reagents. Background technique [0002] Liver cirrhosis is a chronic progressive liver disease with diffuse liver damage caused by long-term or repeated effects of one or more causes. In my country, most of them are post-hepatitis cirrhosis, and a small part are alcoholic cirrhosis and schistosome cirrhosis. Histopathologically, extensive liver cell necrosis, nodular regeneration of residual liver cells, connective tissue hyperplasia, and fibrous septum formation lead to structural destruction of hepatic lobules and formation of pseudolobules. The liver gradually deforms and hardens and develops into cirrhosis. In the early stage, there are no obvious symptoms due to the strong compensatory function of the liver. In the later stage, liver function damage and portal hypertensio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/543G01N33/558
CPCG01N33/543G01N33/558G01N33/6812G01N33/6893G01N2800/085
Inventor 高平
Owner CHONGQING ZAOQITIAN BIOTECHNOLOGY CO LTD
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