multifunctional cell culture flask

Abstract

The invention relates to a multifunctional cell culture bottle. The multifunctional cell culture bottle comprises a bottle body, a bottle neck and a propping-against support, wherein the bottle neck is inclined towards one side of a front side wall; a bottle opening at the upper end of the bottle neck is inclined upwards; the propping-against support is formed by successively splicing a plurality of secondary supports distributed vertically in the transverse direction; the adjacent secondary supports are matched with one another through U-shaped connecting sleeves and fastening bolts and are connected retractably; each secondary support comprises a supporting plate distributed vertically, a stand column, a moving sleeve and a U-shaped rotation connecting seat; two sides of each supporting plate respectively extend outwards to form retractable connecting parts; each stand column is perpendicularly fixed on the top surface of the corresponding supporting plate; each moving sleeve capable of moving vertically sleeves the corresponding stand column and is in threaded connection with a positioning bolt; each rotation connecting seat is fixedly connected to the corresponding moving sleeve; the adjacent retractable connecting parts of each two adjacent secondary supports are placed in a cavity of the same corresponding U-shaped connecting sleeve; a rotation connecting block is fixed on a back side wall of the bottle body, and can be connected to the inside of the rotation connecting seat in a vertical rotating manner. The multifunctional cell culture bottle is convenient to operate and diversified in function.

Description

technical field [0001] The invention relates to the technical field of cell culture bottles, in particular to multifunctional cell culture bottles. Background technique [0002] In the process of cell culture, rectangular parallelepiped flasks are widely used at present. The digestion and passage of cells are operated in ultra-clean benches or biological safety cabinets according to requirements. These two operating platforms are local purification for experimenters to operate. The equipment, through the circulating air flow, achieves local ultra-low sedimentation bacteria, creates a local high-clean air environment, and greatly reduces the probability of bacterial contamination during cell culture. It is a necessary special equipment for scientific research, pharmaceuticals, and medical and health operations. [0003] According to the characteristics of different types of cells, cell culture can be roughly divided into adherent cell culture and suspension cell culture. Dur...

AI Technical Summary

Problems solved by technology

This step needs to be performed in an ultra-clean bench. The experimenter needs a hand-held culture bottle. After opening the bottle cap, use a pipette or a pipette gun to add trypsin. During this period, the pipette needs to be changed or the trypsin container needs to be opened. , often requires two-handed operation, and the culture bottle is often placed on the table vertically with the open mouth, but the airflow in the ultra-clean bench is vertical from top to bottom, while the oblique opening of the culture bottle is upward, so there is a certain probability that it will be brought by the airflow Bacterial contamination caused by the outside or the operator's hand

In the next step of cell subculture after the adherent cells are suspended or the suspension cells are subcultured, the cells in the original culture flask need to be inoculated into several new culture flasks respectively. During the process of transferring cells out of the cell culture bottle, the other culture bottle can only be placed vertically on the table, and the movement of the operator's hand in the ultra-clean table disturbs the vertical air flow, causing bacteria to easily enter the bottle mouth and increase the Chance of infection

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