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Polypeptides that can be modified by o-glycosylation

An amino acid and glycoprotein technology, applied in the direction of peptides, hybrid peptides, specific peptides, etc., can solve the problems of single sequence, which is not conducive to the immune process such as antigen presentation and recognition.

Active Publication Date: 2021-11-30
INST OF BIOENG ACAD OF MILITARY MEDICAL SCI OF THE CHINESE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the long sequence of the glycosylation site, the peptide sequence in the finally recognized sugar-peptide is single, which is not conducive to the immune process such as antigen presentation and recognition.

Method used

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  • Polypeptides that can be modified by o-glycosylation
  • Polypeptides that can be modified by o-glycosylation
  • Polypeptides that can be modified by o-glycosylation

Examples

Experimental program
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Embodiment 1

[0144] Example 1, O-glycosylation of polypeptides

[0145]The polypeptides provided in this example are the polypeptides of a1) and a2): a1) the polypeptide whose sequence is W-P-Xn-S-Ym (ie, the core peptide); a2) the polypeptide containing the sequence described in a1); wherein, Xn is Polypeptides of 1 or 2 or 3 or 4 amino acids, Ym of 1 or 2 or 3 amino acids, a1) and a2) can all be O-glycosylated. The amino acid sequence of Xn is any one of the following A1)-A12): A1) A-A-A; A2) A-A; A3) A; A4) G-N-N-T; A5) A-N-N-T; A6) G-A-N-T; A7) G-N-A-T; A8) G-N-N-A; A9) G-A-T; A10) G-T; A11) G-N-T; A12) G-A-A; the amino acid sequence of Ym is any one of the following B1)-B25): B1) A-G-V; B2) A-P; B3) A-G; B4) G-P; B5) M-P; B6) S-P; B7) Q-P; B8) F-P; B9) V-P; B10) L-P; B11) I-P; B12) P-P; B13) N-P; B14) T-P; B15) C-P; B16) A-A-V; A-G-A; B18) A; B19) W-P; B20) Y-P; B21) D-P; B22) E-P; B23) K-P; B24) R-P; B25) H-P.

[0146] The polypeptide of a1) is specifically a polypeptide whose ami...

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Abstract

The present invention discloses polypeptides that can be modified by O-glycosylation. The polypeptide provided by the present invention is: a1) a polypeptide whose sequence is W-P-Xn-S-Ym; a2) a polypeptide containing the sequence described in a1); wherein, Xn is 1 or 2 or 3 or 4 amino acids, Ym It is 1 or 2 or 3 amino acids; the amino acid in the amino acid is any one of 20 kinds of natural amino acids. Experiments have proved that the polypeptides that can be O-glycosylated provided by the present invention can only include W-P-Xn-S-Ym polypeptides (ie core peptides), the sequence has reached the shortest, and can be inserted into the surface of the protein The site with strong immunogenicity is beneficial to the immune process such as antigen presentation and recognition by MHC-II. The polypeptide that can be O-glycosylated provided by the present invention can be used to produce polysaccharide-conjugated vaccines by biological methods.

Description

technical field [0001] The present invention relates to polypeptides that can be modified by O-glycosylation in the field of biomedicine. Background technique [0002] For a long time, it was believed that protein glycosylation was only present in eukaryotes. Since Mescher first discovered the existence of glycoproteins and glycosylation modifications in bacteria in 1974, people have found the existence of protein glycosylation in all life fields such as prokaryotes, eukaryotes, and archaea. [0003] Protein glycosylation includes O-glycosylation and N-glycosylation, mostly O-glycosylation in bacteria. Neisseria meningitidis glycosyltransferase PglL is an O-oligosaccharide transferase, which can catalyze O-glycosylation of Neisseria meningitidis type IV pilus protein PilE when present alone. At the same time, because the glycosylation process is very similar to the synthesis of bacterial lipopolysaccharide LPS, it is possible to use bacterial glycosylation to produce polys...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/06C12N15/11C12N15/63C12N1/15C12N1/19C12N1/21C07K19/00A61K39/385A61K39/02A61P31/04
Inventor 王恒樑潘超朱力吴军冯尔玲刘先凯孙鹏王东澍曾明王斌
Owner INST OF BIOENG ACAD OF MILITARY MEDICAL SCI OF THE CHINESE