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Method and system for processing immune repertoire sequencing data of samples

A technology of sequencing data and immune repertoire, applied in the field of immune repertoire, can solve problems that need to be improved, and achieve the effects of high accuracy, good repeatability and wide applicability

Active Publication Date: 2016-11-23
BGI SHENZHEN CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in view of such a complex genetic structure and large amount of data, accurate information analysis methods are also a challenge
[0003] Therefore, the current immune repertoire research methods still need to be improved

Method used

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  • Method and system for processing immune repertoire sequencing data of samples
  • Method and system for processing immune repertoire sequencing data of samples
  • Method and system for processing immune repertoire sequencing data of samples

Examples

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Embodiment 1

[0118] Peripheral blood was collected from two healthy individuals to obtain two peripheral blood samples (sample 1 and sample 2), wherein sample 1 was used to measure TRB and sample 2 was used to measure IGH. Peripheral blood samples were amplified by multiplex PCR to construct an immune panel, and then sequenced with Illumina's Paired-end 100 (sample 1) and PE150 (sample 2) (http: / / www.illumina.com / ), to obtain Sequencing data of immune repertoires for two samples. Specifically: multiple pairs of primers are designed in the V and J regions of TRB / IGH, so that all functional rearranged genes can be captured through multiplex PCR amplification; DNA is extracted from peripheral blood, and multiplex PCR is performed together with the primers designed above Reaction, PCR for 25 cycles; electrophoresis of multiple PCR products, recovery of 100-200bp (for TRB samples) fragments, and 100bp-300bp (for IGH samples) fragments, and then end repair and end addition "A", then connect the...

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Abstract

The invention discloses a method and system for processing immune repertoire sequencing data of samples. The method includes the steps that 1, data filtering processing is carried out on the immune repertoire sequencing data of the samples; 2, sequence splicing processing is carried out on the sequencing data obtained after data filtering processing; 3, local comparison is carried out on the sequencing data obtained after sequence splicing processing; 4, re-comparison is carried out on local comparison results; 5, the result with the highest score is screened out of the re-comparison results, and filtering is carried out; 6, error correction processing is carried out on the final comparison result; 7, sequence structure determination and translation are carried out based on the final comparison result obtained after error correction processing. By means of the method, analysis of a large quantity of immune repertoire sequencing data can be effectively achieved, analysis of TCR data and analysis of BCR data can be achieved at the same time, the PCR and sequencing errors can be effectively handled, the accuracy is high, the repeatability is good, and thus the whole condition of an immune repertoire can be truly and effectively reflected.

Description

technical field [0001] The present invention relates to the technical field of immune repertoire, in particular to a method and system for processing sequencing data of a sample immune repertoire. Background technique [0002] The immune repertoire (immune reperoire) refers to the collection of B cell receptors / antibodies (BCR) or T cell receptors (TCR) that constitute the body's immune system. B-cell receptors / antibodies are composed of two heavy chains and two light chains, and T-cell receptors are composed of α-chain β-chain or γ-chain δ-chain (rarely, ~5%). During the process of lymphocyte maturation, Each chain is produced by the rearrangement of multiple types of genes (heavy chain, β chain and δ chain are rearranged by V, D, J, C genes, light chain, α chain and γ chain are rearranged by V, J, C genes) , where there are many genes of each type, so that there are thousands to tens of thousands of different types of gene combinations in each chain. In addition, when di...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G06F19/10
Inventor 张伟刘晓杜元平王长希
Owner BGI SHENZHEN CO LTD
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