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CHO-S cell semisolid medium as well as preparation method and application thereof
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A semi-solid medium, CHO-S technology, applied in the direction of artificial cell constructs, animal cells, vertebrate cells, etc., can solve problems that are not known to the public, and achieve reduced dependence, good growth status, and feasibility strong effect
Active Publication Date: 2016-12-07
SHANGHAI JMT BIO INC
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The formula and preparation method of CloneMedia-CHO-G semi-solid medium are trade secrets of Mocular Devices and are not known to the public
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Embodiment 1
[0042] Embodiment 1, CHO-S cell semi-solid medium formula
[0043] Material:
[0044] CD FortiCHO AGT Medium Prototype, supplier Gibco, Cat. No. A14286EF;
[0045] Methylcellulose, supplied by Sigma, product number M0262-500G, average molecular weight 41000, at 20°C, 2% of this methylcellulose has a viscosity of 400cPs.
[0046] In weight percent: 2.46% CD FortiCHO AGT Medium Prototype, 2% methylcellulose.
Embodiment 2
[0047] Embodiment 2, CHO-S cell semi-solid medium formula
[0048] CD FortiCHO AGT Medium Prototype, supplier Gibco, Cat. No. A14286EF;
[0049] Methylcellulose, supplied by Sigma, product number M0262-500G, average molecular weight 41000, at 20°C, 2% of this methylcellulose has a viscosity of 400cPs.
[0050] In weight percent: 2.46% CD FortiCHO AGT Medium Prototype, 3% methylcellulose.
Embodiment 3
[0051] Embodiment 3, CHO-S cell semi-solid medium formula
[0052] CD FortiCHO AGT Medium Prototype, supplier Gibco, Cat. No. A14286EF;
[0053] Methylcellulose, supplied by Sigma, product number M0262-500G, average molecular weight 41000, at 20°C, 2% of this methylcellulose has a viscosity of 400cPs.
[0054] In weight percent: 2.46% CD FortiCHO AGT Medium Prototype, 2.7% methylcellulose.
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Abstract
The invention discloses a CHO-S cell semisolid medium as well as a preparation method and an application thereof. The semisolid medium consists of the following components in percentage by weight: 2.46-2.48% of a CD FortiCHO medium and 2-3% of methyl cellulose. The CHO-S cell semisolid medium is prepared by the following steps: fully uniformly mixing the 2*CD FortiCHO and the 2*methyl cellulose according to a volume ratio of 1 to 1, conducting centrifuging so as to remove particles which are invisible to naked eyes, and collecting upper centrifugal liquid. The CHO-S cell semisolid medium disclosed by the invention can be used for replacing an imported CHO-S cell semisolid medium in cultivating CHO-S cells; cells are great in growth, high in protein expression quantity and good in growth status; and research and development cost and production cost can be greatly reduced.
Description
technical field [0001] The invention relates to a semi-solid medium suitable for CHO-S cell growth and its preparation method and application, belonging to the field of CHO-S cell strain development. Background technique [0002] The targeting specificity and high expression of antibodies are of great significance in clinical practice, so it is particularly important to find cell lines that can stably secrete the correct antibodies in large quantities faster than competitors. In this regard, the traditional cell screening adopts the limited dilution method or cell sorting method, which is a lot of work and time-consuming. Usually, it takes 8 weeks to screen 1000 cells, and finally a good clone can be obtained; Causes mechanical damage, resulting in a decrease in the survival rate of clones. Now companies often use the semi-solid medium method for monoclonal screening. Cells grow in a semi-solid medium to form a monoclonalcell cluster, and at the same time secrete antibodi...
Claims
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Application Information
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