A preprocessing method for tissue sections that fail conventional fish detection

Abstract

The invention relates to a pretreatment method aiming to tissue section being failed in regular FISH detection, and particularly, provides four sodium citrate buffer solutions that are in different concentrations and pH values, a method of treating the tissue section being failed in the regular FISH detection with the sodium citrate buffer solutions, and a kit comprising the sodium citrate buffer solutions. A test proves that by means of the sodium citrate buffer solutions for pre-treating the tissue section being failed in the regular FISH detection, successful rate of the detection is increased to 71-73%. The pretreatment method is high in detection successful rate, has low cost and is suitable for being promoted.

Description

technical field [0001] The invention relates to the technical field of biological detection and molecular diagnosis, in particular to a pretreatment method for tissue sections that fail conventional FISH detection. Background technique [0002] FISH is the abbreviation of fluorescence in situ hybridization in English, which refers to a molecular biological method for qualitative, localization and relative quantitative research on nucleic acid on tissue sections, cell smears and chromosome preparations. , unique, intuitive and other advantages. The principle is that under the "quenching" condition after high temperature denaturation, the fluorescein-labeled nucleic acid probe sequence can specifically combine with the homologous and complementary target nucleic acid sequence in the target cell in situ. This technology and the commonly used PCR and nucleic acid sequencing technologies in clinical testing belong to the category of nucleic acid molecular pathological diagnosis,...

AI Technical Summary

Problems solved by technology

[0006] 2) Sodium thiocyanate treatment: It has been reported that compared with the citrate buffer heat treatment, the sodium thiocyanide treatment method significantly increases the chance of stripping, especially in the slices of the microtissue matrix (Tissue Microarray) At the same time, sodium thiocyanide has certain chemical toxicity. According to the interactive encyclopedia, it is a product with high environmental risk: it is easy to deliquesce in the air, and it will produce toxic gas when it encounters acid. Chronic poisoning will cause thyroid damage. The maximum allowable concentration in the air is 50mg / m 3 , and sodium thiocyanate is relatively expensive

[0011] 2) For patients with tumor recurrence, it is necessary to trace back and compare with the primary tumor or the original postoperative tissue block, and it may be encountered that the paraffin-embedded tissue block has been stored for more than 8 years or even longer;

[0014] 5) For some tissues, the signal of FISH labeling is still weak despite the improvement of other factors affecting the signal

[0015] In clinical diagnosis, when the above problems occur, after an additional 1-2 attempts to change the protease digestion time and change the elution intensity and other conditions, if the signal still does not meet the technical requirements of the diagnosis, the diagnosis is technically "failed" "The conclusion is acceptable, but it hinders clinical diagnosis and related scientific research.

Images