Kit for rapidly and quantitatively detecting troponin and creatine kinase isozyme

A troponin, quantitative detection technology, applied in the field of kits, can solve the problems of long detection time and low sensitivity

Active Publication Date: 2016-12-07
ANHUI IPROCOM BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In order to solve the technical problems of low sensitivity and too long detection time of the troponin and creatine kinase isoenzyme detection kits in the prior art, the purpose of the present invention is to provid

Method used

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  • Kit for rapidly and quantitatively detecting troponin and creatine kinase isozyme

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Example 1, a kit for rapid quantitative detection of troponin and creatine kinase isozymes

[0062] The kit described in Example 1 of the present invention includes a base plate (1), and the base plate is provided with sequentially overlapped sample pads (2), antibody binding pad I (3), antibody binding pad II (4), coated with Coated analytical membrane (5) and water-absorbent pad (6) of two detection lines and one quality control line, wherein the antibody-binding pad I is coated with biotin-labeled anti-troponin I monoclonal antibody The immunomagnetic bead I, the antibody binding pad II is coated with the immunomagnetic bead II coupled with the anti-creatine kinase isoenzyme monoclonal antibody, the surface of the magnetic bead I is coated with streptavidin, The two detection lines are detection line I (501) coated with troponin I monoclonal antibody and detection line II (501) coated with creatine kinase isoenzyme monoclonal antibody, and the quality control line (5...

Embodiment 2

[0086] Example 2, a kit for rapid quantitative detection of troponin and creatine kinase isozymes

[0087] The kit described in Example 2 of the present invention includes a base plate, and the base plate is provided with sequentially overlapped sample pads, antibody binding pad I, antibody binding pad II, and a coating coated with two detection lines and a quality control line. Analytical membrane and water-absorbing pad, wherein the antibody-binding pad I is coated with immunomagnetic beads I coupled with biotin-labeled anti-troponin I monoclonal antibody, and the antibody-binding pad II is coated with anti-troponin I monoclonal antibody Acid kinase isoenzyme monoclonal antibody-coupled immunomagnetic beads II, the surface of the magnetic beads I is coated with streptavidin, and the two detection lines are the detection of the coated troponin I monoclonal antibody Line I and detection line II coated with creatine kinase isoenzyme monoclonal antibody, the quality control line...

Embodiment 3

[0089] Example 3, a kit for rapid quantitative detection of troponin and creatine kinase isozymes

[0090] The kit described in Example 3 of the present invention includes a bottom plate, and the bottom plate is provided with sequentially overlapped sample pads, antibody binding pad I, antibody binding pad II, and a coating coated with two detection lines and a quality control line. Analytical membrane and water-absorbing pad, wherein the antibody-binding pad I is coated with immunomagnetic beads I coupled with biotin-labeled anti-troponin I monoclonal antibody, and the antibody-binding pad II is coated with anti-troponin I monoclonal antibody Acid kinase isoenzyme monoclonal antibody-coupled immunomagnetic beads II, the surface of the magnetic beads I is coated with streptavidin, and the two detection lines are the detection of the coated troponin I monoclonal antibody Line I and detection line II coated with creatine kinase isoenzyme monoclonal antibody, the quality control ...

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PUM

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Abstract

The invention belongs to the field of kits, and particularly relates to a kit for rapidly and quantitatively detecting troponin and creatine kinase isozyme, and a preparation method of the kit. The kit comprises a base plate, wherein a sample pad, an antibody binding pad I, an antibody binding pad II, an enveloped analysis film and a water absorption pad which are sequentially lapped are arranged on the base plate; an immunomagnetic bead which is coupled with biontin-marked anti-troponin I monoclonal antibody is enveloped on the antibody binding pad I, an immunomagnetic bead which is coupled with an anti-creatine kinase isozyme monoclonal antibody is enveloped on the antibody binding pad II, and the surface of the magnetic bead I is enveloped with streptavidin. The kit has the advantages of being simple and convenient to operate, fast to react, high in sensitivity, strong in specificity, suitable for on-site detection and the like, and is suitable for large-range popularization and application.

Description

technical field [0001] The invention belongs to the field of kits, in particular to a kit for rapid quantitative detection of troponin and creatine kinase isozymes. Background technique [0002] Cardiovascular disease is a great threat to human health and life, and has become one of the two major difficulties in medicine. Cardiovascular disease is the "number one killer" of human death in the world. There are nearly 200 million patients with cardiovascular disease in my country. Nearly 3 million people died of cardiovascular disease. Therefore, it is imminent to establish a detection system that can accurately, quickly and conveniently predict and diagnose cancer and cardiovascular diseases. [0003] Cardiovascular markers mainly include troponin I, troponin T, creatine kinase (CK) and creatine kinase isoenzyme (CK-MB), myoglobin, D-dimer, C-reactive protein, etc. [0004] Cardiac Troponin I (cTnI for short) is a specific and sensitive marker of myocardial cell death, which...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/573G01N33/543
CPCG01N33/54326G01N33/573G01N33/68G01N2800/32
Inventor 陈立国
Owner ANHUI IPROCOM BIOTECH CO LTD
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