Kit for rapidly and quantitatively detecting troponin and creatine kinase isozyme
A troponin, quantitative detection technology, applied in the field of kits, can solve the problems of long detection time and low sensitivity
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Embodiment 1
[0061] Example 1, a kit for rapid quantitative detection of troponin and creatine kinase isozymes
[0062] The kit described in Example 1 of the present invention includes a base plate (1), and the base plate is provided with sequentially overlapped sample pads (2), antibody binding pad I (3), antibody binding pad II (4), coated with Coated analytical membrane (5) and water-absorbent pad (6) of two detection lines and one quality control line, wherein the antibody-binding pad I is coated with biotin-labeled anti-troponin I monoclonal antibody The immunomagnetic bead I, the antibody binding pad II is coated with the immunomagnetic bead II coupled with the anti-creatine kinase isoenzyme monoclonal antibody, the surface of the magnetic bead I is coated with streptavidin, The two detection lines are detection line I (501) coated with troponin I monoclonal antibody and detection line II (501) coated with creatine kinase isoenzyme monoclonal antibody, and the quality control line (5...
Embodiment 2
[0086] Example 2, a kit for rapid quantitative detection of troponin and creatine kinase isozymes
[0087] The kit described in Example 2 of the present invention includes a base plate, and the base plate is provided with sequentially overlapped sample pads, antibody binding pad I, antibody binding pad II, and a coating coated with two detection lines and a quality control line. Analytical membrane and water-absorbing pad, wherein the antibody-binding pad I is coated with immunomagnetic beads I coupled with biotin-labeled anti-troponin I monoclonal antibody, and the antibody-binding pad II is coated with anti-troponin I monoclonal antibody Acid kinase isoenzyme monoclonal antibody-coupled immunomagnetic beads II, the surface of the magnetic beads I is coated with streptavidin, and the two detection lines are the detection of the coated troponin I monoclonal antibody Line I and detection line II coated with creatine kinase isoenzyme monoclonal antibody, the quality control line...
Embodiment 3
[0089] Example 3, a kit for rapid quantitative detection of troponin and creatine kinase isozymes
[0090] The kit described in Example 3 of the present invention includes a bottom plate, and the bottom plate is provided with sequentially overlapped sample pads, antibody binding pad I, antibody binding pad II, and a coating coated with two detection lines and a quality control line. Analytical membrane and water-absorbing pad, wherein the antibody-binding pad I is coated with immunomagnetic beads I coupled with biotin-labeled anti-troponin I monoclonal antibody, and the antibody-binding pad II is coated with anti-troponin I monoclonal antibody Acid kinase isoenzyme monoclonal antibody-coupled immunomagnetic beads II, the surface of the magnetic beads I is coated with streptavidin, and the two detection lines are the detection of the coated troponin I monoclonal antibody Line I and detection line II coated with creatine kinase isoenzyme monoclonal antibody, the quality control ...
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