A hyperbolic calibration quantitative immunochromatographic detection method
An immunochromatographic detection, calibration and quantification technology, which is applied in the field of hyperbolic calibration and quantitative immunochromatography detection, can solve the problems of complex process and uncertain dilution ratio, and achieve the effect of saving operation and improving detection efficiency.
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Embodiment 1
[0064] C-reactive protein hyperbolic calibration quantification
[0065] The pure C-reactive protein standard was serially diluted to obtain the following concentrations:
[0066] 0mg / L, 0.5mg / L, 1mg / L, 2.5mg / L, 10mg / L, 25mg / L, 50mg / L, 100mg / L, 110mg / L, 110mg / L, 125mg / L, 150mg / L, 175mg / L, 200mg / L.
[0067] The concentration of C-reactive protein to be tested is unknown.
[0068] Take equal amounts of different concentrations of C-reactive protein and the C-reactive protein to be tested and drop them on the test strips respectively.
[0069] The test strip is provided with a binding pad, a detection line and a control line.
[0070] The binding pad is coated with a first antibody labeled with a fluorescent marker and capable of binding to C-reactive protein;
[0071] The detection line is coated with a second antibody immobilized on the detection line which is different from the antigen epitope bound by the first antibody;
[0072] The control thread is coated with C-react...
Embodiment 2
[0100] Hyperbolic Calibration Quantification of Hepatitis B Surface Antibody
[0101] The pure hepatitis B surface antibody standard version was serially diluted to obtain the following concentrations:
[0102] 0mIU / ml, 50mIU / ml, 100mIU / ml, 250mIU / ml, 500mIU / ml, 1000mIU / ml, 2500mIU / ml, 5000mIU / ml, 7500mIU / ml, 10000mIU / ml, 15000mIU / ml, 20000mIU / ml, 25000mIU / ml ml.
[0103] The concentration of hepatitis B surface antibody to be tested is unknown.
[0104] Take equal amounts of hepatitis B surface antibody of different concentrations and the hepatitis B surface antibody to be tested and drop them on the test strips respectively.
[0105] The test strip is provided with a binding pad, a detection line and a control line.
[0106] The binding pad is coated with a first antigen labeled with a fluorescent marker and capable of binding to the hepatitis B surface antibody;
[0107] The detection line is coated with a second antigen immobilized on the detection line which is differ...
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