Mammal high altitude cerebral edema biomarker and application thereof

A high-altitude cerebral edema and biomarker technology, applied in the field of high-altitude cerebral edema biomarkers, can solve the problems of intestinal mucosal barrier function damage, increased mucosal permeability, and tissue cell damage in the body.

Inactive Publication Date: 2016-12-21
INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA
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Problems solved by technology

At the same time, the endotoxin produced by Gram-negative bacterial infection causes tissue cell damage and dysfunction in the body, damages the barrier function of the intestinal mucosa, increases the permeability of the mucosa, and leads to bacterial translocation

Method used

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  • Mammal high altitude cerebral edema biomarker and application thereof
  • Mammal high altitude cerebral edema biomarker and application thereof

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Embodiment Construction

[0012] The specific embodiments of the present invention will be described in detail below in conjunction with the accompanying drawings, but it should be understood that the protection scope of the present invention is not limited by the specific embodiments.

[0013] 100 male C57BL / 6 mice were taken, and 50 of them were placed in a hypobaric hypoxic chamber for plateau hypoxic treatment (simulated altitude of 6,000m). After 6 hours, plasma and brain tissue were collected to determine the level of endotoxin in mouse plasma and The determination of water content in brain tissue and the determination of plasma endotoxin in mice were carried out using the Limulus reagent produced by Xiamen Limulus Reagent Experimental Reagent Factory.

[0014] 1) The detection of plasma endotoxin content is carried out according to the instructions of the kit, and the steps are as follows:

[0015] 1. Configuration of Bacterial Endotoxin Standard Solution

[0016] The endotoxin solubility used ...

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Abstract

The invention discloses application of compound lipopolysaccharide of lipid and polysaccharide as a mammal high altitude cerebral edema biomarker. According to the invention, a limulus reagent is utilized for detecting content of lipopolysaccharide in blood plasma of a mammal after entering a high altitude low-oxygen environment, and when the content of the lipopolysaccharide in the blood plasma is more than 0.5EU / ml, content of water in brain tissues of the mammal is obviously increased. The content of the lipopolysaccharide in the blood plasma can be taken as the biomarker that high altitude cerebral edema of the mammal occurs, and an experimental basis is provided for early warning and diagnosing that the high altitude cerebral edema of the mammal occurs.

Description

technical field [0001] The invention belongs to the biomarker of high-altitude cerebral edema, and in particular relates to the application of lipopolysaccharide in detecting mammalian high-altitude cerebral edema. Background technique [0002] Acute mountain sickness mainly includes acute mountain sickness, high altitude pulmonary edema, and high altitude cerebral edema. Among them, acute mountain sickness has the highest incidence rate and the widest range of influence. Edema, increased intracranial pressure, etc. Among them, increased cerebral vascular permeability and imbalance of vasoconstriction and relaxation are important mechanisms of acute mountain sickness. Health of high altitude populations. Therefore, finding biomarkers of high-altitude cerebral edema is of great significance for the prediction and early warning of such diseases. [0003] Studies have found that: inflammatory mediators are important regulators of increased vascular permeability and unbalanced...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/579
CPCG01N33/579G01N2800/7095
Inventor 黄欣周延召赵名赵彤吴丽颖范明朱玲玲
Owner INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA
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