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Application of biochanin A in preparing drug for inhibiting proliferation and transfer of breast cancer, and breast cancer chemotherapy sensitizer

A technology of chickpein and breast cancer, which is applied in the field of biomedicine, can solve the problems of breast cancer chemotherapy resistance, lack of ZEB1, recurrence, etc., and achieve the effect of solving the problem of chemotherapy resistance

Inactive Publication Date: 2017-01-04
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the treatment of breast cancer has been greatly developed, many patients still die of chemotherapy resistance, recurrence and metastasis of breast cancer
[0003] Many studies have shown that ZEB1 plays an important role in the proliferation, metastasis and chemotherapy resistance of breast cancer, but there is still no ZEB1 inhibitor

Method used

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  • Application of biochanin A in preparing drug for inhibiting proliferation and transfer of breast cancer, and breast cancer chemotherapy sensitizer
  • Application of biochanin A in preparing drug for inhibiting proliferation and transfer of breast cancer, and breast cancer chemotherapy sensitizer
  • Application of biochanin A in preparing drug for inhibiting proliferation and transfer of breast cancer, and breast cancer chemotherapy sensitizer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Biochanin A inhibits the expression of ZEB1

[0031] After the MDA-MB-231 cells were treated with Biochanin A (Biochanin A) for 48 hours, the protein was extracted, and the expression level of ZEB1 was detected by Western Blot; at the same time, the immunohistochemical experiment of the in vivo experiment also explored the biochanin A (Biochanin A) Effect on the expression level of ZEB1.

[0032] The result is as figure 2 and Figure 7 as shown, figure 2 Western Blot results showed that the expression of ZEB1 was significantly inhibited after biochanin A (Biochanin A) treated MDA-MB-231 cells, Figure 7 The results of immunohistochemistry showed that the expression of ZEB1 in the tumors of the mice treated with biochanin A was significantly inhibited, which provided a new application for the preparation of ZEB1 inhibitors.

Embodiment 2

[0034] Biochanin A inhibits the proliferation of breast cancer cells

[0035] (1) 96-well plate, 3000 MDA-MB-231 cells per well, put the culture plate into the incubator.

[0036] (2) MDA-MB-231 cells were treated with Biochanin A (Biochanin A) or DMSO for 24h, 48h, and 72h, respectively.

[0037] (3) CCK-8 method was used to detect the inhibitory effect of biochanin A on breast cancer cells.

[0038] like image 3 As shown, biochanin A (Biochanin A) can significantly inhibit the proliferation ability of breast cancer cells.

Embodiment 3

[0040] Biochanin A inhibits the metastasis of breast cancer cells

[0041] (1) Take 200uL of MDA-MB-231 cell suspension and put it into the Transwell chamber, and treat it with biochanin A (Biochanin A) or DMSO respectively.

[0042] (2) Add 500uL full medium to the lower chamber of the 24-well plate, and culture for 48 hours.

[0043] (3) Wipe off the cells in the upper chamber with a cotton swab, stain with crystal violet for three hours, take pictures and count.

[0044] like Figure 4 As shown, biochanin A (Biochanin A) can significantly inhibit the metastatic ability of breast cancer cells.

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Abstract

The invention provides application of biochanin A in preparing drug for inhibiting proliferation and transfer of breast cancer, and preparing breast cancer chemotherapy sensitizer. The compound involved in the invention has the effect of reducing ZEB1 gene expression in breast cancer, and further inhibiting the proliferation, transfer and chemotherapy resistance of the breast cancer; biochanin A shows strong breast cancer inhibiting effect and chemotherapy sensitizing effect, and thereby proving a new strategy for the treatment of the breast cancer.

Description

technical field [0001] The present invention provides a new application of biochanin A (Biochanin A), which involves the production of biochanin A (Biochanin A) to inhibit ZEB1 (zinc finger-containing homeobox transcription regulator delta-globulin enhancer 1) The application of the drug for inhibiting the proliferation and metastasis of breast cancer and the preparation of chemotherapy sensitizers for breast cancer belongs to the field of biomedicine. Background technique [0002] Breast cancer has occupied the first place in the incidence of female malignant tumors worldwide, and has become one of the main causes of female malignant tumor death. Although the treatment of breast cancer has been greatly developed, many patients still die of breast cancer chemotherapy resistance, recurrence and metastasis caused by recurrence. [0003] Many studies have shown that ZEB1 plays an important role in the proliferation, metastasis and chemotherapy resistance of breast cancer, but ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/352A61P35/04
CPCA61K31/352
Inventor 杨爽张震
Owner NANKAI UNIV
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