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Use of pingbeimine B

A technology of Pingbeijing B and enterovirus, which is applied in the field of medicine and can solve problems such as teratogenicity, inhibition of growth and development, and strong side effects of drugs

Active Publication Date: 2017-01-04
JIANGSU KANION PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these broad-spectrum antiviral drugs have relatively strong side effects. Taking the most common ribavirin as an example, it has the risk of teratogenicity and growth inhibition when applied to infants.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Example 1 The protective effect of Pingbeijing B on EV71 and CoxA16 virus-infected cells

[0052] 1. Experimental materials:

[0053] Coxsackievirus A16 (CoxA16) and Enterovirus 71 (EV71), provided by the Institute of Microbial Epidemiology and Immunology, Academy of Military Medical Sciences of the Chinese People's Liberation Army, preserved by Jiangsu Kangyuan Modern Chinese Medicine Research Institute, cultured in Vero cells Passaged internally and stored at -80°C.

[0054] African green monkey kidney cells (Vero cells) were purchased from the ATCC cell bank in the United States and preserved by Jiangsu Kangyuan Modern Chinese Medicine Research Institute.

[0055] DMEM medium, purchased from Nanjing Kaiji Biotechnology Development Co., Ltd., batch number: 20141024, cell growth medium containing 10% fetal bovine serum, 1 × 10 5 U / L penicillin, 100mg / L streptomycin. The cell maintenance solution is the same as the cell growth solution except 2% fetal bovine serum. ...

Embodiment 2

[0062] Example 2 Therapeutic Effect of Oral Administration of Pingbeijing B on EV71 Infection

[0063] 1. Experimental materials:

[0064] Enterovirus 71 (EV71), provided by the Institute of Microbial Epidemiology and Immunology, Academy of Military Medical Sciences of the Chinese People's Liberation Army, preserved by Jiangsu Kangyuan Modern Chinese Medicine Research Institute, passaged in Vero cell culture, and preserved at -80°C.

[0065] SPF grade BALB / c pregnant mice with 15-16 days of pregnancy were purchased from Qinglongshan Experimental Animal Breeding Farm, Jiangning District, Nanjing, and were raised in an IVC system (temperature 18-28°C, relative humidity 40-70%, mechanical ventilation; Light and dark cycle: 12h / 12h; 150-300Lux illumination), 3-day-old newborn suckling mice were used for the experiment.

[0066] Pingbeijing B, with a purity of 98%, is commercially available. Ribavirin was purchased from Harbin Pharmaceutical Sanjing Pharmaceutical Co., Ltd., appr...

Embodiment 3

[0080] Example 3 Therapeutic Effect of Oral Administration of Pingbeijing B on CoxA16 Infection

[0081] 1. Experimental materials:

[0082] Coxsackievirus A16 (Cox A16) was provided by the Institute of Microbial Epidemiology and Immunology, Academy of Military Medical Sciences of the Chinese People's Liberation Army, preserved by Jiangsu Kangyuan Modern Chinese Medicine Research Institute, passaged in Vero cell culture, and preserved at -80°C.

[0083] SPF grade BALB / c pregnant mice with 15-16 days of pregnancy were purchased from Qinglongshan Experimental Animal Breeding Farm, Jiangning District, Nanjing, and were raised in an IVC system (temperature 18-28°C, relative humidity 40-70%, mechanical ventilation; Light and dark cycle: 12h / 12h; 150-300Lux illumination), 3-day-old newborn suckling mice were used for the experiment.

[0084] Pingbeijing B, with a purity of 98%, is commercially available. Ribavirin was purchased from Harbin Pharmaceutical Sanjing Pharmaceutical Co....

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Abstract

The invention relates to the technical field of medicines, in particular to use of pingbeimine B. The invention provides application of the pingbeimine B in preparation of medicines for preventing and / or treating hand-foot-and-mouth disease. The experimental results prove that the pingbeimine B has an inhibiting effect for cytopathy induced by enterovirus and can improve the survival rate of cells infected by the virus; furthermore, the pingbeimine B can inhibit virus reproduction in a body, increase the survival rate of animals infected by the virus, prolong the survival time of the animals infected by the virus and relieve the symptoms of disease caused by virus infection, thus having a function of treating the hand-foot-and-mouth disease; the pingbeimine B also can prevent the proliferation and spreading of the virus in the body and protect normal tissues from being hurt, thus having an effect of preventing virus infectious diseases.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the application of pingbeijing B. Background technique [0002] Hand, foot and mouth disease is a childhood infectious disease caused by a virus, and it is a legal Class C infectious disease in my country. The disease mainly infects infants and young children aged 0 to 6, and children aged 2 to 3 are the most common infection. In the early stage of hand, foot and mouth disease, herpes or ulcers are caused on the extremities and mouth of children, and most children develop within 1 to 2 weeks. get well. However, a small number of cases progress rapidly, and meningitis, aseptic encephalitis, brainstem encephalitis, encephalomyelitis, pulmonary edema, and circulatory disturbance may occur within 1 to 5 days of onset. Death and sequelae may be left in surviving cases. [0003] According to the report of the World Health Organization, there are about 20 kinds of viruses that can c...

Claims

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Application Information

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IPC IPC(8): A61K31/4375A61P31/14
CPCA61K31/4375
Inventor 萧伟丁玥曹泽彧曹亮王振中丁岗胡晗绯
Owner JIANGSU KANION PHARMA CO LTD
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