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Culture medium for screening blakeslea trispora capable of producing high-yield lycopene

A technology based on lycopene and burlella mold, applied in the direction of microorganism-based methods, fungi, and isolated microorganisms, can solve the problems of difficult colony separation, low lycopene, and vigorous production, and prevent mycelium from spreading , High growth and reproduction, fast growth effect

Inactive Publication Date: 2017-01-04
丽江映华生物药业有限公司
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Problems solved by technology

However, when the existing medium culture screens high-yield strains of B. trispora, it is in a sprawling growth state, and the production is too vigorous. It is difficult to separate the colony formed by a single spore, which brings difficulties to the selection of a single colony strain and is difficult to obtain. High lycopene-producing strains of B. trispora lead to low lycopene in fermentative production

Method used

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  • Culture medium for screening blakeslea trispora capable of producing high-yield lycopene
  • Culture medium for screening blakeslea trispora capable of producing high-yield lycopene

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Embodiment Construction

[0025] Below in conjunction with embodiment the present invention is further described.

[0026] 1. Preparation of medium: take the preparation of 500ml medium as an example: roughly take potatoes according to the proportion, peel and cut into pieces (about 3cm×3cm×1cm), then accurately weigh 200g, boil for 30 minutes, filter, add accurate Measure 10g of glucose, 0.25g of sodium deoxycholate, and 4g of agar, mix well, add water to make up volume, bottle, seal, and sterilize at 121°C for 20-30 minutes. Take it out and cool it down to a temperature that can be tolerated by bare hands, pour it into a sterilized plate, let it stand, and cool it down.

[0027] 2. Incline culture: Pick a ring of mycelium in the slope and put it into a triangular flask filled with sterilized saline and small glass beads, shake and mix, and dilute to 10 -3 -10 -7 , spread on a flat plate, and cultivate at 27-28°C with a relative humidity of about 50%. When colonies appear, select a single colony an...

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Abstract

The invention provides a culture medium for screening blakeslea trispora capable of producing high-yield lycopene. The culture medium is prepared from glucose, potatoes, agar and water. The culture medium is characterized in that deoxysodium cholate is added, and the culture medium is prepared from, by mass, 2.0%-2.5% of glucose, 20%-25% of potatoes, 0.05%-0.06% of deoxysodium cholate, 2.0%-2.5% of agar and the balance water. After deoxysodium cholate is added, the culture medium effectively prevents mould hypha spreading, inhibits growth of Gram-positive bacteria, helps to obtain an independent single colony in the process of culturing screened seeds and screens out blakeslea trispora capable of producing high-yield lycopene.

Description

technical field [0001] The invention belongs to the technical field of fungal culture, and in particular relates to a screening medium for B. trispora. Background technique [0002] Lycopene is a natural pigment contained in plants, mainly in the ripe fruit of tomato, a plant of the family Solanaceae. It is an intermediate in the biosynthesis of many carotenoids. Its antioxidant properties are the strongest among all carotenoids, and its ability to scavenge singlet oxygen is 100 times that of vitamin E, a commonly used antioxidant, and more than 2 times that of β-carotene; lycopene has superior physiological functions , it not only has the effect of anti-cancer and tumor suppression, but also has great significance for preventing various adult diseases such as cardiovascular disease and arteriosclerosis, enhancing the human immune system and delaying aging. It is a promising new functional Natural colouring. [0003] Blakeslea trispora Blakeslea trispora It is Choanephor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/02C12R1/645
CPCC12N1/02C12N1/14
Inventor 王钱钢杨宗禄张小元张燕唐金祥和银权
Owner 丽江映华生物药业有限公司
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