A kind of assay method of sesquiterpenoids in tobacco

A technology of sesquiterpenoids and determination method, which is applied in the field of analytical chemistry to achieve the effects of good effect, good precision and wide linear range

Active Publication Date: 2018-06-12
CHINA TOBACCO YUNNAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

We have established a method for the determination of sesquiterpenoids, an important sesquiterpenoid component in tobacco, by gel purification of samples and GC-MS analysis. At present, this method has not been reported in relevant literature

Method used

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  • A kind of assay method of sesquiterpenoids in tobacco
  • A kind of assay method of sesquiterpenoids in tobacco
  • A kind of assay method of sesquiterpenoids in tobacco

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Select flue-cured tobacco leaves, accurately weigh 1.0g of the sample, add 20mL of ethyl acetate and reflux for extraction for 20min. The extract is concentrated to 0.5mL and added to the packed gel column (the material is dextran gel, the gel column The packing size is 0.6×150mm, the packing solvent is ethyl acetate), the loaded gel column is eluted with ethyl acetate, and the eluted fractions of 10-11 times the column volume are collected and concentrated to 0.5mL again; press selected Chromatographic conditions and mass spectrometry conditions determined that the content of sesquiterpenoids sesquiterpenoids was 6.26μg / g.

Embodiment 2

[0034] Select oriental tobacco leaves, accurately weigh 0.50g of the sample, add 20mL of acetone for reflux extraction for 20min, concentrate the extract to 0.5mL, and add it to the packed gel column (the material is dextran gel, the filling of the gel column The size is 0.6×150mm, and the packing solvent is acetone), the gel column that has been loaded is eluted with acetone, and the eluted fractions of 10 to 11 column volumes are collected and concentrated to 0.5 mL; according to the selected chromatographic conditions and mass spectrometry Condition determination, the content of sesquiterpenoids sesquiterpenoids is 11.2μg / g.

Embodiment 3

[0036] The sample is selected as the white tobacco leaf. Weigh accurately 1.0g of the sample, add 20mL of ethanol for reflux extraction for 20min, concentrate the extract to 0.5mL, and add it to the packed gel column (the material is dextran gel, gel The packing specification of the column is 0.6×150mm, and the packing solvent is ethanol. The gel column that has been loaded is eluted with ethanol, and the eluted fractions of 10 to 11 column volumes are collected and concentrated to 0.5 mL again; according to the selected chromatogram According to the conditions and mass spectrometry conditions, the content of sesquiterpenoids is 4.26μg / g.

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Abstract

The invention discloses a method for measuring sesquiterpenoids in tobacco. The method for measuring sesquiterpenoids in tobacco comprises the following steps: using an organic solvent to extract a sample; purifying the sample through gel column chromatography; adopting gas chromatography mass spectrometry (GC-MS) to analyze the sample purified through gel column chromatography; and measuring the content of the sesquiterpenoids through selected ion monitoring. The invention further discloses application of the measuring method to evaluation of the tobacco style and the tobacco quality.

Description

Technical field [0001] The invention belongs to the technical field of analytical chemistry, and specifically relates to a method for determining sesquiterpenoids in tobacco. Background technique [0002] Smoking is an indispensable part of the cultural life of hundreds of millions of smokers, and it is also an important source of national and local finances and taxes. Although smoking has been widely recognized as harmful to health, tobacco still has a strong appeal to thousands of consumers. In addition to the addictive nature of nicotine, the rich flavors in tobacco also play an important role. [0003] The aroma components in tobacco have always been research hotspots at home and abroad. The research on tobacco aroma components abroad is mainly concentrated in the 1970s. The Swedish scholar Hlubucek JR and others used phytochemical methods to separate a series of new compounds from tobacco. These research results Published a series of research papers in "Acta Chemica Scandinav...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 杨光宇申钦鹏刘春波唐石云刘志华姚守拙姜瑞石倩倩张凤梅司晓喜何沛缪明明
Owner CHINA TOBACCO YUNNAN IND
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