55results about How to "To achieve the purpose of separation and purification" patented technology

The invention discloses a method for extracting coenzyme Q10 from microorganisms, wherein the ultrasonic disintegration is to mix microorganisms with the ability of producing the coenzyme Q10 and an organic solvent in which the coenzyme Q10 is well dissolved according to the mass volume ratio of 1-5 to 10-20, and to obtain the coenzyme Q10 after ultrasonication at a temperature of 0 DEG C, wherein the ultrasonic frequency is between 0.2 and 0.8 and the power is between 300 and 500 watts; and the alkaline splitting method is to extract the coenzyme Q10 from fermentation broth of Rhodobacter sphaeroides. The microorganisms are preferred to be microorganisms of the Rhodobacter sphaeroides. The method for extracting the coenzyme Q10 has the advantages of high efficiency, low pollution, low requirements on apparatuses and equipment, low cost and so on, and is suitable for promotion and application.

A process technology extracting the tea polyphenols from the tea plant flowers is provided, which relates to the preparation technology of the separation and purification of the tea polyphenols. The process technology comprises the following steps: 1) drying the tea tree pollen; 2) producing the extracting solution by ultrasonic wave; 3) producing ultrafiltration treatment filter liquor after extraction, condensation, microfiltration and ultrafiltration; 4) gaining polyphenols extracting solution through absorption by medium polarity adsorbent resins; 5) producing the extracting solution to the extract and producing the extract to the polyphenol fine powder of the tea plant flowers. Compared with the method using the current tea to prepare tea polyphenols, the invention has the following advantages that aiming at the characteristics of abundant content of the polyphenols of the tea plant flowers, little pigment impurity and good stability, the invention carries out the comprehensive development and use to change waste into valuable, replaces the tea leaves to extract the natural tea polyphenols and provides raw materials and additives with high quality for the industries such as medicine, food and cosmetic. The invention improves the utilization rate of the tea plant flowers resources and is suitable for the scale production, reduces the energy consumption and production cost and enhances the competitiveness of the production.

The invention relates to a method for extracting rose flavone from a rose residue solution. The method comprises the following steps: (1), filtering the rose residue solution, concentrating, adding ethyl alcohol to adjust the pH value of the solution, refrigerating and standing in the absence of light; (2), performing suction filtration on the solution in the step (1), and concentrating, so as to obtain rose flavone pigment coarse powder; (3), performing separation and purification on the rose flavone pigment coarse powder obtained in the step (2), so as to obtain eluant; (4), concentrating and drying the eluant, so as to obtain dry flavone. According to the invention, the flavone extraction ratio reaches above 8% to the maximum extent, and is greatly improved compared with the flavone extraction ratio being not higher than 5% in the prior art; the obtained flavone is bright red in color, and can be widely applied to the fields of medicine and food.

The invention discloses a method for preparing carbon quantum dots by virtue of polyethyleneimine decomposition, belonging to the technical field of preparation of nanometer materials. The method comprises the steps of dispersing polyethyleneimine as a raw material into an ethanol solvent, adequately stirring, carrying out heat treatment at 180-200 DEG C for 20 hours in a reaction kettle, and naturally cooling, so as to obtain decomposed auto-fluorescent polyethyleneimine. The method disclosed by the invention has the advantages that the synthetic process is simple and convenient and high in efficiency; polyethyleneimine simultaneously servers as a carbon source and a nitrogen source, so that obtained carbon quantum dots have high impurity and moderate particle sizes, are monodisperse and water soluble and are expected to be applied to the LED membrane materials, fluorescent marks, DNA carriers and targeted drug delivery particles.

The invention discloses a method for producing food-grade potassium chloride by adopting an adsorption process. The method comprises steps such as dissolving, filtering, multilevel macroporous resin adsorption purification, active carbon adsorption, concentration crystallization, solid-liquid separation, drying and the like. Octadecylamine, dodecylmorpholine and Number 2 oil in the industrial potassium chloride are removed by virtue of adsorption purification of macroporous resin and active carbon, so that the purposes of purifying the industrial potassium chloride and producing the food-grade potassium chloride can be realized.

The invention provides a herbage plant polysaccharide purification method by utilization of a microwave-assisted extraction and integration flocculation technology. The method includes that: step one, crushing herbage plant into powder, and stirring and mixing the power by adding water; step two, placing mixture obtained in the first step into a microwave extraction device, irradiating the mixture for 2-3 minutes through microwave, cooling the mixture, and obtaining extraction solution containing polysaccharide in a pressure-reducing and filtration-extracting mode; step three, concentrating the extraction solution obtained in the second step, adding ZTC flocculant under a certain rotation speed to perform flocculation, filtering and removing impurity floc after the flocculation is finished, and pure polysaccharide solution is obtained. According to the herbage plant polysaccharide purification method by the utilization of the microwave-assisted extraction and integration flocculation technology, a microwave heating technology is adopted, heating is simultaneously performed for interior and exterior of extracted matters, the herbage plant polysaccharide purification method by the utilization of the microwave-assisted extraction and integration flocculation technology is high-efficient, fast, saved in solvent consumption and reduced in energy consumption; and flocculant electricity neutralization and bridge adsorption effects are utilized to remove foreign particles in the extraction solution, cost is low, foreign particle removing rate is high, and obtained products are high in quality.

A process for renaturating and purifying the inclusion body protein includes such steps as dissolving the inclusion body protein in acidic / alkaline flowing phase or other solution, loading it in the ion-exchange chromatographic column, reversible adsorbing it by the chromatographic medium, and modifier concentration gradient elution. Its advantages are high concentration and activity of recovered protein and high purity.

The invention provides a range hood provided with a multi-layer purifying and adsorbing device. The range hood comprises a dynamic centrifugal adsorbing device, an air channel condensing and adsorbing device and an electrostatic adsorbing device which are sequentially arranged from a cooking fume suction direction to a cooking fume exhaust direction; the dynamic centrifugal adsorbing device comprises a rotatable centrifugal mesh disc; the electrostatic adsorbing device can form a high-voltage electric field for adsorbing cooking fume particles; and the air channel condensing and adsorbing device is used for conveying cooking fume and condensing and adsorbing the cooking fume in a conveying process. According to the range hood provided by the invention, at least three layers of adsorbing devices are arranged for adsorbing and purifying the cooking fume particles, therefore the indexes such as air volume of the range hood reach the standards, the PM2.5 emission index is also reduced more effectively, and the overall environment of a kitchen and air is improved.

The invention discloses a purification method of polysilicon by-product silicon tetrachloride. The purification method comprises the following step: adsorbing the polysilicon by-product silicon tetrachloride by amphoteric ion exchange resin to obtain purified silicon tetrachloride. In the purification method, the amphoteric ion exchange resin is adopted, so that the impurity content can be reduced, and the purity of polysilicon by-product silicon tetrachloride is improved effectively. Meanwhile, the amphoteric ion exchange resin can be used stably for a long time, so that the amount of resin used during long-term operation can be reduced, and the treatment cost of waste resin after use can be lowered. The purification method is simple, is low in energy consumption, is low in cost, and is conducive to the reuse of silicon tetrachloride.

The invention provides a kit for extracting stable bacterial genome DNA by a paramagnetic particle method and an extraction method thereof. The kit comprises a lysis reagent group for lysing and releasing bacterial genome DNA and an extraction reagent group for extracting the genome DNA, wherein the lysis reagent group consists of a buffer solution and a lysis solution; the extraction reagent group consists of a paramagnetic particle binding solution, a washing solution and an eluent; and the paramagnetic particle binding solution consists of sodium salt sodium citrate or sodium acetate, a reducing agent dithiothreitol, guanidine hydrochloride and a paramagnetic particle suspension. The raw materials are reasonably compounded to obtain the optimal proportion and the combined extraction conditions of a paramagnetic particle binding solution compounding system, and the extraction method is optimized, so that the extracted bacterial genome DNA has high purity and complete fragments, is difficult to degrade, has stability far superior to that of the commercially available kit, and has remarkable advantages in subsequent experiments such as DNA storage, hybridization, PCR and the like; and meanwhile, the reagents are nontoxic and have small environmental pollution.

The invention discloses a preparation method of a starch zeolite molecular sieve flocculating agent. The preparation method comprises the steps of (1) adding distilled water into 2.0-10.0g of cassava starch, heating until boiling, and centrifuging at the rotating speed of 4500r / min for 10min after cooling to obtain a cassava starch solution; (2) stirring 30.0-50.0g of epidesmine powder and 90-150ml of HCl solution with the mass percent concentration of 15% at the temperature of 70-90 DEG C for 1-3h, filtering, washing until no Cl<-> exists, and drying to form acidized zeolite; (3) mixing 6.0-10.0g of NaOH and 3.0-5.0g of NaAlO2, adding 144-240ml of water, adding 3.0-5.0g of acidized zeolite, stirring, crystallizing, filtering, washing and drying to obtain a zeolite A molecular sieve; (4) keeping 20mL of cassava starch solution and 2.5-3.5g of zeolite A molecular sieve at the temperature of 10-30 DEG C for 1-2h, centrifuging at the rotating speed of 4500r / min for 10min, and settling. The starch zeolite molecular sieve flocculating agent prepared by using the preparation method is favorable in performance, is nontoxic and harmless and has a broad spectrum effect; when the flocculating agent is used for purifying water, the water purification rate is up to 97.41%.

The invention relates to a method for purifying phosphogypsum by using hydrocyclone. The method comprises the following steps: mixing the phosphogypsum with water according to the weight part ratio of 5-40: 60-95, and then grinding the mixture into slurry; sieving the slurry obtained in the step A by using a square hole sieve with meshes of 40-100, and stirring the slurry capable of passing through the sieve in a stirring pond; pumping the slurry in the stirring pond in the hydrocyclone while stirring, and purifying and separating the slurry by the hydrocyclone under the condition that the pressure of a feeding opening of the hydrocyclone is 0.1-0.4 Mpa so that impurities which are separated out flow out from an overflowing opening of the hydrocyclone along with the water and enter a settling pond and the high-purity phosphogypsum obtained by purification flows out from a discharging opening of the hydrocyclone; and naturally filtering out water from the phosphogypsum slurry obtained by purification or centrifugally separating the phosphogypsum slurry to obtain the high-purity phosphogypsum. By the method, water consumption in a purification process can be reduced.

The invention discloses equipment and a method for preparing high-purity tellurium. The equipment comprises a vacuum distillation device, a vacuumizing device, a hydrogen purification circulating device, a high-frequency induction heating device and a product collecting device; the vacuumizing device is used for providing a vacuum environment and comprises a single-hole vacuum suction filter and adouble-hole vacuum suction filter, and the single-hole vacuum suction filter is connected with an extraction opening in a vacuum distillation kettle; and the hydrogen purification circulating deviceis used for providing a hydrogen reduction environment for the product collecting device. When the equipment is used for preparing high-purity tellurium, the process is short, the energy consumption is low, the tellurium remelting procedure in the conventional high-purity tellurium preparation process is omitted, and the problems of high energy consumption caused in the remelting process and localoxidation caused in the charging process are avoided.

The invention discloses hydrophobic protein separation and purification method. The method comprise: soaking solid substrate with hydrophobic particle on surface in hydrophobic protein solution, then taking the substrate out of the hydrophobic protein solution, rinsing, carrying out supersonic oscillation in container filled with water, then taking the substrate out of the solution, obtaining purified hydrophobic protein solution. The principle that hydrophobic protein can adsorb at hydrophobic substance surface, and hydrophobic particle has hetero phase nucleation growth effect to hydrophobic protein can be utilized to achieve the aim for separating and purifying the hydrophobic protein. The inventive method is simply and conveniently operated, and cost is low. The hydrophobic substance surface can be repeatedly used which is a merit for industrial production. The present invention will have a broad prospect in hydrophobic protein separation and purification field.

The invention discloses a new lithium sulfate leaching agent separation and purification technology. The technology is characterized by comprising following steps: firstly, a microfiltration system is used for pretreating a lithium sulfate leaching agent, impurities and suspended matter in feed liquid are removed, and microfiltration dialyzate is obtained; secondly, the microfiltration dialyzate enters a nanofiltration system to obtain nanofiltration dialyzate and a nanofiltration concentrated solution; thirdly, the nanofiltration concentrated solution enters an electrodialysis system to be concentrated, electrodialysis concentrated water and electrodialysis contributing water are obtained, the electrodialysis concentrated water is used for MVR evaporation, and solid sulfuric acid lithium is obtained; and fourthly, the electrodialysis contributing water uses reverse osmosis for deep treatment, reverse osmosis concentrated water returns to the third step to be continuously concentrated, and reverse osmosis contributing water returns to the production technology. According to the lithium sulfate leaching agent separation and purification technology, through microfiltration, nanofiltration and electrodialysis concentration, the separation and purification aim of lithium sulfate is achieved; meanwhile, due to adding of chemical reagents, the evaporation cost is reduced, the recycling water amount is improved, and the outward zero emission of the whole system is achieved.

The invention provides an application of a silica-gel chromatographic column to detection of high-purity gas via gas chromatography, and relates to a method and a device for detecting high-purity gas by using the silica-gel chromatographic column. The application of the silica-gel chromatographic column to detection of the high-purity gas has the characteristics that the perfect chromatographic peak shape is provided, high sensitivity and good selectivity are obtained, the sharp peak is realized, the peak capacity is high and the like.

The invention discloses a ganoderma lucidum spore powder wall breaking technology. The technology comprises the following steps that S1, a ganoderma lucidum spore dry product is taken and crushed into ganoderma lucidum spore powder in an ultrafine crusher, the powder is placed into a closed container, saturated water vapor is introduced at the normal temperature for humidifying, the power is left to stand for 5-6 h at the temperature ranging from subzero 15 DEG C to subzero 20 DEG C then is crushed into powder of 1-1.5 [mu]m; S2, the material obtained in S1 is placed into a jet mill, then ceramic powder is added, and jet milling is performed under the condition of 2-5 DEG C to obtain a wall-broken ganoderma lucidum spore powder mixture; S3, the wall-broken ganoderma lucidum spore powder mixture obtained in S2 is introduced into a separation device for separation, and wall-broken ganoderma lucidum spore powder and a spore wall mixture are collected; and S4, the collected wall-broken ganoderma lucidum spore powder is dried to obtain a finished product. According to the ganoderma lucidum spore powder wall breaking technology, the wall breaking rate and the effective component content of the ganoderma lucidum spore powder can be effectively increased, the anti-oxidation effect is guaranteed, meanwhile, water is prevented, and thus the shelf life is effectively prolonged.

The invention relates to a gas cleaning device, the structure of a cleaner is modified from internal circulation of a normal cleaner to be extracorporeal circulation and from a spray head of a normal cleaner to be a dousing tank, which is equipped with a water-curtain wall baffle plate, gas is forced to reversely pass through the water-curtain wall, and water consumption of spraying and pool volume of spraying are enlarged, spraying water which is used gets into the spraying pool, coke tar and impurity substance are condensed, coke tar floats on water surface, which is put into a coke tar pool to store and sale after gathering. Impurity substance is settled at the bottom of the pool, which is cleaned and stored when changing water, and is returned to field with plant ash.

The invention provides a method for separating and purifying ursolic acid from rosemary, wherein the method comprises the following steps: 1) taking dried rosemary leaves, carrying out reflux extraction on the dried rosemary leaves by an ethanol-water solution, and filtering to obtain a filtrate 1; concentrating the filtrate 1 under reduced pressure, adding water, and standing for cooling; filtering to obtain a filter cake 1, and stirring the filter cake 1 with an acid-ethanol-water solution; 2) filtering to obtain a filter cake 2, and carrying out reflux extraction on the filter cake 2 by analkali-ethanol-water solution; and filtering to obtain a filtrate 2, and adding activated carbon into the filtrate 2, and decolorizing; and 3) filtering to obtain a filtrate 3, adding an acid-water solution into the filtrate 3 until no precipitate appears, carrying out suction filtration to obtain a filter cake 3, and repeatedly crystallizing the filter cake 3 by an ethanol-water solution. According to the method, a pickling step is added in the separation and purification process by utilizing different dissolvability, impurities with polarity larger than that of ursolic acid and similar to that of ursolic acid can be effectively removed in the pickling step, a great purification effect is achieved on the subsequent acid precipitation step, the recrystallization frequency can be reduced, and high-purity ursolic acid is obtained.

The invention relates to a separation and purification method of acetylacetone salt and a solvent used by the separation and purification method. The solvent comprises 30-70 parts of main solvent, 10-20 parts of auxiliary solvent, 0.1-1 part of solubilizer, 0.1-1 part of dispersing agent and 0.1-1 part of stabilizer. The separation and purification method includes the steps of firstly, sequentially adding the main solvent and the auxiliary solvent into a reaction container, stirring, sequentially adding the dispersing agent, a cosolvent and the stabilizer while stirring after the main solvent and the auxiliary solvent are evenly mixed, and evenly mixing to obtain a mixed solvent system; secondly, adding crude acetylacetone salt into the mixed solvent system obtained in the first step to dissolve the crude acetylacetone salt, and filtering the solution to obtain filtrate; thirdly, distilling of the filtrate to obtain solid, namely pure acetylacetone salt. The purity of the acetylacetone salt separated and purified by the method can reach above 99%.

The invention discloses a method for extracting coenzyme Q10 from microorganisms, wherein the ultrasonic disintegration is to mix microorganisms with the ability of producing the coenzyme Q10 and an organic solvent in which the coenzyme Q10 is well dissolved according to the mass volume ratio of 1-5 to 10-20, and to obtain the coenzyme Q10 after ultrasonication at a temperature of 0 DEG C, wherein the ultrasonic frequency is between 0.2 and 0.8 and the power is between 300 and 500 watts; and the alkaline splitting method is to extract the coenzyme Q10 from fermentation broth of Rhodobacter sphaeroides. The microorganisms are preferred to be microorganisms of the Rhodobacter sphaeroides. The method for extracting the coenzyme Q10 has the advantages of high efficiency, low pollution, low requirements on apparatuses and equipment, low cost and so on, and is suitable for promotion and application.

The invention relates to the technical field of environmental protection and resource recovery, in particular to a method and device for recovering and purifying phosphorus from sludge incineration ash. The method comprises the following steps: acid extraction: pickling the sludge incineration ash, and conducting filtering to obtain an acid extract; and electrochemical purification: with the acidextract as an anolyte, a nitrate-containing salt solution as a catholyte and inert electrodes as an anode and a cathode, arranging a cation exchange membrane between the anolyte and the catholyte, andcarrying out an electrochemical reaction, wherein the anolyte after the electrochemical reaction is a purified phosphate radical solution. The method can realize efficient recovery and high purification of the phosphorus element in the sludge incineration ash, is high in operability, and has good industrial application prospects.

The invention relates to a dairy farm sewage treatment device and a treatment method thereof. The dairy farm sewage treatment device comprises a solid-liquid separation device, an osmotic membrane filtration device, an ultrasonic sterilization actuator and a waste storage tank; waste in the waste storage tank is conveyed to the solid-liquid separation device for solid-liquid separation, and wastewater mixture separated from the solid-liquid separation is conveyed to the osmotic membrane filtration device for filtration, and clear liquid from the osmotic membrane filtration device can be conveyed to the ultrasonic sterilizer actuator for sterilization; and the dairy farm sewage treatment device realizes the efficient separation and degradation of pollutants, and residual colon bacillus inthe clear liquid is treated through an ultra-high power ultrasonic, the sterilization rate reaches 100%, the whole treatment system is convenient to maintain, the treatment cost is lowered, and the operation and management are simple and convenient.

The invention belongs to the field of non-ferrous metal hydrometallurgy, and particularly relates to a method for efficiently recovering copper and tungsten from copper-tungsten alloy waste. The method comprises the following steps that the copper-tungsten alloy waste is crushed, mixed evenly with sodium hydroxide, sodium carbonate and borax, a small amount of water is further added, and the temperature is raised to 300-500 DEG C, so that the sodium hydroxide is in a molten state, hydrogen peroxide is dropped slowly, after the reaction is complete, a material is solidified, a proper amount ofhot water is added to dissolve the material, solid and liquid are separated, tungsten enters a liquid phase in the form of sodium tungstate, and copper remains in a solid phase in the form of the copper and copper oxide. Qualified sodium tungstate products are obtained by concentrated crystallization of the sodium tungstate, and the copper oxide is reduced by hydrogen to obtain copper powder. Thetechnical process is simple, the treatment cost is low, the recovery rate is high, and the copper-tungsten recovery rate is greater than 99%.

The invention discloses a method for measuring sesquiterpenoids in tobacco. The method for measuring sesquiterpenoids in tobacco comprises the following steps: using an organic solvent to extract a sample; purifying the sample through gel column chromatography; adopting gas chromatography mass spectrometry (GC-MS) to analyze the sample purified through gel column chromatography; and measuring the content of the sesquiterpenoids through selected ion monitoring. The invention further discloses application of the measuring method to evaluation of the tobacco style and the tobacco quality.

The invention discloses a method for comprehensively extracting saponin and polyphenol from Camellia oleifera Abel. cakes. The method comprises the following steps: (1) extracting the saponin and thepolyphenol in the Camellia oleifera Abel. cakes by utilizing an ethanol-water system; (2) filtering an extracting solution through a ceramic membrane; (3) after concentrating ceramic membrane permeated liquid, regulating the pH (Potential of Hydrogen); then adsorbing by adopting macroporous resin and eluting by utilizing water, an NaOH solution and an ethanol solution respectively; (4) regulatingthe pH of an NaOH eluting solution and concentrating by a nano-filtration membrane; drying to obtain the polyphenol; (5) concentrating an ethanol eluting solution until no alcohol flavor is formed, and drying to obtain the saponin. According to the method disclosed by the invention, a membrane separation and macroporous resin adsorption technology is adopted, and the influences of macromolecular compounds including polysaccharides, protein, grease, pigments and the like, and impurities including salt and the like in a traditional method on saponin and polyphenol separation and purification areovercome; the method has the characteristics of simple equipment operation, moderate conditions, easiness for controlling, simple process route, high product purity and the like.

The invention provides a method for recovering artemisinin from artemisinin recrystallization waste, and concretely relates to the technical field of artemisia carvifolia production. The method crystallizes recrystallization waste twice by using a mixed solution of petroleum ether and ethyl acetate. After the first crystallization, the recrystallization waste is cleaned by using an appropriate mixed solution of petroleum ether and ethyl acetate to improve the purity of an artemisinin first crystal. During the second crystallization, chlorobutanol is added as a crystallization aid, and refrigeration crystallization is combined to increase the amount of artemisinin crystallization precipitation, fully recover the artemisinin in the waste and increase the purity of crude products. Meanwhile,after the crude products obtained by the twice crystallization are dissolved, a mixture of microcrystalline cellulose and activated carbon is added to improve the impurity removal effect and the purity of a fine product. The method of the invention is stable in process, controllable in parameters and simple in steps, can effectively save pharmaceutical costs and improve the utilization rate of theraw materials, can increase the yield of artemisinin by more than 0.5%, and is suitable for large-scale production and popularization.

The invention discloses a compound feed prepared from effective components in tea leaves, and belongs to the field of feeds. The key points of the technical scheme are as follows: S1, selecting non-rotten and non-withered tea leaves, drying, performing crushing, performing screening to obtain 40-50-mesh tea leaves, and performing hot water extraction on the tea leaves according to a solid-liquid ratio of the tea leaves to distilled water of 1:20 to 1:25 to obtain a filtrate; and S2, performing adsorption, desorption and vacuum distillation on the filtrate by using macroporous resin, shaking for 10-15 S by using a constant-temperature oscillator every 10 min during adsorption, and performing desorption drying on the obtained mixed solution to obtain the extract. The compound feed disclosed by the invention has the advantages that: effective components in the tea leaves are extracted by adopting the macroporous resin, so that the yield of the extracted effective components in the tea leaves is relatively high, and the active substances in the tea leaves cannot be damaged.

The invention relates to the technical field of environmental protection and resource recovery, in particular to a method and device for recovering and purifying phosphorus from sludge incineration ash. The method comprises the following steps: acid extraction, pickling the sludge incineration ash, and filtering to obtain an acid extract; electrochemical purification, using the acid extract as an anolyte, a salt solution containing nitrate as a catholyte, and an inert electrode It is an anode and a cathode, and a cation exchange membrane is arranged between the anolyte and the catholyte to carry out an electrochemical reaction, and the anolyte after the electrochemical reaction is a solution of purified phosphate radicals. The method can realize high-efficiency recovery and high-level purification of phosphorus in sludge incineration ash, has strong operability, and has good industrial application prospects.

The invention belongs to the technical field of recombinant protein separation and purification, and mainly relates to a method for separating and purifying beta-glucosidase fusion protein, in particular to a method for constructing a foam separation system by using hydrophobic label performance and separating and purifying beta-Glu fusion protein by combining the temperature-sensitive performance of fusion enzyme. The invention aims to overcome the defects of complex extraction process, low separation and purification efficiency, high cost and the like in the existing protein separation and purification method, and provides a process for separating and purifying beta-glucosidase fusion protein by combining foam separation and inverse transition cycling (ITC). The process has the advantages of simple equipment, low investment, low energy consumption, no pollution and the like, is good in separation effect and low in enzyme activity loss, and is of great practical significance.