55results about How to "To achieve the purpose of separation and purification" patented technology

The invention discloses a method for extracting coenzyme Q10 from microorganisms, wherein the ultrasonic disintegration is to mix microorganisms with the ability of producing the coenzyme Q10 and an organic solvent in which the coenzyme Q10 is well dissolved according to the mass volume ratio of 1-5 to 10-20, and to obtain the coenzyme Q10 after ultrasonication at a temperature of 0 DEG C, wherein the ultrasonic frequency is between 0.2 and 0.8 and the power is between 300 and 500 watts; and the alkaline splitting method is to extract the coenzyme Q10 from fermentation broth of Rhodobacter sphaeroides. The microorganisms are preferred to be microorganisms of the Rhodobacter sphaeroides. The method for extracting the coenzyme Q10 has the advantages of high efficiency, low pollution, low requirements on apparatuses and equipment, low cost and so on, and is suitable for promotion and application.

A process technology extracting the tea polyphenols from the tea plant flowers is provided, which relates to the preparation technology of the separation and purification of the tea polyphenols. The process technology comprises the following steps: 1) drying the tea tree pollen; 2) producing the extracting solution by ultrasonic wave; 3) producing ultrafiltration treatment filter liquor after extraction, condensation, microfiltration and ultrafiltration; 4) gaining polyphenols extracting solution through absorption by medium polarity adsorbent resins; 5) producing the extracting solution to the extract and producing the extract to the polyphenol fine powder of the tea plant flowers. Compared with the method using the current tea to prepare tea polyphenols, the invention has the following advantages that aiming at the characteristics of abundant content of the polyphenols of the tea plant flowers, little pigment impurity and good stability, the invention carries out the comprehensive development and use to change waste into valuable, replaces the tea leaves to extract the natural tea polyphenols and provides raw materials and additives with high quality for the industries such as medicine, food and cosmetic. The invention improves the utilization rate of the tea plant flowers resources and is suitable for the scale production, reduces the energy consumption and production cost and enhances the competitiveness of the production.

The invention relates to a method for extracting rose flavone from a rose residue solution. The method comprises the following steps: (1), filtering the rose residue solution, concentrating, adding ethyl alcohol to adjust the pH value of the solution, refrigerating and standing in the absence of light; (2), performing suction filtration on the solution in the step (1), and concentrating, so as to obtain rose flavone pigment coarse powder; (3), performing separation and purification on the rose flavone pigment coarse powder obtained in the step (2), so as to obtain eluant; (4), concentrating and drying the eluant, so as to obtain dry flavone. According to the invention, the flavone extraction ratio reaches above 8% to the maximum extent, and is greatly improved compared with the flavone extraction ratio being not higher than 5% in the prior art; the obtained flavone is bright red in color, and can be widely applied to the fields of medicine and food.

The invention discloses a method for preparing carbon quantum dots by virtue of polyethyleneimine decomposition, belonging to the technical field of preparation of nanometer materials. The method comprises the steps of dispersing polyethyleneimine as a raw material into an ethanol solvent, adequately stirring, carrying out heat treatment at 180-200 DEG C for 20 hours in a reaction kettle, and naturally cooling, so as to obtain decomposed auto-fluorescent polyethyleneimine. The method disclosed by the invention has the advantages that the synthetic process is simple and convenient and high in efficiency; polyethyleneimine simultaneously servers as a carbon source and a nitrogen source, so that obtained carbon quantum dots have high impurity and moderate particle sizes, are monodisperse and water soluble and are expected to be applied to the LED membrane materials, fluorescent marks, DNA carriers and targeted drug delivery particles.

The invention discloses a method for producing food-grade potassium chloride by adopting an adsorption process. The method comprises steps such as dissolving, filtering, multilevel macroporous resin adsorption purification, active carbon adsorption, concentration crystallization, solid-liquid separation, drying and the like. Octadecylamine, dodecylmorpholine and Number 2 oil in the industrial potassium chloride are removed by virtue of adsorption purification of macroporous resin and active carbon, so that the purposes of purifying the industrial potassium chloride and producing the food-grade potassium chloride can be realized.

A process for renaturating and purifying the inclusion body protein includes such steps as dissolving the inclusion body protein in acidic/alkaline flowing phase or other solution, loading it in the ion-exchange chromatographic column, reversible adsorbing it by the chromatographic medium, and modifier concentration gradient elution. Its advantages are high concentration and activity of recovered protein and high purity.

The invention provides a range hood provided with a multi-layer purifying and adsorbing device. The range hood comprises a dynamic centrifugal adsorbing device, an air channel condensing and adsorbing device and an electrostatic adsorbing device which are sequentially arranged from a cooking fume suction direction to a cooking fume exhaust direction; the dynamic centrifugal adsorbing device comprises a rotatable centrifugal mesh disc; the electrostatic adsorbing device can form a high-voltage electric field for adsorbing cooking fume particles; and the air channel condensing and adsorbing device is used for conveying cooking fume and condensing and adsorbing the cooking fume in a conveying process. According to the range hood provided by the invention, at least three layers of adsorbing devices are arranged for adsorbing and purifying the cooking fume particles, therefore the indexes such as air volume of the range hood reach the standards, the PM2.5 emission index is also reduced more effectively, and the overall environment of a kitchen and air is improved.

The invention discloses a purification method of polysilicon by-product silicon tetrachloride. The purification method comprises the following step: adsorbing the polysilicon by-product silicon tetrachloride by amphoteric ion exchange resin to obtain purified silicon tetrachloride. In the purification method, the amphoteric ion exchange resin is adopted, so that the impurity content can be reduced, and the purity of polysilicon by-product silicon tetrachloride is improved effectively. Meanwhile, the amphoteric ion exchange resin can be used stably for a long time, so that the amount of resin used during long-term operation can be reduced, and the treatment cost of waste resin after use can be lowered. The purification method is simple, is low in energy consumption, is low in cost, and is conducive to the reuse of silicon tetrachloride.

The invention provides a kit for extracting stable bacterial genome DNA by a paramagnetic particle method and an extraction method thereof. The kit comprises a lysis reagent group for lysing and releasing bacterial genome DNA and an extraction reagent group for extracting the genome DNA, wherein the lysis reagent group consists of a buffer solution and a lysis solution; the extraction reagent group consists of a paramagnetic particle binding solution, a washing solution and an eluent; and the paramagnetic particle binding solution consists of sodium salt sodium citrate or sodium acetate, a reducing agent dithiothreitol, guanidine hydrochloride and a paramagnetic particle suspension. The raw materials are reasonably compounded to obtain the optimal proportion and the combined extraction conditions of a paramagnetic particle binding solution compounding system, and the extraction method is optimized, so that the extracted bacterial genome DNA has high purity and complete fragments, is difficult to degrade, has stability far superior to that of the commercially available kit, and has remarkable advantages in subsequent experiments such as DNA storage, hybridization, PCR and the like; and meanwhile, the reagents are nontoxic and have small environmental pollution.

The invention relates to a method for purifying phosphogypsum by using hydrocyclone. The method comprises the following steps: mixing the phosphogypsum with water according to the weight part ratio of 5-40: 60-95, and then grinding the mixture into slurry; sieving the slurry obtained in the step A by using a square hole sieve with meshes of 40-100, and stirring the slurry capable of passing through the sieve in a stirring pond; pumping the slurry in the stirring pond in the hydrocyclone while stirring, and purifying and separating the slurry by the hydrocyclone under the condition that the pressure of a feeding opening of the hydrocyclone is 0.1-0.4 Mpa so that impurities which are separated out flow out from an overflowing opening of the hydrocyclone along with the water and enter a settling pond and the high-purity phosphogypsum obtained by purification flows out from a discharging opening of the hydrocyclone; and naturally filtering out water from the phosphogypsum slurry obtained by purification or centrifugally separating the phosphogypsum slurry to obtain the high-purity phosphogypsum. By the method, water consumption in a purification process can be reduced.

The invention discloses equipment and a method for preparing high-purity tellurium. The equipment comprises a vacuum distillation device, a vacuumizing device, a hydrogen purification circulating device, a high-frequency induction heating device and a product collecting device; the vacuumizing device is used for providing a vacuum environment and comprises a single-hole vacuum suction filter and adouble-hole vacuum suction filter, and the single-hole vacuum suction filter is connected with an extraction opening in a vacuum distillation kettle; and the hydrogen purification circulating deviceis used for providing a hydrogen reduction environment for the product collecting device. When the equipment is used for preparing high-purity tellurium, the process is short, the energy consumption is low, the tellurium remelting procedure in the conventional high-purity tellurium preparation process is omitted, and the problems of high energy consumption caused in the remelting process and localoxidation caused in the charging process are avoided.

The invention provides an application of a silica-gel chromatographic column to detection of high-purity gas via gas chromatography, and relates to a method and a device for detecting high-purity gas by using the silica-gel chromatographic column. The application of the silica-gel chromatographic column to detection of the high-purity gas has the characteristics that the perfect chromatographic peak shape is provided, high sensitivity and good selectivity are obtained, the sharp peak is realized, the peak capacity is high and the like.

The invention provides a method for separating and purifying ursolic acid from rosemary, wherein the method comprises the following steps: 1) taking dried rosemary leaves, carrying out reflux extraction on the dried rosemary leaves by an ethanol-water solution, and filtering to obtain a filtrate 1; concentrating the filtrate 1 under reduced pressure, adding water, and standing for cooling; filtering to obtain a filter cake 1, and stirring the filter cake 1 with an acid-ethanol-water solution; 2) filtering to obtain a filter cake 2, and carrying out reflux extraction on the filter cake 2 by analkali-ethanol-water solution; and filtering to obtain a filtrate 2, and adding activated carbon into the filtrate 2, and decolorizing; and 3) filtering to obtain a filtrate 3, adding an acid-water solution into the filtrate 3 until no precipitate appears, carrying out suction filtration to obtain a filter cake 3, and repeatedly crystallizing the filter cake 3 by an ethanol-water solution. According to the method, a pickling step is added in the separation and purification process by utilizing different dissolvability, impurities with polarity larger than that of ursolic acid and similar to that of ursolic acid can be effectively removed in the pickling step, a great purification effect is achieved on the subsequent acid precipitation step, the recrystallization frequency can be reduced, and high-purity ursolic acid is obtained.

The invention relates to the technical field of environmental protection and resource recovery, in particular to a method and device for recovering and purifying phosphorus from sludge incineration ash. The method comprises the following steps: acid extraction: pickling the sludge incineration ash, and conducting filtering to obtain an acid extract; and electrochemical purification: with the acidextract as an anolyte, a nitrate-containing salt solution as a catholyte and inert electrodes as an anode and a cathode, arranging a cation exchange membrane between the anolyte and the catholyte, andcarrying out an electrochemical reaction, wherein the anolyte after the electrochemical reaction is a purified phosphate radical solution. The method can realize efficient recovery and high purification of the phosphorus element in the sludge incineration ash, is high in operability, and has good industrial application prospects.

The invention relates to a dairy farm sewage treatment device and a treatment method thereof. The dairy farm sewage treatment device comprises a solid-liquid separation device, an osmotic membrane filtration device, an ultrasonic sterilization actuator and a waste storage tank; waste in the waste storage tank is conveyed to the solid-liquid separation device for solid-liquid separation, and wastewater mixture separated from the solid-liquid separation is conveyed to the osmotic membrane filtration device for filtration, and clear liquid from the osmotic membrane filtration device can be conveyed to the ultrasonic sterilizer actuator for sterilization; and the dairy farm sewage treatment device realizes the efficient separation and degradation of pollutants, and residual colon bacillus inthe clear liquid is treated through an ultra-high power ultrasonic, the sterilization rate reaches 100%, the whole treatment system is convenient to maintain, the treatment cost is lowered, and the operation and management are simple and convenient.

The invention belongs to the field of non-ferrous metal hydrometallurgy, and particularly relates to a method for efficiently recovering copper and tungsten from copper-tungsten alloy waste. The method comprises the following steps that the copper-tungsten alloy waste is crushed, mixed evenly with sodium hydroxide, sodium carbonate and borax, a small amount of water is further added, and the temperature is raised to 300-500 DEG C, so that the sodium hydroxide is in a molten state, hydrogen peroxide is dropped slowly, after the reaction is complete, a material is solidified, a proper amount ofhot water is added to dissolve the material, solid and liquid are separated, tungsten enters a liquid phase in the form of sodium tungstate, and copper remains in a solid phase in the form of the copper and copper oxide. Qualified sodium tungstate products are obtained by concentrated crystallization of the sodium tungstate, and the copper oxide is reduced by hydrogen to obtain copper powder. Thetechnical process is simple, the treatment cost is low, the recovery rate is high, and the copper-tungsten recovery rate is greater than 99%.

The invention discloses a method for measuring sesquiterpenoids in tobacco. The method for measuring sesquiterpenoids in tobacco comprises the following steps: using an organic solvent to extract a sample; purifying the sample through gel column chromatography; adopting gas chromatography mass spectrometry (GC-MS) to analyze the sample purified through gel column chromatography; and measuring the content of the sesquiterpenoids through selected ion monitoring. The invention further discloses application of the measuring method to evaluation of the tobacco style and the tobacco quality.

The invention discloses a method for comprehensively extracting saponin and polyphenol from Camellia oleifera Abel. cakes. The method comprises the following steps: (1) extracting the saponin and thepolyphenol in the Camellia oleifera Abel. cakes by utilizing an ethanol-water system; (2) filtering an extracting solution through a ceramic membrane; (3) after concentrating ceramic membrane permeated liquid, regulating the pH (Potential of Hydrogen); then adsorbing by adopting macroporous resin and eluting by utilizing water, an NaOH solution and an ethanol solution respectively; (4) regulatingthe pH of an NaOH eluting solution and concentrating by a nano-filtration membrane; drying to obtain the polyphenol; (5) concentrating an ethanol eluting solution until no alcohol flavor is formed, and drying to obtain the saponin. According to the method disclosed by the invention, a membrane separation and macroporous resin adsorption technology is adopted, and the influences of macromolecular compounds including polysaccharides, protein, grease, pigments and the like, and impurities including salt and the like in a traditional method on saponin and polyphenol separation and purification areovercome; the method has the characteristics of simple equipment operation, moderate conditions, easiness for controlling, simple process route, high product purity and the like.

The invention belongs to the technical field of recombinant protein separation and purification, and mainly relates to a method for separating and purifying beta-glucosidase fusion protein, in particular to a method for constructing a foam separation system by using hydrophobic label performance and separating and purifying beta-Glu fusion protein by combining the temperature-sensitive performance of fusion enzyme. The invention aims to overcome the defects of complex extraction process, low separation and purification efficiency, high cost and the like in the existing protein separation and purification method, and provides a process for separating and purifying beta-glucosidase fusion protein by combining foam separation and inverse transition cycling (ITC). The process has the advantages of simple equipment, low investment, low energy consumption, no pollution and the like, is good in separation effect and low in enzyme activity loss, and is of great practical significance.