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Method for measuring sesquiterpenoids in tobacco

A determination method and technology for sesquiterpenes are applied in the field of analytical chemistry to achieve the effects of good effect, good precision and wide linear range

Active Publication Date: 2017-01-04
CHINA TOBACCO YUNNAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

We have established a method for the determination of sesquiterpenoids, an important sesquiterpenoid component in tobacco, by gel purification of samples and GC-MS analysis. At present, this method has not been reported in relevant literature

Method used

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  • Method for measuring sesquiterpenoids in tobacco
  • Method for measuring sesquiterpenoids in tobacco
  • Method for measuring sesquiterpenoids in tobacco

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Select flue-cured tobacco leaves, accurately weigh 1.0 g of the sample, add 20 mL of ethyl acetate to reflux for extraction for 20 min, concentrate the extract to 0.5 mL, add it to the packed gel column (the material is dextran gel, the gel column The packing specification is 0.6×150mm, and the packing solvent is ethyl acetate), the gel column loaded with samples is eluted with ethyl acetate, and the eluted fractions of 10 to 11 times the column volume are collected and then concentrated to 0.5mL; The chromatographic conditions and mass spectrometry conditions determined that the content of the sesquiterpenoids sesquiterpenoids was 6.26 μg / g.

Embodiment 2

[0034] Select oriental tobacco leaves, accurately weigh 0.50 g of the sample, add 20 mL of acetone to reflux for extraction for 20 min, concentrate the extract to 0.5 mL, add it to the packed gel column (the material is dextran gel, the gel column is filled The specification is 0.6×150mm, the column solvent is acetone), the gel column loaded with samples is eluted with acetone, and the eluted fractions of 10 to 11 times the column volume are collected and then concentrated to 0.5mL; according to the selected chromatographic conditions and mass spectrometry Conditional measurement, the content of sesquiterpenoids sesquiterpenoids is 11.2 μg / g.

Embodiment 3

[0036] Select the sample as Baijia tobacco leaves, accurately weigh 1.0g of the sample, add 20mL of ethanol for reflux extraction for 20min, concentrate the extract to 0.5mL, and add it to the packed gel column (the material is dextran gel, gel The packing specification of the column is 0.6×150mm, and the packing solvent is ethanol), and the gel column loaded with the sample is eluted with ethanol, and the eluted fraction of 10 to 11 times the column volume is collected and then concentrated to 0.5mL; according to the selected chromatographic Conditions and mass spectrometry conditions determined that the content of sesquiterpenoids sesquiterpenoids was 4.26 μg / g.

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Abstract

The invention discloses a method for measuring sesquiterpenoids in tobacco. The method for measuring sesquiterpenoids in tobacco comprises the following steps: using an organic solvent to extract a sample; purifying the sample through gel column chromatography; adopting gas chromatography mass spectrometry (GC-MS) to analyze the sample purified through gel column chromatography; and measuring the content of the sesquiterpenoids through selected ion monitoring. The invention further discloses application of the measuring method to evaluation of the tobacco style and the tobacco quality.

Description

technical field [0001] The invention belongs to the technical field of analytical chemistry, in particular to a method for determining sesquiterpenoids in tobacco. Background technique [0002] Smoking is an indispensable part of the cultural life of hundreds of millions of smokers, and it is also an important source of national and local taxes. Although smoking is widely recognized as harmful to health, tobacco still has a strong appeal to thousands of consumers. In addition to the addictive properties of nicotine, the rich flavor substances in tobacco also play an important role. [0003] The aroma components in tobacco have always been a research hotspot at home and abroad. The research on tobacco aroma components abroad was mainly concentrated in the 1970s. Swedish scholar Hlubucek J.R and others used phytochemical methods to separate a series of new compounds from tobacco. These research results A series of research papers have been published in "Acta Chemica Scandinav...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 杨光宇申钦鹏刘春波唐石云刘志华姚守拙姜瑞石倩倩张凤梅司晓喜何沛缪明明
Owner CHINA TOBACCO YUNNAN IND
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