A kind of capsicum genetic transformation method
A genetic transformation method and pepper technology, applied in the field of pepper genetic transformation, can solve problems such as difficulties in transgenic plants, and achieve the effects of batch operation genotype dependence, high transformation rate, and solving difficulties in genetic transformation.
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Embodiment 1
[0044] (1) Sowing and raising seedlings: Sow 2 seeds of Xiangtan chibanjiao (8214) in each hole, cover the hole tray with a 45cm wide plastic wrap, fix the plastic wrap with transparent tape, put it in a hydroponic box for 2 hours, and fully absorb it Cover the seeds with an appropriate amount of substrate after the nutrient solution; cut a 0.8-1.0 cm long opening in the middle of the hole with a blade after 6 days to ensure that the seeds and pepper seedlings are well ventilated; The grown seedlings are pinched out with tweezers to make them grow upright;
[0045] (2) Activation of Agrobacterium: On a sterile operating table, use an inoculation loop to dip a small amount of Agrobacterium transferred into the PFGC5941 Bar gene, and draw a plate on the YEP solid medium. After 2 days, under sterile conditions, dip a single colony on the 1L YEP liquid medium, at 28°C and in the dark, shake the bacteria for 3 days to OD 600 =0.6~1.0, resuspend with 30g / L sucrose solution after ce...
Embodiment 2
[0051] (1) Sowing and raising seedlings: Sow 2 seeds of Xiangtan chibanjiao (8214) in each hole, cover the hole tray with a 45cm wide plastic wrap, fix the plastic wrap with transparent tape, put it in a hydroponic box for 2 hours, and fully absorb it Cover the seeds with an appropriate amount of substrate after the nutrient solution; cut a 0.8-1.0 cm long opening in the middle of the hole with a blade after 6 days to ensure that the seeds and pepper seedlings are well ventilated; The grown seedlings are pinched out with tweezers to make them grow upright;
[0052] (2) Activation of Agrobacterium: On a sterile operating table, use an inoculation loop to dip a small amount of Agrobacterium transferred into the PFGC5941Bar gene, and draw a plate on the YEP solid medium. After 2 days, under sterile conditions, dip a single colony on the 1L YEP liquid medium, at 28°C and in the dark, shake the bacteria for 3 days to OD 600 =0.6~1.0, resuspend with 30g / L sucrose solution after cen...
Embodiment 3
[0058] (1) Sowing and raising seedlings: Sow 2 seeds of Xiangtan chibanjiao (8214) in each hole, cover the hole tray with a 45cm wide plastic wrap, fix the plastic wrap with transparent tape, put it in a hydroponic box for 2 hours, and fully absorb it Cover the seeds with an appropriate amount of substrate after the nutrient solution; cut a 0.8-1.0 cm long opening in the middle of the hole with a blade after 6 days to ensure that the seeds and pepper seedlings are well ventilated; The grown seedlings are pinched out with tweezers to make them grow upright;
[0059] (2) Activation of Agrobacterium: On a sterile operating table, use an inoculation loop to dip a small amount of Agrobacterium transferred into the PFGC5941 Bar gene, and draw a plate on the YEP solid medium. After 2 days, under sterile conditions, dip a single colony on the 1L YEP liquid medium, at 28°C and in the dark, shake the bacteria for 3 days to OD 600 =0.6~1.0, resuspend with 30g / L sucrose solution after ce...
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