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A kind of yeast strain rich in ergosterol and the preparation method of ergot yeast powder

A yeast strain and ergosterol technology, which is applied in the field of fermentation engineering, can solve the problems of unstable fermentation ability of strains, low ergosterol content, and inability to guarantee ergosterol production, so as to improve nutrient absorption capacity, high ergosterol content, prevent churn effect

Active Publication Date: 2019-07-19
GUANGDONG WUZHOU PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of the low content of ergosterol produced by yeast at present, the fermentation ability of the strain is unstable, and the production of ergosterol cannot be guaranteed

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1, a kind of preparation method of ergot yeast powder

[0035] Preparation of S1 acidified solution: Take drinking water and mix it with molasses, then heat it to 100°C with steam, adjust the sugar brix to 20Bx, let it settle for 4 hours, filter the ash, take the supernatant to obtain the acidified solution, the acidified solution The weight ratio of sugar content is 20%, the pH value is 3.6, and the Brix is ​​27Bx;

[0036] Preparation of S2 nutrient solution: mix 8 parts of ammonium sulfate, 8 parts of ammonium dihydrogen phosphate, 2 parts of phosphoric acid, 2 parts of magnesium sulfate, 2 parts of potassium sulfate and 4 parts of urea, add 20 parts of drinking water to dissolve, and obtain the nutrient solution. The pH value in the nutrient solution is 1.2, and the Brix is ​​12.4;

[0037] S3 strain activation: Saccharomyces cerevisiae was inoculated in a slant medium containing 4Bx wort and 1% agar, and cultured for 26 hours at a constant temperature o...

Embodiment 2

[0042] Embodiment 2, a kind of preparation method of ergot yeast powder

[0043] Preparation of S1 acidified solution: Take drinking water and mix it with molasses, then heat it to 110°C with steam, adjust the sugar brix to 25Bx, let it settle for 6 hours, filter the ash, take the supernatant to obtain the acidified solution, the acidified solution The weight ratio sugar content is 22%, the pH value is 4.2, and the Brix is ​​30Bx;

[0044] Preparation of S2 nutrient solution: Mix 10 parts of ammonium sulfate, 10 parts of ammonium dihydrogen phosphate, 3 parts of phosphoric acid, 3 parts of magnesium sulfate, 3 parts of potassium sulfate and 5 parts of urea, add 50 parts of drinking water to dissolve, and obtain the nutrient solution. The pH value in the nutrient solution is 2.0, and the Brix is ​​18.4;

[0045] S3 strain activation: Saccharomyces cerevisiae was inoculated in a slant medium containing wort juice of 6Bx and 2% agar, cultivated for 24 hours at a constant tempera...

Embodiment 3

[0050] Embodiment 3, a kind of preparation method of ergot yeast powder

[0051] Preparation of S1 acidified solution: Take drinking water and mix it with molasses, then heat it to 120°C with steam, adjust the sugar brix to 30Bx, let it settle for 8 hours, filter the ash, take the supernatant to obtain the acidified solution, the acidified solution The weight ratio sugar content is 25%, the pH value is 5.0, and the Brix is ​​36Bx;

[0052] Preparation of S2 nutrient solution: Mix 12 parts of ammonium sulfate, 12 parts of ammonium dihydrogen phosphate, 4 parts of phosphoric acid, 4 parts of magnesium sulfate, 4 parts of potassium sulfate and 6 parts of urea, add 60 parts of drinking water to dissolve, and obtain the nutrient solution. The pH value in the nutrient solution is 2.5, and the Brix is ​​22.6;

[0053] S3 strain activation: Saccharomyces cerevisiae was inoculated in a slant medium containing 8Bx wort and 3% agar, and cultured for 20 h at a constant temperature of 32°...

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Abstract

The invention belongs to the technical field of fermentation engineering, and in particular relates to a yeast strain rich in ergosterol and a preparation method of ergot yeast powder. The yeast strain rich in ergosterol provided by the invention has the advantages of high ergosterol content, high fermentation efficiency and strong fermentation ability. The ergot yeast powder dry matter and ergosterol content prepared by adopting the ergosterol-rich yeast strain provided by the invention are high, and its dry matter is greater than 1.26g / 100ml, and the ergosterol content is greater than 1.62%. The preparation process of ergot yeast powder by the yeast strain rich in ergosterol is simple and low in cost, and is beneficial to the industrial production of the ergot yeast powder.

Description

technical field [0001] The invention belongs to the technical field of fermentation engineering, and in particular relates to a yeast strain rich in ergosterol and a preparation method of ergot yeast powder. Background technique [0002] The chemical name of ergosterol is 24β-methylcholesterol-5,7ene, 3β-hydroxyl, and the molecular formula is C 28 h 44 O, with a molecular weight of 396.66, is an important pharmaceutical and chemical raw material and a pesticide raw material. Ergosterol can be used in the production of drugs such as "cortisone" and "hormone progesterone", and can also be used to vigorously produce brassinolide and plant auxin. At the same time, ergosterol can also enhance the body's ability to resist diseases, and vitamin D can be obtained after proper ultraviolet irradiation. 2 , vitamin D 2 It has an important function in regulating the metabolism of human body and animals, and has the effect of promoting the absorption of calcium, phosphorus and other ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/18C12R1/865
CPCC12N1/18C12N1/185C12R2001/865
Inventor 叶志励陈大标张才军谢潮山
Owner GUANGDONG WUZHOU PHARMA
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