A kind of clean production process of L-glutamine fermentation method

Abstract

The invention belongs to the technical field of separation and extraction of amino acid and discloses a clean production process of L-glutamine by adopting a fermentation method. The clean production process comprises the steps of filtering, flocculating, performing isoelectric operation, concentrating, performing ultrafiltration, drying and the like, and finally, an L-glutamine product is obtained. The clean production process disclosed by the invention is operated according to the steps, so that the yield and the purity of the L-glutamine product are improved, the problem about environment caused by fermented industrial wastewater and waste residues is effectively solved, clean production of the L-glutamine by adopting the fermentation method is realized, and two purposes by one process are achieved.

Description

technical field

[0001] The invention belongs to the technical field of separation and extraction of amino acids, and in particular relates to a clean production process of L-glutamine fermentation method. Background technique

[0002] L-glutamine is the most common and indispensable conditional essential amino acid in the human body, accounting for 61% of the free amino acids in the human body. Originally found in beet juice, sprouted seeds and protein. L-glutamine plays a very important role in life activities. It is an amino acid that constitutes protein and an important biochemical reagent. It is mainly used clinically to treat diseases such as alcoholism, depression, and peptic ulcer, and has broad prospects for drug development.

[0003] Affected by the characteristics of the industry, fermented products need to consume more water, electricity, steam, etc. in the production process. Waste water and waste residues in the fermentation industry are rich in protein, amino...

Examples

Embodiment 1

[0022] A kind of L-glutamine fermentation clean production process, it comprises the steps:

[0023] Step 1) Prepare flocculant:

[0024] (1) Add purified water accounting for twice the mass of starch to the starch, stir evenly to obtain a suspension, then add sodium carboxymethyl cellulose with the same mass as the starch, stir at 200 rpm for 30 minutes, and then dry at 80°C Dry to less than 5% (mass percentage) of moisture content, obtain modified starch;

[0025] (2) Take chitosan, sodium humate and pectin according to the mass ratio of 5:1:0.2, mix and stir evenly, then add 2M aqueous sodium hydroxide solution accounting for twice the mass of chitosan, and ultrasonic 10min at 30KHz, Last 500 revs / min stirs 3min, obtains modified chitosan;

[0026] (3) Pulverize corn stalk powder and sepiolite respectively to obtain corn stalk powder and sepiolite powder, mix and stir corn stalk powder, sepiolite powder and diatomite according to the mass ratio of 3:1:1, add to 5 times t...

technical field

[0001] The invention belongs to the technical field of separation and extraction of amino acids, and in particular relates to a clean production process of L-glutamine fermentation method. Background technique

[0002] L-glutamine is the most common and indispensable conditional essential amino acid in the human body, accounting for 61% of the free amino acids in the human body. Originally found in beet juice, sprouted seeds and protein. L-glutamine plays a very important role in life activities. It is an amino acid that constitutes protein and an important biochemical reagent. It is mainly used clinically to treat diseases such as alcoholism, depression, and peptic ulcer, and has broad prospects for drug development.

[0003] Affected by the characteristics of the industry, fermented products need to consume more water, electricity, steam, etc. in the production process. Waste water and waste residues in the fermentation industry are rich in protein, amino...

Examples

Embodiment 1

[0022] A kind of L-glutamine fermentation clean production process, it comprises the steps:

[0023] Step 1) Prepare flocculant:

[0024] (1) Add purified water accounting for twice the mass of starch to the starch, stir evenly to obtain a suspension, then add sodium carboxymethyl cellulose with the same mass as the starch, stir at 200 rpm for 30 minutes, and then dry at 80°C Dry to less than 5% (mass percentage) of moisture content, obtain modified starch;

[0025] (2) Take chitosan, sodium humate and pectin according to the mass ratio of 5:1:0.2, mix and stir evenly, then add 2M aqueous sodium hydroxide solution accounting for twice the mass of chitosan, and ultrasonic 10min at 30KHz, Last 500 revs / min stirs 3min, obtains modified chitosan;

[0026] (3) Pulverize corn stalk powder and sepiolite respectively to obtain corn stalk powder and sepiolite powder, mix and stir corn stalk powder, sepiolite powder and diatomite according to the mass ratio of 3:1:1, add to 5 times t...