41results about How to "The extraction process is environmentally friendly" patented technology

The invention discloses a preparation method of a fish collagen combined chitosan biological dressing. The preparation method comprises the following steps: cutting clean fish wastes, from which surface impurities are removed, into fish meal of 50-200 meshes; putting the fish meal in an airtight container and then adding distilled water and a reaction enzyme into the airtight container, and stirring to form slurry; reacting the slurry under high pressure and ultrasonic treatment; after the reaction is finished, subjecting the slurry to purification treatment; adding chitosan to the purified slurry, heating until the temperature is in a range from 80 DEG C to 90 DEG C and stirring for 20-30 minutes; and finally, carrying out dialysis, concentration and drying, thereby obtaining the fish collagen combined chitosan biological dressing. The preparation method disclosed by the invention is simple in process, easy to implement, convenient to operate, and low in cost; the quickly extracted fish collagen is even in molecular weight distribution and few in impurities; the prepared biological dressing is good in mechanical strength, and has the effects of stopping bleeding and promoting wound healing, and also has the effect of antibiosis.

The invention discloses a glutamic acid separating and extracting technology. The technology is characterized in that glutamic acid in the isoelectric point liquid of glutamic acid is separated and extracted through adopting an ion exchange method, an acid solution not an alkali solution is directly used to elute for generation after the ion exchange resin adsorption of the glutamic acid, and an eluate obtained after the ion exchange returns to an isoelectric operation for glutamic acid extraction. Compared with traditional technologies, the technology disclosed in the invention has the advantages of saving of a large amount of an acid and an alkali, reduction of the production cost and the environmental pollution, and economic benefit improvement.

The invention provides a method for smelting high-purity nickel-molybdenum alloy by using complex nickel-molybdenum ore. The alloy comprises the following components in percentage by mass: 8 to 30 percent of Ni, 10 to 30 percent of Mo, less than or equal to 0.5 percent of Si, less than or equal to 0.5 percent of C, less than or equal to 0.1 percent of Cu, less than or equal to 0.03 percent of P, less than or equal to 0.03 percent of S, and the balance of iron. According to the characteristic that the complex nickel-molybdenum ore is difficult to separate, the high-purity nickel-molybdenum alloy is smelted by adopting the routes of oxidizing and roasting nickel-molybdenum sulfide type mineral to remove sulfur, reducing and smelting high-alkalinity slag to obtain crude alloy, refining high-alkalinity weak oxidative slag to remove silicon and phosphorus and treating and refining Ba-Ca in vacuum to deeply remove phosphorus, sulfur and oxygen; the alloy can be used for nickel-molybdenum alloying and alloying of high nickel-molybdenum alloy steel in high-purity steel production, and has the advantages of high purity, no pollution, good alloying effect and the like; and the method has the characteristics of pure fire method, greenness, no pollution, simplicity in process and the like, realizes efficient utilization of the difficultly separated complex nickel-molybdenum ore, and is worthy of popularization.

The invention provides a preparation method and an application of a camellia flower extracting solution. The method comprises steps as follows: A, selecting fresh camellia flowers without damage and diseases, and drying the camellia flowers with a refraction window drying technology to obtain a dried camellia flower product; B, performing airflow superfine crushing and sieving on the dried camellia flower product, and adding a quaternary system deep-eutectic solvent for ultrasonic extraction to obtain an extracted mixture; C, mixing the extracted mixture and deionized water, regulating pH, andadding enzyme for enzymolysis; D, leaving a product to stand for a certain time after enzymolysis, and filtering the product to obtain the camellia flower extracting solution. According to the method, drying is performed with the refraction window drying technology, superfine crushing is performed, the non-toxic deep-eutectic solvent is taken as an extracting agent, and the production process issimple, efficient, safe and green; the defects that a large quantity of toxic organic solvents and complicated column separation processes are required for production of conventional epicatechin are overcome. The obtained extracting solution has the skin repairing efficacy, the bacteriostasis function and high antioxidation capability and is safe and non-irritant.

The invention discloses a method for rapidly extracting fish collagen protein by using an enzymolysis method. Degreasing treatment and protein extraction are accomplished in one step by using a high pressure ultrasonic enzymolysis agent, the extraction efficiency is greatly improved, and the protection time is shortened. The extraction process is environment-friendly, rapid and convenient, uniform in fish collagen protein molecular weight distribution, and small in impurity.

The invention provides a method for extracting yak skin collagen by adopting an enzyme process and relates to the field of collagen extraction. The method comprises the following steps of unhairing, smashing, degreasing, enzymolysis and purification. The method for extracting the yak skin collagen by adopting the enzyme process has the advantages that yak skin is used as a raw material, and the method aims at obtaining collagen excellent in performance and improving the product value and economic benefit of the yak skin. The collagen prepared by utilizing hydrolysis of an enzymic preparation after unhairing and degreasing is high in purity, uniform in color and luster and free of extraneous odor, the extraction process is green and environment-friendly, the process is simple, a yak skin utilization range can be widened, and a yak skin utilization value can be increased.

The invention discloses an electronic cigarette liquid rich in biological active polysaccharide and a preparation method of the electronic cigarette liquid. The electronic cigarette liquid is mainly formed by the following raw materials in parts by weight: 50-70 parts of propylene glycol, 5-15 parts of glycerin, 5-10 parts of deionized water, 5-15 parts of natural biological active polysaccharide extracts, 3-10 parts of tobacco extracts, 1-5 parts of tobacco flavor, 1-3 parts of thickener and 1-3 parts of antioxidant. The electronic cigarette liquid disclosed by the invention is suitable for being smoked in an electronic atomizing way and is formed by refining the tobacco extracts and the natural biological active polysaccharide extracts, an immune function of a human body is also widely regulated while the feeling of smoking a cigarette is obtained by a consumer, and a certain healthcare function is obtained.

The invention discloses an extraction method of polysaccharide with activities of kidney warming and Yang tonifying from Aspongopus chinensis Dallas insects of Pentatomidae and application of the polysaccharide. The extraction method comprises the following steps: (1) drying Aspongopus chinensis Dallas insect bodies, performing pulverization, performing reflux extraction with petroleum ether, andconducting filtration liquid-solid separation so as to obtain drug residues with removal of grease; (2) evaporating the drug residues, performing reflux extraction with distilled water, performing filtration, combining the filtrate, and performing concentration so as to obtain total water extract; (3) adding distilled water to the total water extract for slight dilution, performing precipitation on polysaccharide with an alcohol solution, performing stirring while the alcohol solution is added, and performing still standing and filtration so as to obtain an insect polysaccharide alcohol precipitate; and (4) adding water to the insect polysaccharide alcohol precipitate for dilution and dissolution, performing chromatography separation and purification by using a DEAE-Sephadex ged cellulosecolumn, and performing rinsing with distilled water and a sodium chloride solution so as to obtain the Aspongopus chinensis Dallas polysaccharide. The invention also relates to the application of thepolysaccharide in preparation of drugs for treating or preventing Yang deficiency, impotence and erectile dysfunctions, the method is easy has simple and convenient operation, and the obtained polysaccharide compound with a purity of up to 95% has strong effects of kidney warming, Yang tonifying, fatigue resistance and body immunity recovery.

The invention discloses guaiazulene and an extraction process thereof. The extraction process comprises the following steps: distilling and vacuum collecting natural essence and fragrance oil comprising an azulene compound, and obtaining a fraction A; dehydrating the fraction A, decompression distilling, and obtaining a fraction B; dehydrogenating the fraction B, vacuum distilling, and obtaining an azulene-containing mixed solution; adding an acid solution B and organic solvent into the azulene-containing mixed solution, uniformly mixing, standing, layering, uniformly mixing and stirring the acid layer solution, cold water A and petroleum ether, settling, discharging the acid water, filtering the residues, placing the residual solution into a recycling kettle, adding cold water B into therecycling kettle, slowly heating until all petroleum ether is completely distilled, cooling, standing, layering, removing the moisture, and obtaining a guaiazulene crude product; and crystallizing andfiltering the guaiazulene crude product, and obtaining a guaiazulene pure product with the purity being 99 percent or above and the yield of 2.9 to 3.6 percent. The extraction process is safe, and environmentally friendly, and prepared guaiazulene is high in purity and high in yield.

The invention provides a preparation method of an animal blood active complex peptide. The method combines a membrane separation technology and a biologically directional enzyme digestion technology,can effectively protect animal serum protein and polypeptide activities, and can be used for preparing high-activity animal blood active peptide. According to the method, steps including chemical or high-temperature deformation, spray-drying and the like in a traditional animal blood polypeptide extraction process are removed, a grade-by grade extraction mode is adopted, in the same technical process, an active peptide A (plasma ultrafiltration peptide) contained in plasma is first extracted with a 5000Da ultrafiltration membrane, a plasma protein peptide B (plasma protease digestion peptide)is extracted by using plasma subjected to ultrafiltration and concentration as a raw material and conducting direct enzyme digestion, ultrafiltration and reverse osmosis concentration, an active polypeptide complex formed by combining the active peptide A and the plasma protein peptide B is rich and comprehensive in types, and the two active polypeptides can achieve a biological synergistic effectsince the two active polypeptides are complementary after being mixed. Compared with a relatively single animal polypeptide extracted by a traditional process, the active complex peptide has the advantage of stronger effects in the fields of medical treatment, cosmetology, health protection and the like.

The invention discloses a method for extracting antioxidant ingredients from an eggplant and a product. The method for extracting antioxidant ingredients from the eggplant comprises the following steps of: cutting the fresh eggplant into pieces; adding 0-30vol% ethanol pulp to the eggplant pieces; centrifuging the serous fluid; penetrating liquid supernatant through a non-polar macroporous resin column, washing the liquid supernatant until the liquid supernatant has no sugar; eluting the liquid supernatant by 40%-100vol% ethanol with volume 3-20 times that of the resin; concentrating and drying the eluant to obtain the eggplant antioxidant extract. The method disclosed by the invention is environment-friendly in extracting process and low in energy consumption; the total antioxidant activity of the extract is equivalent to that of ascorbic acid, and therefore, the method can be extensively applied to the fields including the food field, the medicine and health product field and the like.

The invention belongs to the technical field of separation and extraction of amino acid and discloses a clean production process of L-glutamine by adopting a fermentation method. The clean production process comprises the steps of filtering, flocculating, performing isoelectric operation, concentrating, performing ultrafiltration, drying and the like, and finally, an L-glutamine product is obtained. The clean production process disclosed by the invention is operated according to the steps, so that the yield and the purity of the L-glutamine product are improved, the problem about environment caused by fermented industrial wastewater and waste residues is effectively solved, clean production of the L-glutamine by adopting the fermentation method is realized, and two purposes by one process are achieved.

The invention relates to a method for preparing chestnut shell vegetable dye through a subcritical method and a dyeing method thereof, the preparation method comprises the following steps: 1) adding chestnut shells and purified water into subcritical stainless steel equipment, adding a high-temperature alkaline buffer agent AH into the subcritical stainless steel equipment, then heating to subcritical temperature, extracting chestnut shell natural pigment, filtering and discharging; and 2) adding a natural dyeing auxiliary agent FA into the extracting solution obtained after filtration in the step 1) to prepare the chestnut shell vegetable dye. The chestnut shell vegetable dye prepared by adopting a subcritical method is subjected to a one-bath low-temperature sectional dyeing method, so that higher dye-uptake and color fastness can be obtained, agricultural and forestry waste resources can be recycled, the environment is improved, and textiles dyed by plants are safer and more environment-friendly.

The invention belongs to the technical field of microbial polysaccharide production and discloses a hyaluronic acid fermentation liquor extraction method. The method includes the first step of obtaining hyaluronic acid filter liquor A, the second step of conducting decoloring, impurity removal and precipitation, the third step of conducting dissolving and complexing, the fourth step of conducting dissociation and filtering, and the fifth step of carrying out precipitation, dewatering and drying. The extraction method has the advantages of being high in extraction yield and product purity, little in environment pollution and the like.

The invention discloses a preparation method of active macromolecular keratin and application thereof as a biological dressing. According to the preparation method, macromolecular keratin with biological activity is extracted from waste wool by controlling the reaction conditions of keratinase, the molecular weight of the macromolecular keratin is mainly distributed between 45kDa and 28kDa, and the keratin can effectively promote proliferation and migration of fibroblasts and shows obvious oxidation resistance. The regenerated keratin powder can be self-assembled into hydrogel, can induce tissue regeneration and repair, effectively promotes wound healing, and is a good medical biological dressing. The method is simple in operation process, low in equipment cost and easy for industrial production; fibers, which cannot be used for textile processing, such as waste wool, cashmere, wool staple fibers, and the like, in the animal husbandry and the textile industry are used as raw materials,resources are saved, and the environment is protected. Keratinase is used for biological extraction, the extraction process is environmentally friendly, and pollution is avoided.

The invention discloses a method for extracting tricin from corn bracts. The method includes the steps that the corn bracts are subjected to normal temperature extraction with water, and extraction residues are obtained; an ethanol solution with the volume fraction of 80% to 90% is used for conducting reflux extraction on the extraction residues, an acid medium is added to the ethanol solution, an obtained extracting solution is subjected to vacuum concentration, and concentrate is obtained; the concentrate is diluted with water, the pH value is adjusted to 7.8 to 8.2, and supernatant is taken after solid-liquid separation; macroreticular resin column chromatography is conducted on the supernatant, and corn bract extract containing tricin is obtained. Normal temperature extraction is firstly conducted with water during alcohol extraction, and therefore protein, polysaccharide and other impurities can be removed in advance. The acid medium is added to the ethanol solution used for reflux extraction, and therefore the extraction efficiency of low-polarity tricin can be effectively improved. The pH value of the concentrate diluent is adjusted to 7.8 to 8.2, at the moment, chlorophyll, protein, polysaccharide and other impurities in the concentrate diluent quickly settle in the weak base environment, and the extraction period of tricin is greatly shortened.

The invention discloses a method for extracting podocar-pusflavone from juniper berry meal. The method comprises the steps that reflux extraction is performed on the juniper berry meal through an ethanol solution with the volume fraction of 70%-80% to obtain an extracting solution, and the extracting solution is concentrated under reduced pressure to obtain concentrate; the concentrate is diluted by adding water, solid-liquid separation is performed after standing is performed, and supernatant liquor is taken; the ethanol degree of the supernatant liquor is regulated to be 20%-30% through ethanol, solid-liquid separation is performed after standing is performed, and precipitates are taken; the precipitates are dissolved with a diluted hydrochloric acid solution and then added into a macroporous resin column, elution is performed with water until the pH value of effluent is neutral, then desorption is performed with the ethanol solution, a desorption solution is collected, and the desorption solution is concentrated under reduced pressure and dried to obtain a juniper berry meal extract. According to the method, the steps are simple and convenient, operation is easy, and the method is suitable for industrialized production; due to the fact that only water and ethanol are adopted as the solvent, not only is the cost greatly reduced, but also no stimulation or toxicity is basically generated to operating personnel and the environment; the content of podocar-pusflavone in the juniper berry meal extract reaches up to 30%-40%, and the extraction efficiency is high.

The invention discloses a method for extracting podocar-pusflavone from juniper berry meal. The method comprises the steps that reflux extraction is performed on the juniper berry meal through an ethanol solution with the volume fraction of 70%-80% to obtain an extracting solution, and the extracting solution is concentrated under reduced pressure to obtain concentrate; the concentrate is diluted by adding water, solid-liquid separation is performed after standing is performed, and supernatant liquor is taken; the ethanol degree of the supernatant liquor is regulated to be 20%-30% through ethanol, solid-liquid separation is performed after standing is performed, and precipitates are taken; the precipitates are dissolved with a diluted hydrochloric acid solution and then added into a macroporous resin column, elution is performed with water until the pH value of effluent is neutral, then desorption is performed with the ethanol solution, a desorption solution is collected, and the desorption solution is concentrated under reduced pressure and dried to obtain a juniper berry meal extract. According to the method, the steps are simple and convenient, operation is easy, and the method is suitable for industrialized production; due to the fact that only water and ethanol are adopted as the solvent, not only is the cost greatly reduced, but also no stimulation or toxicity is basically generated to operating personnel and the environment; the content of podocar-pusflavone in the juniper berry meal extract reaches up to 30%-40%, and the extraction efficiency is high.

The invention discloses a sun-resistant gelata prepared from oyster polysaccharide. The sun-resistant gelata is characterized by comprising the following raw materials in parts by weight: 2-5 parts of oyster polysaccharide, 0.1-1 part of goldthread polysaccharide, 0.01-0.05 part of azone, 50-80 parts of glycerinum, 50-80 parts of 1, 2-propylene glycol, 60-90 parts of poloxamer 407 and 100-400 parts of water. The sun-resistant gelata prepared from oyster polysaccharide, disclosed by the invention, has sun-resistant and moisturizing functions, is sun-resistant and protects the skin simultaneously, eliminates free radicals, and promotes cellular regeneration of the skin.

The invention discloses a wound dressing of bacterial cellulose based sericin and peppermint oil nanoparticles and a preparation method of the wound dressing. The wound dressing is in a composite film form, and the composite film is composed of bacterial cellulose, sericin and peppermint oil; the bacterial cellulose is a 3D reticular biological high-molecular polymer synthesized through microbial fermentation, has good air permeability, water permeability and water retention performance, is excellent in mechanical property, and meets the basic requirements of wound dressings. Sericin is a natural hydrophilic protein in silkworm cocoons, has the potential of becoming a novel wound dressing, has cell protection and mitosis effects on fibroblasts and keratinocytes, can promote fiber proliferation, and enables the fibers to be attractive to skin development; and besides, the sericin has good hydrophilicity, biocompatibility, biodegradability and good affinity to biomolecules, so that the excellent moisture retention of the sericin is beneficial to accelerating wound healing.

The invention provides a method for preparing a camellia extract and its application. The method comprises the following steps: A. selecting fresh, non-damaged and disease-free camellias, and drying them by adopting a refraction window drying technique to obtain dried camellia products; B. After the dried product of camellia is ultrafinely pulverized and sieved by airflow, it is added into a quaternary system deep eutectic solvent for ultrasonic extraction to obtain an extraction mixture; C. Mix the extraction mixture with deionized water, adjust the pH, and then add enzymes for enzyme solution; D, after enzymolysis, place it for a certain period of time, and then filter to obtain the camellia extract. The method adopts the refraction window drying technology for drying, ultrafine pulverization, and uses non-toxic deep eutectic solvent as the extraction agent. The production process is simple, efficient, safe and green; it overcomes the need for a large amount of toxic organic solvents and Complex column separation process. The obtained extract has skin repairing effect, antibacterial effect, strong antioxidant capacity and is safe and non-irritating.

A method for extracting fructus momordicae tetranuclear-triterpene is carried out by leaching flavine from fresh fructus momordicae skin by warm water, heating gelatinous protein in fruit cell, solidifying, crushing, leaching by 60-90 deg. C, de-bonding by pectase, filtering, concentrating and drying to obtain final product. Crude products containing tetranuclear-triterpene 43% 300-350g are extracted from fresh fruits 10000g. It's pure, safe, efficient, simple and cheap. It has no environmental pollution. The pumace can be made into feed.

The invention discloses a bacterial cellulose-based wound dressing of sericin and peppermint oil nanoparticles and a preparation method thereof. The wound dressing is in the form of a composite film, and the composite film is composed of bacterial cellulose, sericin and peppermint oil; bacterial cellulose It is a 3D mesh biopolymer synthesized by microbial fermentation. It has good air permeability, water permeability and water holding performance, and excellent mechanical properties, which meets the basic requirements of wound dressings. Sericin is a natural hydrophilic protein in silkworm cocoons, which has the potential to be a new type of wound dressing. It has cytoprotective and mitogenic effects on fibroblasts and keratinocytes, and can promote fiber proliferation, making it beneficial to skin development. Attractive, in addition, sericin has good hydrophilicity, biocompatibility, biodegradability and good affinity for biomolecules, making the excellent moisture retention of sericin beneficial to accelerate wound healing.

The invention discloses an electronic cigarette liquid rich in biological active polysaccharide and a preparation method of the electronic cigarette liquid. The electronic cigarette liquid is mainly formed by the following raw materials in parts by weight: 50-70 parts of propylene glycol, 5-15 parts of glycerin, 5-10 parts of deionized water, 5-15 parts of natural biological active polysaccharide extracts, 3-10 parts of tobacco extracts, 1-5 parts of tobacco flavor, 1-3 parts of thickener and 1-3 parts of antioxidant. The electronic cigarette liquid disclosed by the invention is suitable for being smoked in an electronic atomizing way and is formed by refining the tobacco extracts and the natural biological active polysaccharide extracts, an immune function of a human body is also widely regulated while the feeling of smoking a cigarette is obtained by a consumer, and a certain healthcare function is obtained.

The invention discloses an extraction method of polysaccharide with activities of kidney warming and Yang tonifying from Aspongopus chinensis Dallas insects of Pentatomidae and application of the polysaccharide. The extraction method comprises the following steps: (1) drying Aspongopus chinensis Dallas insect bodies, performing pulverization, performing reflux extraction with petroleum ether, andconducting filtration liquid-solid separation so as to obtain drug residues with removal of grease; (2) evaporating the drug residues, performing reflux extraction with distilled water, performing filtration, combining the filtrate, and performing concentration so as to obtain total water extract; (3) adding distilled water to the total water extract for slight dilution, performing precipitation on polysaccharide with an alcohol solution, performing stirring while the alcohol solution is added, and performing still standing and filtration so as to obtain an insect polysaccharide alcohol precipitate; and (4) adding water to the insect polysaccharide alcohol precipitate for dilution and dissolution, performing chromatography separation and purification by using a DEAE-Sephadex ged cellulosecolumn, and performing rinsing with distilled water and a sodium chloride solution so as to obtain the Aspongopus chinensis Dallas polysaccharide. The invention also relates to the application of thepolysaccharide in preparation of drugs for treating or preventing Yang deficiency, impotence and erectile dysfunctions, the method is easy has simple and convenient operation, and the obtained polysaccharide compound with a purity of up to 95% has strong effects of kidney warming, Yang tonifying, fatigue resistance and body immunity recovery.

The invention discloses a collagen fiber capable of being directly used for spinning and dermal fiber leather prepared by the same. The collagen fiber is obtained by utilizing the strong effect of a polar solvent rich in hydroxyl groups and the combination of an ultrasonic treatment mode to achieve dissociation of intermolecular high-crystallized hydrogen bonds on the conditions of great mildness, obtaining raw material loosed in structure, performing drum treatment softening, liquid fiber separation and fiber drying, and then performing treatment on the dried collagen fiber in a single fiber state in combination with electrical impulse of an appropriate strength on the conditions of nitrogen protection. The preparation process is clean and free of pollution, and the manufactured collagen fiber can be directly spun through an existing spinning process without needing graft modification or compounding.

The invention belongs to the technical field of pigment extraction in natural raw materials and particularly relates to a method for extracting pigment by using a plant ash method. The method comprises the following steps: step one, treatment of pomegranate peel: washing, crushing and drying the pomegranate peel for later use; step two, preparation of a plant ash solution weighing plant ash of which the weight is 6 to 30 percent of pomegranate peel powder, and adding deionized water into a container containing the plant ash according to the solid-liquid ratio of the pomegranate peel powder tothe deionized water being (1 to 50) to 200(g / ml); step three, pigment extraction: adding the pomegranate peel powder into the plant ash solution for uniform stirring, putting the container in a thermostat water bath for extracting the pigment to obtain an extracting solution; concentrating and carrying out spray drying to obtain pomegranate peel pigment. By adopting the plant ash method to extract the pomegranate peel pigment, the mass percent of pigment extraction is 4.2 to 21.0 percent; the method has the advantages of environment-friendly and pollution-free extraction process, simple operation method and low cost of extracting pomegranate peel pigment.