High-performance starch/oxygen fuel cell based on microbial surface co-display sequential enzyme

A surface co-display, fuel cell technology, used in biochemical fuel cells, battery electrodes, circuits, etc., can solve problems such as limiting energy conversion efficiency, improve utilization, improve output power density and stability, and achieve efficient utilization Effect

Inactive Publication Date: 2017-02-22
QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the random immobilization of several single enzymes on the electrode greatly limits the energy conversion efficiency.

Method used

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  • High-performance starch/oxygen fuel cell based on microbial surface co-display sequential enzyme
  • High-performance starch/oxygen fuel cell based on microbial surface co-display sequential enzyme
  • High-performance starch/oxygen fuel cell based on microbial surface co-display sequential enzyme

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1) Preparation of bioanode

[0027] Bioanode Catalyst (GA&GDH-E.coli) is a sequential enzyme surface co-display system obtained by co-displaying the gene sequence (gdh-m) of glucoamylase (GA) and glucose dehydrogenase mutant on the surface of Escherichia coli (E.coli) ; Among them, the proportion of GA and GDH display numbers is 3:1.

[0028] The preparation of the above-mentioned sequential enzyme surface co-display system was obtained by referring to the method described in the publication No. 105624077A, entitled A sequential enzyme surface co-display system and its application.

[0029] First, 2 mg of MWNTs was added to 1 mL of DMF and sonicated until uniformly dispersed. The bare glassy carbon electrode (inner diameter 3mm) was polished to a mirror surface with 0.3 μm and 0.05 μm alumina powder in turn, rinsed with deionized water after polishing, and then ultrasonicated in anhydrous ethanol and ultrapure aqueous solution mixed at a volume ratio of 1:1 Wash, rins...

Embodiment 2

[0038] Construction and Testing of Starch / Oxygen Fuel Cells

[0039] The assembly of the starch / oxygen fuel cell was to simultaneously immerse the modified bioanode and biocathode in a solution containing 4mM NAD + , 0.5mM ABTS and 0.2M citric acid-Na 2 HPO 4 (pH=4.8) buffer solution. The measurement of its energy density and stability is done in the above solution saturated with oxygen.

[0040] In the detection process, the starch / oxygen fuel cell constructed above was based on GA&GDH-E.coli immobilized on polymethylene blue modified MWNTs as bioanode, and laccase immobilized on MWNTs as biocathode. Starch fuel is catalyzed by GA&GDH-E.coli on the bioanode, decomposed step by step, oxidized to gluconolactone, and releases electrons, while oxygen is reduced by laccase on the biocathode to generate positive charges. The battery supplies power to the external circuit to realize the energy conversion of starch fuel. The open circuit potential of this starch / oxygen fuel cell c...

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Abstract

The invention belongs to the field of bio-fuel cells, and particularly relates to a high-performance starch / oxygen fuel cell based on microbial surface co-display sequential enzyme. Escherichia coli (E. coli) surface co-display saccharifying enzyme and glucose dehydrogenase (GA&GDH-E. coli) are used as a biological anode catalyst, laccase serves as a biological cathode catalyst, and the biological anode catalyst and the biological cathode catalyst are respectively fixed onto a MWNTs (multi-walled carbon nanotubes) modified glassy carbon electrode to serve as a biological anode and a biological cathode. The obtained anode and the obtained cathode are assembled to form the starch / oxygen fuel cell. According to the obtained cell, chemical energy in starch is efficiently converted into electric energy, the energy in the starch is efficiently used and is clean and renewable energy, and an effective approach is provided for efficient use of other biomass fuels in nature.

Description

technical field [0001] The invention belongs to the field of biofuel cells, and specifically refers to a high-performance starch / oxygen fuel cell based on co-displaying sequential enzymes on the surface of microorganisms. Background technique [0002] Starch is a polysaccharide that widely exists in nature, and its content in plant seeds and tubers can be as high as 57%-75%. Realizing the conversion of starch to clean energy has high practical application value. In industry, fermentation technology is often used to convert starch into ethanol and gluconic acid to realize the conversion of starch into clean energy. However, the fermentation process usually needs to be carried out under conditions such as strong acid, strong alkali and high temperature, and is accompanied by various by-products, which greatly reduces the yield of ethanol and gluconic acid. [0003] As a device that directly converts chemical energy into electrical energy, biofuel cells can directly convert t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): H01M8/16H01M4/90
CPCH01M4/90H01M8/16Y02E60/50
Inventor 刘爱骅范树芹梁波侯传涛
Owner QINGDAO UNIV
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