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A kind of Pseudochlorella kaiseri tolerant to high pH and its cultivation application

A technology of Pseudochlorella and Kjeldahl, applied in the field of microbial engineering, can solve the problems of low efficiency and low solubility of carbon dioxide fixation, achieve good absorption and utilization of carbon dioxide, increase growth rate, and increase fixation effects

Active Publication Date: 2019-05-17
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The Pseudochlorella kelvinii selected by the present invention can better absorb and utilize carbon dioxide at a high pH value, grow and reproduce rapidly, and solve the problem that the existing Pseudochlorella kelvinia can only grow under a neutral pH, and the neutral pH Under PH conditions, there are problems of low solubility of carbon dioxide and low efficiency of fixing carbon dioxide

Method used

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  • A kind of Pseudochlorella kaiseri tolerant to high pH and its cultivation application
  • A kind of Pseudochlorella kaiseri tolerant to high pH and its cultivation application
  • A kind of Pseudochlorella kaiseri tolerant to high pH and its cultivation application

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Effect test

Embodiment 1

[0035] Example 1 Isolation and Screening of Pseudochlorella kescheri Mutant Strain FSH-Y3

[0036] The original Pseudochlorella kelvinii algae strain of the present invention is the wild Pseudochlorella kelvinia kelvinia isolated from water samples collected from the Hunhe River in Fushun City, Liaoning Province in November 2011 by means of plate marking, and the number is FSH-3 . The original Pseudochlorella kesperi strain FSH-3 was subjected to ultraviolet mutagenesis and acclimatized culture to select the mutant strain FSH-Y3 of Pseudochlorella kesperi with tolerance to high pH.

[0037] The method of obtaining the Pseudochlorella kescheri mutant strain FSH-Y3 of the present invention is specifically as follows:

[0038] (1) Prepare BG11 medium, divide the prepared BG11 medium into 250mL shake flasks, each bottle has a liquid volume of 100mL, seal it with a gas-permeable film, wrap it in kraft paper, and sterilize it at 121°C and 0.1MPa for 30 minute. The composition of ...

Embodiment 2

[0048] Embodiment 2 Comparison of the growth rate of the original algae strain and the mutagenized algae strain at different pH

[0049] The algae liquid in the logarithmic growth phase of the screened algae strain FSH-Y3 and the algae liquid in the logarithmic growth phase of the original algae species FSH-3 were inserted into the shake flask containing the BG11 medium according to a certain ratio. After inoculation, the initial OD 680 The value is the same, both are 0.2. CO was introduced from the bottom of the reactor 2 The gas content is 5v%, cultured for 7 days under different pH values, and the OD is measured 680 The value is used as an index to measure the growth rate, and the results are shown in Table 3.

[0050] Table 3 Comparison of OD values ​​between original algal strains and mutagenic algal strains at different pH values

[0051]

[0052] It can be seen from Table 3 that the OD value of the mutagenized algal strains did not change significantly with the i...

Embodiment 3

[0053] The comparison of the growth effect of embodiment 3 FSH-Y3 and FSH-3

[0054] The algae fluids of FSH-Y3 and FSH-3 in the logarithmic growth phase were inoculated on BG11 medium for cultivation, and the cultivation was carried out in a laboratory-made column reactor (with an inner diameter of 40 mm and a height of 500 mm). Density adjusted to OD 680 is 0.2. According to the needs of the experiment, mix different contents of CO 2 The gas is then introduced from the bottom of the reactor. During the cultivation process, the light intensity was 5000Lux, the culture temperature was 28°C, the pH value was controlled at 11, the light cycle was 24h, the light-dark time ratio was 14:10, and the culture time was 7 days. After vacuum freeze-drying at ℃ to constant weight, the dry weight of algae powder was measured to calculate the biomass production. The results are shown in Table 4.

[0055] Table 4 Different CO 2 content effect comparison

[0056]

[0057] It can be ...

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Abstract

The invention discloses parachlorella kessleri with the tolerance to a high pH and culture and applications of parachlorella kessleri. A parachlorella kessleri mutant strain FSH-Y3 with the tolerance to the high pH is obtained through the method adopting ultraviolet mutagenesis and acclimation screening utilizing step-by-step pH increase, the classification name is Parachlorella Kessleri, and the parachlorella kessleri strain FSH-Y3 is prepared in the China General Microbiological Culture Collection Center (CGMCC) on May 26, 2014, and is assigned with the accession number of CGMCC No.9238. The bred parachlorella kessleri FSH-Y3 can well absorb and utilize carbon dioxide at the high pH, and rapidly grow and reproduce, therefore, the problems that the existing parachlorella kessleri only can grow at the neutral pH, but the solubility of carbon dioxide is low, and the carbon dioxide fixing efficiency is low under the neutral pH condition are solved.

Description

technical field [0001] The invention belongs to the field of microbial engineering, and in particular relates to a high pH-tolerant Pseudochlorella kelvinii and its cultivation application. Background technique [0002] With the development of the world economy, the use and consumption of a large amount of fossil energy leads to the shortage of energy and the deterioration of the environment, especially CO 2 The greenhouse effect caused by the sharp increase of microalgae is becoming more and more serious, the growth cycle of microalgae is short, the photosynthetic efficiency is high, and the CO 2 High fixation efficiency, up to 10 times that of terrestrial plants under certain conditions, not only can reduce CO 2 Emissions, but also reduce the cost of cultivation; in addition to CO 2 In addition, some SOx, NOx and other components in the exhaust gas are also purified with the metabolism of microalgae, effectively reducing harmful gas emissions. Therefore, biodiesel produc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C12N15/01C12N13/00C12P7/64C12R1/89
Inventor 师文静廖莎孙启梅王鹏翔李晓姝樊亚超张霖
Owner CHINA PETROLEUM & CHEM CORP
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