B-cell epitope of tnf-α protein, multi-antigen peptide containing this epitope and application

A multi-antigen and B cell technology, applied in the field of immunology and gastroenterology, can solve the problems of difficult humoral immune response, short epitope peptide, small molecular weight, etc., and achieve the effect of low price, convenient administration and less frequency

Active Publication Date: 2019-08-09
ZHEJIANG PROVINCIAL PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with macromolecular antigens, self-antigens have defects such as natural immune tolerance, smaller molecular weight, shorter epitope peptides that are more difficult to screen, and less likely to cause humoral immune responses. Therefore, researchers in this field have never applied the MAP design scheme. self small molecule antigen

Method used

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  • B-cell epitope of tnf-α protein, multi-antigen peptide containing this epitope and application
  • B-cell epitope of tnf-α protein, multi-antigen peptide containing this epitope and application
  • B-cell epitope of tnf-α protein, multi-antigen peptide containing this epitope and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: Screening of dominant epitopes of human TNF-α B cell epitopes and construction of multiple antigenic peptides.

[0024] ①Search the NCBI GenBank database to obtain the amino acid sequence of human TNF-α protein; use DNAStar, GOLDENKEY (Academy of Military Medical Sciences) software and the computer vaccine design tool Bcepred on the Internet to analyze and obtain the most antigenic amino acid sequence information in the hydrophilic region and B cell epitope; according to the prediction of antigenic epitope, select a peptide rich in hydrophilic amino acids with a length of about 6-25 amino acids, while avoiding more than 4 consecutive adjacent hydrophobic amino acid residues and containing more than 2 and a half Peptides of cystine, so as not to form complex multimers through disulfide bonds.

[0025] ② Analysis software ( figure 1 ) and web design tools ( figure 2 ) prediction results. Taking 13 amino acid residues as a group, analyze its hydrophilicity, ...

Embodiment 2

[0032] Example 2: Verification of the in vivo and in vitro immunological effects of the multi-antigen peptide constructed by the method described in Example 1.

[0033] Due to the high homology between rats and human TNF-α, this embodiment takes rats as the research object, screens and identifies the dominant epitope according to the method described in Example 1, and then uses its dominant epitope to synthesize The TNF-α B cell epitope MAP multi-antigen peptide vaccine was used to verify the immunological effect of rats in vivo and in vitro, so as to prove the positive effect of the multi-antigen peptide with 8-branched peptide conformation constructed by the present invention in human and mouse ulcerative colitis.

[0034] Among them, the in vitro effect research includes: the effect of MAP antibody on the killing of mouse fibroblast L929 by TNF-α in vitro; the effect of MAP antibody on the dissolution of 3H-TdR-labeled liver cancer cell HCC97H by TNF-α in vitro. The in vivo...

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Abstract

The invention provides TNF-Alpha protein B cell epitopes, an 8-branch-peptide-conformation multiple antigen peptide synthesized on the basis of the TNF-Alpha protein B cell epitopes combining with an MAP (multiple antigen peptide) scheme, and application of the multiple antigen peptide in the preparation of ulcerative colitis vaccines. The MAP scheme is creatively applied to the field of immunological research on small molecular autoantigens, and human TNF-Alpha protein B cell dominant epitopes are screened out through a large amount of tests. By using the MAP scheme on such basis, 8-branch peptide conformation with lysine as the core matrix is acquired, the 8-branch-peptide-conformation multiple antigen peptide belongs to autoantigen epitope vaccines, and accordingly the 8-branch-peptide-conformation multiple antigen peptide is convenient in administration, few in administration times and capable of inducing antibody generation by small-quantity injection; the multiple antigen peptide is also good in safety and tolerance, capable of achieving both preventing and treating, and the like; an important theoretical basis is hopefully provided for the research and development of autoantigen MAP vaccines, and a new through can be provided for the clinical treatment of ulcerative colitis.

Description

technical field [0001] The invention belongs to the field of immunology and gastroenterology, and specifically relates to a B cell epitope of TNF-α protein, and a multi-antigen peptide in an 8-branched peptide conformation synthesized based on the epitope in combination with the MAP scheme, and in the preparation of ulcerative Use in colitis vaccines. Background technique [0002] TNF-α is a cytokine with complex biological activities. In addition to killing tumor cells in vivo and in vitro, it also has multiple functions such as inducing inflammatory responses, antiviral infection, regulating body immunity, and promoting cell proliferation and activation. However, studies have shown that when TNF-α is overexpressed, it will synergistically produce a variety of pathological injuries, such as inflammatory bowel disease, infectious disease, dyscrasia, etc.; at the same time, TNF-α is also a therapeutic target The human body's own antigens can neutralize the excessive TNF-α in...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/525A61K39/00A61P1/00
CPCA61K39/0005C07K14/525
Inventor 张骏张运龙崔盈陶厚权王惠菊
Owner ZHEJIANG PROVINCIAL PEOPLES HOSPITAL
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