Electrochemical DNA biosensor for detecting arthrobotrys oligospora and application thereof

A biosensor and electrochemical technology, applied in the field of DNA biosensors, can solve the problems of poor crop yield improvement, restriction of agricultural and forestry development, environmental pollution, etc., and achieve good application and development prospects, good selectivity, and easy operation Effect

Inactive Publication Date: 2017-03-15
YUNNAN UNIV
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Problems solved by technology

In my country, root-knot nematode, pine wood nematode and cyst nematode are the main plant parasitic nematode diseases, which lead to a large reduction in crop yield and limit the development of my country's agricult

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  • Electrochemical DNA biosensor for detecting arthrobotrys oligospora and application thereof

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Embodiment Construction

[0047] The present invention will be described in further detail below in conjunction with the accompanying drawings.

[0048] One, the electrochemical DNA biosensor of the present invention comprises successively from inside to outside: glassy carbon electrode, manganese dioxide nanoparticle dispersion liquid layer, probe DNA solution layer; Obtain through the following steps:

[0049] 1. Preparation of nano-manganese dioxide

[0050] Add manganese acetate tetrahydrate and potassium permanganate in a molar ratio of 3:2 to an agate mortar and grind for 40 minutes. After the reaction was complete, the powder was washed in sequence with absolute ethanol and ultrapure water in a water bath at 80° C. for 6 hours. Cycle 3 times. Finally, dry in a drying oven to obtain nano manganese dioxide particles.

[0051] 2. Manganese dioxide|chitosan|glassy carbon modified electrode preparation

[0052] Prepare 0.05mol / L acetic acid solution. According to dissolving 0.5g chitosan in ever...

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Abstract

The invention discloses an electrochemical DNA biosensor for detecting arthrobotrys oligospora and an application thereof. The electrochemical biosensor consists of a glassy carbon electrode, a manganese dioxide nanoparticle dispersion liquid layer and a probe DNA solution layer and is prepared by the following steps: preparation of nano manganese dioxide, preparation of a manganese dioxide/chitosan/glassy carbon electrode, and preparation of a modified electrode single-stranded DNA/manganese dioxide/chitosan/glassy carbon electrode; and after the electrochemical biosensor is hybridized with target DNA, the surface electron transfer resistance change before and after modification of the glassy carbon electrode is detected by use of an electrochemical impedance spectrum as a detection signal for detecting the target DNA. The electrochemical DNA biosensor is used for identifying the strain of nematode-trapping fungus arthrobotrys oligospora (A.oligospora). The invention has the following advantages: the electrochemical DNA biosensor is simple and easy to operate and responds quickly, the electrode has good selectivity, the probe DNA has high specificity, and the electrochemical DNA biosensor can be used for identifying the strain of nematode-trapping fungus arthrobotrys oligospora (A.oligospora). The invention has a good application development prospect.

Description

technical field [0001] The invention relates to an electrochemical DNA biosensor and its application, belonging to the field of DNA biosensors. Background technique [0002] Plant parasitic nematodes cause plant diseases and are one of the main sources of plant diseases. Plant-parasitic nematodes cause crop yield losses in excess of $157 million annually in the United States (1). In my country, root-knot nematodes, pine wood nematodes and cyst nematodes are the main plant parasitic nematode diseases, which lead to a large reduction in crop yield and limit the development of agriculture and forestry in my country (2). In the past, the control of nematodes mainly relied on chemical insecticides, but long-term use of chemical insecticides caused environmental pollution, and crop yields have not been improved very well. At the same time, toxin residues affect the health of animals, plants, and even humans. In the past ten years, the governments of various countries have paid ...

Claims

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Application Information

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IPC IPC(8): G01N27/26G01N27/327
CPCG01N27/26G01N27/3275
Inventor 屈庆陈莹李蕾张克勤
Owner YUNNAN UNIV
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