The invention discloses a molybdenum disulfide diaphragm of a lithium-sulfur battery and a preparation method therefor, belonging to the field of lithium-sulfur batteries. According to the molybdenum disulfide diaphragm of the lithium-sulfur battery, the molybdenum disulfide diaphragm has high lithium conductivity, and is capable of inhibiting the migration of polysulfides between a positive electrode and a negative electrode so as to improve a cycle life of the lithium-sulfur battery. In addition, the high-temperature resistance of molybdenum disulfide is used to improve the overall temperature resistance of the diaphragm; a vacuum filtration method is used to deposit a molybdenum disulfide nanosheet on the surface layer of the lithium-sulfur battery diaphragm; and a relative position of pore size of a funnel on the surface of the molybdenum disulfide diaphragm is relatively small in thickness under the vacuum filtration condition, so that good air permeability can be achieved, and battery capacity is not affected. The MoS2/Celgard prepared is applied to the lithium-sulfur battery; an electrochemical impedance spectroscopy result shows that the lithium ion conductivity of the MoS2/Celgard diaphragm is about 2.0*10<-1> mS.cm<-1>, thereby substantially prolonging the cycle life of the lithium-sulfur battery. The whole preparation method is short in process flows, the conditions are simple, the cost is low, and no pollution is caused to the environment, so that a requirement of cleaner production can be achieved.