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Method for detecting residuals of sulfanilamide medicaments by portable imaging method and applications thereof

A technology of sulfonamide drugs and imaging method, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of rapid determination of animal food drug residues that have not yet been seen, unsuitable for rapid inspection, cumbersome sample pretreatment, etc., to achieve outstanding essence characteristics, low testing costs, and the effect of ensuring food safety

Inactive Publication Date: 2017-03-29
YANTAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods have high accuracy and precision, but the required sample pretreatment is cumbersome, lengthy, and requires expensive instruments and equipment, which is not suitable for rapid on-site inspection
Thin-layer chromatography has the characteristics of high separation efficiency and simple operation. Thin-layer chromatography imaging technology has been widely used in the separation and identification of biological substances, but it has not been used for the rapid determination of animal food drug residues.

Method used

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  • Method for detecting residuals of sulfanilamide medicaments by portable imaging method and applications thereof
  • Method for detecting residuals of sulfanilamide medicaments by portable imaging method and applications thereof
  • Method for detecting residuals of sulfanilamide medicaments by portable imaging method and applications thereof

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Embodiment 1

[0042] This embodiment measures the linear range and detection limit of sulfa drugs, and calculates the working curve equation through this embodiment at the same time, and establishes the working curve.

[0043] The working curve equation was established as follows,

[0044] Preparation of sulfonamide standard solution: select sulfonamide drug standard substance, then dissolve and shake with acetonitrile and constant volume to prepare sulfonamide standard solution, then use a pipette to accurately absorb different amounts of sulfonamide standard solution to prepare gradient concentration of sulfonamide standard solution;

[0045]Preparation of the test solution: wild carp was used as a blank sample, and the fish meat was homogenized, and five parts of 1.0g fish meat samples were accurately weighed in 10mL polytetrafluoroethylene tubes, and different concentrations of sulfonamide standard solutions were added to prepare For samples with scalar amounts of 0, 0.1, 0.4, 0.8, and ...

Embodiment 2

[0062] Embodiment 2 The detection test of the present invention to the recovery rate of standard addition of fish meat samples

[0063] Before the test, first select the standard sulfonamide drug, then dissolve it with acetonitrile, shake it up and prepare the sulfonamide standard solution at constant volume.

[0064] Preparation of the test solution: another wild carp was taken as a blank sample, and the fish meat was homogenized, and three parts of 1.0g fish meat samples were accurately weighed in 10mL polytetrafluoroethylene tubes, and sulfonamide standard solutions of different concentrations were added respectively. Prepare samples with the addition of 0.25, 0.5, and 0.8 μg / g, mix well and let stand for 30 minutes, then add 70% acetonitrile aqueous solution, the specific ratio of 70% acetonitrile aqueous solution is to add 2.5mL acetonitrile to 1mL water , and then add 1.0g of anhydrous ammonium sulfate respectively, oscillate and vortex for 10min, then press 5000r·min -...

Embodiment 3

[0071] Embodiment 3 The present invention is to the detection test of pork sample spiked recovery rate

[0072] Before the same test, first select the sulfonamide drug standard substance, then dissolve it with acetonitrile and shake it up and prepare the sulfonamide standard solution at constant volume.

[0073] Preparation of the test solution: select non-polluted pork as a blank sample, then homogenize the pork, accurately weigh 3 parts of 1.0g pork samples in 10mL polytetrafluoroethylene tubes, add different concentrations of sulfonamide standard Solution, prepared into samples with a scalar amount of 0.2, 0.5, 0.8 μg / g respectively, mix well and let stand for 30 minutes, then add 70% acetonitrile aqueous solution respectively, the specific ratio of 70% acetonitrile aqueous solution is 1mL water to add 2.5mL Acetonitrile, and then add 1.0g of anhydrous ammonium sulfate, shake, vortex and mix for 10min, then press 5000r·min -1 After centrifugation for 10min, take 1.5mL of e...

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Abstract

The invention provides a novel method for rapidly detecting and calculating content of sulfanilamide by detecting luminous intensity of sulfanilamide medicament imaging and establishing working curves, in order to solve the problems existed in the prior art for sulfanilamide medicament detection. The method can be used for accurately and economically detecting contents of sulfanilamide medicaments in animal food, especially is suitable for detecting contents of four sulfanilamide medicaments including sulfathiazole, sulfapyridine, sulphadiazine and sulfamerazine. The invention provides applications of the accurate, economic, high efficient, rapid and portable determination of residuals of the four sulfanilamide medicaments in animal food.

Description

technical field [0001] The invention relates to the technical field of animal food drug residue analysis, in particular to a rapid determination method for sulfonamide drug residues. Background technique [0002] Sulfonamides are a class of synthetic drugs with broad-spectrum antibacterial activity, which are widely used in the prevention and treatment of infectious diseases in animals. Sulfonamide veterinary drugs are easy to take, can be taken orally or injected, absorb quickly, have high curative effect, broad antibacterial spectrum, and low price. Sulfonamide veterinary drugs have a long residual half-life in food and have strong toxic and side effects. [0003] The current main methods for the determination of sulfa drug residues are high performance liquid chromatography, liquid-mass spectrometry, etc. These methods have high accuracy and precision, but the required sample pretreatment is cumbersome, lengthy, and requires expensive instruments and equipment, which is...

Claims

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Application Information

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IPC IPC(8): G01N30/90
CPCG01N30/90
Inventor 殷军港刘永明赵烽王湘敏赵峰樊振华宋跃进
Owner YANTAI UNIV
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