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A column packing method for reverse phase chromatography column

A reversed-phase chromatography and column packing technology, which is applied in separation methods, chemical instruments and methods, measuring devices, etc., can solve the problems that the symmetry is difficult to reach the standard of 0.8 to 1.8, cannot meet the requirements, and is difficult to pack evenly. The effect of column packing is good, the repeated packing is reduced, and the packing operation is simple.

Active Publication Date: 2019-01-18
SHENZHEN WATSIN GENETECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For reversed-phase chromatography packing, due to its high pressure resistance (0-10MPa), it is difficult to fill evenly during the installation process, and the symmetry is difficult to reach the standard of 0.8-1.8. In many cases, repeated filling may still fail to meet the requirements

Method used

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  • A column packing method for reverse phase chromatography column
  • A column packing method for reverse phase chromatography column
  • A column packing method for reverse phase chromatography column

Examples

Experimental program
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Effect test

Embodiment 1

[0028] Such as figure 1 As shown, this embodiment provides a column packing method for reverse phase chromatography column 1, comprising the following steps:

[0029] 1) The slurry of the reversed-phase filler is loaded into the reversed-phase chromatography column 1;

[0030] 2) Carrying out bubble removal on the column head 2 of the reverse-phase chromatography column;

[0031] 3) Compact the reversed-phase filler in the reversed-phase chromatography column 1 .

[0032] The column packing method of the reverse phase chromatography column 1 in this embodiment is to remove the air bubbles from the column head 2 of the reverse phase chromatography column, so that the column packing operation of the reverse phase chromatography column 1 is simple, the column packing effect is good, and repetition can be reduced. Column packing saves operating time, manpower and material resources.

[0033] Specifically, the reverse-phase chromatography column 1 in this example is a FINELINE c...

Embodiment 2

[0049] Such as figure 1 As shown, this embodiment provides a column packing method for reverse phase chromatography column 1, comprising the following steps:

[0050] 1) The slurry of the reversed-phase filler is loaded into the reversed-phase chromatography column 1;

[0051] 2) Carrying out bubble removal on the column head 2 of the reverse-phase chromatography column;

[0052] 3) Compact the reversed-phase filler in the reversed-phase chromatography column 1 .

[0053] The column packing method of the reverse phase chromatography column 1 in this embodiment is to remove the air bubbles from the column head 2 of the reverse phase chromatography column, so that the column packing operation of the reverse phase chromatography column 1 is simple, the column packing effect is good, and repetition can be reduced. Column packing saves operating time, manpower and material resources.

[0054] Specifically, the reverse-phase chromatography column 1 in this example is a FINELINE c...

Embodiment 3

[0069] Such as figure 1 As shown, this embodiment provides a column packing method for reverse phase chromatography column 1, comprising the following steps:

[0070] 1) The slurry of the reversed-phase filler is loaded into the reversed-phase chromatography column 1;

[0071] 2) Carrying out bubble removal on the column head 2 of the reverse-phase chromatography column;

[0072] 3) Compact the reversed-phase filler in the reversed-phase chromatography column 1 .

[0073] The column packing method of the reverse phase chromatography column 1 in this embodiment is to remove the air bubbles from the column head 2 of the reverse phase chromatography column, so that the column packing operation of the reverse phase chromatography column 1 is simple, the column packing effect is good, and repetition can be reduced. Column packing saves operating time, manpower and material resources.

[0074] Specifically, the reverse-phase chromatography column 1 in this example is a FINELINE c...

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Abstract

The invention discloses a column mounting method of a reverse phase chromatographic column. The column mounting method comprises the following steps that (1) serous fluid of reverse phase filler is poured into the reverse phase chromatographic column; (2) bubbles are removed out of the column head of the reverse phase chromatographic column; and (3) the reverse phase filler in the reverse phase chromatographic column is compacted. According to the column mounting method of the reverse phase chromatographic column, the bubbles are removed out of the column head of the reverse phase chromatographic column, so that the column mounting operation of the reverse phase chromatographic column is easy, the column mounting effect is good, repeated column mounting can be omitted, operation time is shortened, and manpower and material resources are reduced.

Description

technical field [0001] The invention belongs to the technical field of separation, purification and analysis, and in particular relates to a method for packing a reverse phase chromatography column. Background technique [0002] Reverse chromatography is widely used in the separation, purification and analysis of proteins, peptides and oligonucleotides, but the packing effect of the reverse phase chromatography column directly affects the yield and quality of the product. The quality of reverse phase chromatography column packing is mainly measured by two indicators: theoretical plate number and symmetry. Usually, for a good column efficiency, the number of theoretical plates > 1 / 3×1 / average particle size of the packing, and the symmetry is 0.8-1.8, so the choice of column packing method is particularly important. For reversed-phase chromatography packing, due to its high pressure resistance (0-10MPa), it is difficult to fill evenly during the installation process, and t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01D15/10G01N30/89
CPCB01D15/10G01N30/89
Inventor 史小敏闻亚磊王树毅叶学君李盖
Owner SHENZHEN WATSIN GENETECH