Application of elvitegravir to preparation of drugs for preventing or treating ALI/ARDS (acute lung injury/acute respiratory distress syndrome) and PF (pulmonary fibrosis)
A technology for acute respiratory distress and acute lung injury, which is applied in the application field of elvitegravir in the preparation of drugs for the prevention or treatment of acute lung injury/acute respiratory distress syndrome and pulmonary fibrosis. And other issues
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[0010] Experimental Example 1 The effect of eptigovir on acute lung injury / acute respiratory distress syndrome (ALI / ARDS) model rats caused by cecal ligation and perforation
[0011] 1.1 Drugs and reagents
[0012] Etegavir (purity 99.5%, purchased from Shanghai Hanxiang Biotechnology Co., Ltd.)
[0013] Limulus Reagent Kit (Fuzhou Xinbei Biochemical Industry Co., Ltd., Fujian Province, batch number: 080430)
[0014] Anti-Aquaporin 5 antibody (abcam, ab104751)
[0015] Experimental animals: SPF-grade Sprague Dawley rats, male, weighing 150g-200g, provided by the Experimental Animal Center of Shandong Luye Pharmaceutical Co., Ltd., the animal certificate number is: SYXK (鲁)20030020.
[0016] 1.2 Experimental methods and results
[0017] 1.2.1 Preparation of cecal ligation and perforation (CLP) rats
[0018] In the early morning of the operation, the rats were fasted and placed supine after anesthesia with ether. The abdomen was disinfected with 75% ethanol. A 1.5cm incision was made along t...
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