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Pavlova viridis medium composition

A green Pavlova, culture medium technology, applied in microorganism-based methods, microorganisms, unicellular algae, etc., can solve the problems of low yield and slow algal cell growth, achieve high cell density, promote rapid proliferation, algae The effect of increased cell concentration

Inactive Publication Date: 2017-05-10
NINGBO FUTIAN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The inventor of the present application found that the algae cell growth rate of Pavlova viridis was slow and the yield was low when using MAV culture solution to cultivate Pavlova viridis

Method used

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Experimental program
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Effect test

Embodiment approach 1

[0014] A green Pavlova culture medium composition, comprising the following components: KNO 3 : 300mg / L; KH 2 PO 4 : 10mg / L; FeSO 4 ·7H 2 O: 2.5mg / L; MnSO 4 : 0.25g / L; MgSO 4 : 0.075g / L; EDTA-Na 2 : 10mg / L; VB 1 :6mg / L; VB 12 : 50ug / L; 6-BA that promotes plant growth regulation: 0.1mg / L; glycine as an additive: 0.5mg / L and proline as an osmotic regulator: 0.1mg / L.

[0015] Configure the green Pavlova culture medium composition prepared by the above-mentioned scheme and 1000ml of filtered seawater. Then take Pavlova viridis in the logarithmic growth phase and inoculate it into the medium for cultivation. The light intensity is about 50 μmol / (m2 s) for 12 hours a day, and the temperature of the incubator is controlled at 25°C. Cell concentration of Pavlova viridis in culture medium.

Embodiment approach 2

[0017] A green Pavlova culture medium composition, comprising the following components: KNO 3 : 400mg / L; KH 2 PO 4 : 10mg / L; FeSO 4 ·7H 2 O: 2.5mg / L; MnSO 4 : 0.25g / L; MgSO 4 : 0.075g / L; EDTA-Na 2 : 10mg / L; VB 1 :6mg / L; VB 12 : 50ug / L; Promoting plant growth regulator 6-BA: 1mg / L; Glycine as additive: 3mg / L and proline as osmotic adjustment: 1mg / L.

[0018] Configure the green Pavlova culture medium composition prepared by the above-mentioned scheme and 1000ml of filtered seawater. Then take Pavlova viridis in the logarithmic growth phase and inoculate it into the medium for cultivation. The light intensity is about 50 μmol / (m2 s) for 12 hours a day, and the temperature of the incubator is controlled at 25°C. Cell concentration of Pavlova viridis in culture medium.

Embodiment approach 3

[0020] A green Pavlova culture medium composition, comprising the following components: KNO 3 : 300mg / L; KH 2 PO 4 : 10mg / L; FeSO 4 ·7H 2 O: 2.5mg / L; MnSO 4 : 0.25g / L; MgSO 4 : 0.075g / L; EDTA-Na 2 : 10mg / L; VB 1 :6mg / L; VB 12 : 50ug / L; plant growth regulator 6-BA: 0.5mg / L; additive glycine: 1.75mg / L and osmotic regulator proline: 0.5mg / L.

[0021] Configure the green Pavlova culture medium composition prepared by the above-mentioned scheme and 1000ml of filtered seawater. Then take Pavlova viridis in the logarithmic growth phase and inoculate it into the medium for cultivation. The light intensity is about 50 μmol / (m2 s) for 12 hours a day, and the temperature of the incubator is controlled at 25°C. Cell concentration of Pavlova viridis in culture medium.

[0022] In order to better reflect the advantage that this technical scheme has fast growth rate and high yield compared with the traditional MAV medium, the seawater MAV medium is used as the control medium, and t...

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Abstract

The invention discloses a pavlova viridis medium composition, which consists of the following component: 300-40mg / L of KNO3, 10mg / L of KH2PO4, 2.5mg / L of FeSO4.7H2O, 0.25g / L of MnSO4, 0.075g / L of MgSO4, 10mg / L of EDTA Na2, 6mg / L of VB1, 50 [mu]g / L of VB12, 0.1-1mg / L of a plant growth regulator, 0.5-3mg / L of an additive and 0.1-1mg / L of an osmotic regulating agent. According to the technical scheme, the prepared pavlova viridis medium composition, when used for breeding pavlova viridis, has the characteristics of high growth rate, high cell density and high chlorophyll content of pavlova viridis.

Description

technical field [0001] The invention belongs to the field of marine microalgae cultivation, in particular to a green Pavlova culture medium composition. Background technique [0002] Green Pavlova viridid ​​belongs to Chrysophyta, Prymnesiophyceae, Pavlovales, Pavolovaceae. The algae is a marine algae without cell wall, small individual (6.0×4.8×4.0μm), and reproduces quickly. Pavlova viridis has high protein and high fatty acid content, which is very suitable for the feeding and digestion of aquatic animal larvae, especially rich in docosahexaenoic acid EPA, which is necessary for the growth and development of aquatic economic animal larvae, and is a high-quality unit cell Algae bait is widely used in artificial breeding of shrimp, crab, shellfish larvae and seawater larvae. Because green Pavlova is rich in active ingredients such as special Pavsterol and polysaccharides, it also has important research value in terms of biological activity. [0003] The inventors of the ...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 王兆伟宋佳巍王弢
Owner NINGBO FUTIAN BIOTECH
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