Advenella kashmirensis, microbial agent and application of advenella kashmirensis and microbial agent

A technology of marine oil and degrading bacteria, applied in the field of bioengineering, can solve the problems of microbial reproduction and metabolic activity being greatly affected, death, etc., and achieve good application prospects, strong environmental adaptability, and good degradation and repair effects

Active Publication Date: 2017-05-10
陕西澳美环保科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, there are still some limitations in the rapid development of microbial remediation technology. Specific microorganisms can only degrade specific types of compounds. mass death

Method used

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  • Advenella kashmirensis, microbial agent and application of advenella kashmirensis and microbial agent
  • Advenella kashmirensis, microbial agent and application of advenella kashmirensis and microbial agent
  • Advenella kashmirensis, microbial agent and application of advenella kashmirensis and microbial agent

Examples

Experimental program
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Effect test

Embodiment 1

[0022] Example 1 Separation and Purification of Bacterial Strains

[0023] (1) Source of strain

[0024] The present invention provides a marine oil-degrading bacterium (Advenella kashmirensis) 6-10-1, which is isolated from crude oil polluted soil at the Qilidong oil production field oil collection station of Yanchang Crude Oil Group. The physical and chemical properties of the soil are: ammonium nitrogen 15.09ppm, Nitrate nitrogen 0.72ppm; nitrogen content 15.81ppm, phosphorus content 82.05ppm, potassium content 17.45ppm, organic matter content 1.43%, pH 7.65.

[0025] (2) Isolation and purification of bacterial strains

[0026] Add 10 g of crude oil contaminated soil samples into 100 mL of enriched liquid medium, and incubate on a shaker at 28 °C and 130 r / min for 7 days. After the culture medium became turbid, pipette 5 mL of the culture medium and re-transfer it into a fresh enrichment medium, and carry out continuous transfer enrichment culture for 3 times under the sa...

Embodiment 2

[0029] Identification of embodiment 2 strains

[0030] (1) Morphological characteristics of the strain

[0031] Such as figure 1 As shown, the marine oil-degrading bacteria were cultured on beef extract peptone medium at a constant temperature of 30°C for 24 hours to observe the colony morphology, and the bacterial strain 6-10-1 was round, with complete edges, smooth and opaque on the plate of beef extract peptone , milky white, moist surface, no special smell. Such as figure 2 As shown, the bacteria are short rod-shaped, with a size of (0.41-1.02) μm×(0.41-1.53) μm, without flagella.

[0032] (2) Biochemical properties

[0033] According to the "Bergey's Bacteria Identification Manual" and "Common Bacteria System Identification Manual", the physiological and biochemical identification of marine oil-degrading bacteria was carried out, and the identification results are shown in Table 1.

[0034] Table 1 Physiological and biochemical identification results of marine oil-d...

Embodiment 3

[0041] Example 3 Degradation of No. 1 Crude Oil Polluted Water by Advenella kashmirensis 6-10-1

[0042] experiment method:

[0043] (1) Preparation of Advenella kashmirensis 6-10-1 bacterial suspension

[0044] Under sterile conditions, the marine oil-degrading bacteria obtained by separation and purification were picked, inoculated in 100 mL of beef extract peptone liquid medium, and expanded on a shaking table for 48 hours; the composition of the beef extract peptone liquid medium was: beef extract 3.0 g, peptone 10.0g, NaCl 5.0g, nutrient agar 15.0g, distilled water 1000mL, pH 7.4-7.6. The pH value is adjusted to the required value with 1mol / L sodium hydroxide or 1mol / L hydrogen chloride. Wherein, the medium sterilization conditions are: the temperature is 121-126° C., and the time is 25 minutes.

[0045] (2) Determination of No. I Crude Oil Maximum Absorption Wavelength

[0046] Petroleum and its products have certain absorption characteristics in the ultraviolet regi...

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Abstract

The invention belongs to the technical field of bioengineering, and particularly discloses advenella kashmirensis 6-10-1. The advenella kashmirensis is preserved in China General Microbiological Culture Collection Center of Institute of Microbiology of Chinese Academy of Sciences, and has a preservation number of CGMCC No.13003. The invention further discloses application of the advenella kashmirensis and a microbial agent thereof to degradation on crude oil pollution water. The advenella kashmirensis disclosed by the invention is high in degradation capacity on the crude oil pollution water, and is high in environmental adaptability; meanwhile, in the process that the advenella kashmirensis degrades the crude oil pollution water, degradation concentration on crude oil in the pollution water reaches 1mL / 100mL, and recovery processing of the crude oil pollution water can be effectively implemented; the solid microbial agent of the advenella kashmirensis also has an excellent degradation repair effect on crude oil pollution soil, implements repair processing of crude oil pollution, and shows wide application prospect in biological repair of the crude oil pollution.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a marine oil-degrading bacterium, a bacterium agent and applications thereof. Background technique [0002] The alkanes, aromatic hydrocarbons, sulfur-containing compounds and heterocyclic compounds containing sulfur or nitrogen contained in petroleum are all toxic and extremely harmful to humans and the environment. Crude oil is unprocessed petroleum, which is a viscous black or dark brown liquid or solid formed by natural organic substances through geological changes. Generally, most of them exist underground or on the seabed, and have a pungent smell. The main component is a chain compound formed by the combination of carbon and hydrogen atoms, which is extremely harmful to water bodies. [0003] At present, the remediation technologies for oil-polluted water bodies mainly include physical remediation technology, chemical remediation technology, photocatalytic remediat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01
CPCC12N1/20C02F3/34C12R2001/01C12N1/205
Inventor 李春荣张帆王文科申圆圆邓红章张慧慧姬雨王广华郭静茹
Owner 陕西澳美环保科技有限公司
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