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A molecular marker and method for identifying wheat ear germination resistance

A technology of ear germination and wheat, which is applied in the field of wheat molecular breeding, can solve the problems of quality deterioration, wheat yield decline, grower economic loss, etc., and achieve the effect of enriching the genetic resource bank

Active Publication Date: 2020-06-09
ANHUI AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The yield of wheat with ear germination decreases and the quality deteriorates, causing serious economic losses to growers

Method used

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  • A molecular marker and method for identifying wheat ear germination resistance
  • A molecular marker and method for identifying wheat ear germination resistance
  • A molecular marker and method for identifying wheat ear germination resistance

Examples

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Embodiment 1

[0021] 1. Materials

[0022] The methods used in this example are conventional methods known to those skilled in the art unless otherwise specified. The reagents and other materials used are all commercially available products unless otherwise specified. The germplasm resources used can all be obtained from national species The quality resource library is obtained.

[0023] 2. Method

[0024] 2.1 Extraction of wheat genomic DNA by SDS-Tris saturated phenol method

[0025] (1) Henong 825, Annong 0711, Zhoumai 23, Huapei 0616H-119, Jinnong 4, Gaoyou 9618, Ji 95-5219, Shimai 14, Xinmai 19, a total of 9 wheat varieties 2-3 seeds each, placed in a 2ml sterilized centrifuge tube, and ground into powder using a high-throughput tissue grinder. The above-mentioned wheat varieties can be purchased directly from the market or obtained from the National Germplasm Bank.

[0026] (2) Add 1.2 ml of DNA extraction buffer (200 mM Tris-Cl, 250 mM NaCl, 25 mM EDTA, 0.5% SDS, 2% β-ME).

[002...

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Abstract

The invention discloses a molecular marker for identifying wheatear sprouting resistance based on a TAMFT-3A gene and a method thereof. The TAMFT-3A gene has a nucleotide sequence as shown in SEQ ID NO.1, the molecular marker is an inserted / lost sequence which is positioned between 320 and 321 of the nucleotide sequence as shown in SEQ ID NO.1 and has a length of 33bp, and the nucleotide sequence of the molecular marker is as shown in the SEQ ID NO.2. The molecular marker is closely related to the wheatear sprouting resistance, and can be used for developing a novel functional marker for identifying the wheatear sprouting resistance, so that a new approach is provided to molecular identification of wheatear sprouting resistance, a new resource is provided to genetic breeding of wheatear sprouting resistance, the genetic resource library of wheatear sprouting resistance is enriched, and foundation is laid for genetic breeding of wheatear sprouting resistance.

Description

technical field [0001] The invention relates to the technical field of wheat molecular breeding, in particular to a molecular marker for identifying wheat ear germination resistance and a method thereof. Background technique [0002] Wheat is prone to ear germination (Pre-harvest sprouting, PHS) in continuous rainy weather before harvest. The yield of wheat with ear germination decreases, the quality deteriorates, and serious economic losses are caused to growers. (Groos et al., 2002; Chang et al., 2010). Wheat ear germination is affected by internal genetic factors (such as seed coat color, seed dormancy, amylase, ABA, GA, wheat ear shape, grain water absorption characteristics, etc.) and external environmental factors (such as light, temperature and humidity, etc.) (Zhu Yulei et al., 2014). Breeding wheat varieties with strong panicle germination resistance is an important breeding goal in rainy wheat areas. [0003] Wheat ear germination resistance belongs to the gene...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 赵良侠姜昊张海萍陈雪健朱玉磊王升星吴曾云曹佳佳刘凯张璨未文新李黎谢红咏常成卢杰马传喜
Owner ANHUI AGRICULTURAL UNIVERSITY
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