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SNP markers related to first delivery day age of South China Holstein cow, and application thereof

A technology for marking and dairy cows, applied in the field of molecular biology, can solve problems such as the long age of first birth in Holstein dairy cows, and achieve the effects of improving reproductive performance, increasing production efficiency, and enriching genetic resource pools

Pending Publication Date: 2021-04-16
WUHAN ACADEMY OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The embodiment of the present invention provides two SNP markers related to the age at first calving of Holstein dairy cows in southern China and their applications, so as to solve the problem of long age at first calving of Holstein dairy cows in southern China

Method used

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  • SNP markers related to first delivery day age of South China Holstein cow, and application thereof
  • SNP markers related to first delivery day age of South China Holstein cow, and application thereof
  • SNP markers related to first delivery day age of South China Holstein cow, and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1: Acquisition and identification of SNP markers closely related to reproductive traits

[0040] 1.1 Experimental population and phenotypic data

[0041] The first calving age records of 785 Holstein dairy cows in southern China were collected from a large-scale dairy farm in Wuhan, Hubei Province from 2015 to 2019. Blood was collected from the tail vein of each individual, anticoagulated with EDTA, and stored at -20°C for later use.

[0042] 1.2 Blood Genomic DNA Extraction

[0043] Use the TIANamp Blood DNAKit Blood Genomic DNA Extraction Kit to extract blood DNA. The specific steps are as follows:

[0044] (1) Take 200 μL of dairy cow whole blood in a 1.5 mL RNase-free EP tube, add 200 μL of buffer GB and 20 μL of Proteinase K premixed solution, fully invert and mix, and place at 56°C for 10 minutes, during which time invert and mix several times, the solution Become clear (if the solution does not become clear completely, prolong the lysis time until the ...

Embodiment 2

[0086] Example 2: Statistical Analysis

[0087]The genotype frequency and allele frequency of each locus were calculated by Excel, and the Hardy-Weinberg balance of each locus was analyzed by chi-square test. Use the SAS online program (https: / / welcome.oda.sas.com / ) GLM process to test the influence of genotype on primiparous age phenotype value, the model is as follows: Y=μ+G+e, where Y is an individual trait Phenotype value, μ is the population mean, G is the fixed effect of genotype, and e is the random error effect. Multiple comparisons of individual trait phenotype values ​​across genotypes were performed using the DUNCAN method.

[0088] 2.1 Statistical analysis results of primiparous age traits

[0089] The average age at first calving of 801 Holstein cows in southern China was 754.95 days, the standard deviation was 60.65 days, the maximum was 1153 days, and the minimum was 652 days.

[0090] 2.2 Genomic DNA detection and SNP typing

[0091] Cow blood DNA was detec...

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Abstract

The invention relates to two SNP markers related to the first birth day age of a South China Holstein cow, and application thereof. The rs42737928 site of the first SNP marker is located at the 113301213bp position of the No.3 chromosome of the South China Holstein cow genome, the base is C or G, and the first birth day age of the GG genotype individual of the first SNP marker is significantly smaller than that of the GC genotype individual; the rs43703011 site of the second SNP marker is located at the 85451298bp position of the sixth chromosome of the South China Holstein cow genome, the base is G or T, and the first birth day age of GG and TT genotype individuals of the second SNP marker is remarkably smaller than that of GT genotype individuals. The two SNP molecular markers are applied to combined breeding of Holstein cows in southern China, so that the accuracy of character selection of the first birth day age is greatly improved, dominant alleles and genotypes of the first birth day age of the cows can be quickly diffused in a cow group in advance, the generation interval is shortened, the breeding cost is reduced, The breeding process of dairy cows with high fecundity is accelerated, and the sustainable development of the dairy cow industry in China is promoted.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to two SNP markers related to the first calving age of Holstein dairy cows in southern China and applications thereof. Background technique [0002] The southern region of my country has a large population and a high demand for milk consumption, so it has great market potential in the development of the dairy industry. However, due to factors such as natural conditions and agricultural industrial structure, the southern milk supply rate is seriously insufficient. Molecular breeding technology based on contemporary genome science provides an important way to accelerate the progress of genetic selection in the southern Holstein dairy population, promote the overall benefit of the dairy industry in southern my country, and alleviate the imbalance between the supply and demand of dairy products in the region, and one of the important The premise is that effective genetic material that a...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12Q1/6869C12N15/11
Inventor 程蕾余婕刘辰晖胡修忠向敏夏瑜周源王定发陶弼菲
Owner WUHAN ACADEMY OF AGRI SCI
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