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Method for detecting polycyclic aromatic hydrocarbon content in smokeless tobacco products

A technology for smokeless tobacco and polycyclic aromatic hydrocarbons, applied to measuring devices, instruments, scientific instruments, etc., to achieve the effects of reducing pollution, improving analysis throughput, and reducing usage

Active Publication Date: 2017-05-10
YUNNAN TOBACCO QUALITY SUPERVISION MONITORING STATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] However, the tobacco industry currently does not have any standards to guide the detection of PAHs in smokeless tobacco products

Method used

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  • Method for detecting polycyclic aromatic hydrocarbon content in smokeless tobacco products
  • Method for detecting polycyclic aromatic hydrocarbon content in smokeless tobacco products
  • Method for detecting polycyclic aromatic hydrocarbon content in smokeless tobacco products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Weigh 3g of snus as sample A and place it in a 25mL ground-mouth Erlenmeyer flask, add 250 μL of cyclohexane solution containing deuterated naphthalene (320ng / mL) and deuterated benzo[a]pyrene (320ng / mL) as Add 15mL cyclohexane as the extractant for the internal standard substance; shake and extract the conical flask at room temperature for 40min, pass the extract through a 0.22μm nylon 66 filter membrane, collect the filtrate in the chromatographic bottle, and wait for GC-MS / MS analyze.

[0052] GC-MS / MS analysis conditions:

[0053] Chromatographic inlet temperature: 260°C; sample volume: 1μL; sample injection method: splitless injection; chromatographic column: HP-5MS UI (30m*0.32mm*0.25μm); temperature program: initial temperature 40°C, keep 7min; rise to 100°C at 20°C / min; rise to 300°C at 4°C / min and hold for 10min; transfer line temperature: 280°C; mass spectrometry ionization mode: EI; ion source temperature: 280°C; mass spectrometry scanning mode: dynamic mult...

Embodiment 2

[0073] Repeat Example 1, with the following differences:

[0074] Weigh 10g of chewing tobacco as sample B and place it in a 100mL ground-necked Erlenmeyer flask, add 500 μL of methanol solution containing deuterated benzo[a]anthracene (200ng / mL) and deuterated phenanthrene (200ng / mL) as an internal standard substance , and then add 50mL of methanol as the extractant; shake the Erlenmeyer flask at room temperature for 60min, pass the extract through a 0.45μm polyethersulfone (PES) filter membrane, collect the filtrate in the chromatographic flask, and wait for GC-MS / MS analysis .

[0075] GC-MS / MS analysis conditions:

[0076] Chromatographic inlet temperature: 260°C; sample volume: 1μL; sample injection method: splitless injection; chromatographic column: DB-5MS capillary column (30m*0.25mm*0.25μm); temperature program: initial temperature 150°C , rise to 280°C at 5°C / min and hold for 10min; transfer line temperature: 280°C; mass spectrometry ionization mode: EI; ion source...

Embodiment 3

[0081] Repeat Example 1, with the following differences:

[0082] Weigh 10g of snuff as sample C and place it in a 50mL ground-mouth Erlenmeyer flask, add 500 μL of dichloromethane solution containing deuterated benzo[a]pyrene (320ng / mL) as an internal standard substance, and then add 20mL of dichloromethane as Extractant: Shake the Erlenmeyer flask at room temperature for 60 minutes, pass the extract through a 0.22 μm polyethersulfone (PES) filter membrane, collect the filtrate in a chromatographic bottle, and wait for GC-MS / MS analysis.

[0083] GC-MS / MS analysis conditions:

[0084] Chromatographic inlet temperature: 260°C; sample volume: 1μL; sample injection method: splitless injection; chromatographic column: HP-5MS capillary column (30m*0.25mm*0.25μm); temperature program: initial temperature 180°C , raised to 270°C at 3°C / min and kept for 20min; transfer line temperature: 280°C; mass spectrometry ionization mode: EI; ion source temperature: 250°C; mass spectrometry sc...

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Abstract

The invention discloses a method for detecting polycyclic aromatic hydrocarbon content in smokeless tobacco products. The method includes the steps: extracting the smokeless tobacco products by the aid of extraction agents; detecting filtered extraction solution by the aid of a GC-MS / MS (gas chromatography-mass spectrometry). The method can simultaneously measure content of indene, naphthalene, 1-methylnaphthalene, 2-methylnaphthalene, acenaphthylene, acenaphthene, fluorine, phenanthrene, anthracene, fluoranthene, pyrene, benzo [a] anthracene, chrysene, benzo [b] fluoranthene, benzo [k] fluoranthene, benzo [j] fluoranthene, benzo [e] pyrene, benzo [a] pyrene, perylene, indene [1, 2, 3-cd] pyrene, diphenyl [a, h] anthracene, benzo [g, h, i] pyrene and the like in the smokeless tobacco products. The method fills in gaps of the prior art and has the advantages that the method is simple, convenient and rapid in sample pretreatment, goof in detection accuracy and repeatability and high in analytical test flux, and the method is applicable to rapid analysis of large-batch samples.

Description

technical field [0001] The invention belongs to the technical field of analysis and detection of smokeless tobacco products, and specifically relates to a method capable of simultaneously detecting 22 kinds of polycyclic aromatic hydrocarbons in smokeless tobacco products, including: indene, naphthalene, 1-methylnaphthalene, 2-methylnaphthalene Naphthalene, acenaphthylene, acenaphthene, fluorene, phenanthrene, anthracene, fluoranthene, pyrene, benzo[a]anthracene, chrysene, benzo[b]fluoranthene, benzo[k]fluoranthene, benzo[j]fluoranthene Anthracene, Benzo[e]pyrene, Benzo[a]pyrene, Perylene, Indeno[1,2,3-cd]pyrene, Dibenzo[a,h]anthracene, Benzo[g,h,i] Pyrene content method. [0002] technical background [0003] Smokeless tobacco products (STPs) refer to tobacco products that do not need to be burned or produce smoke when used, and can be divided into oral type (Oral STPs) and nasal type (Nasal STPs) according to the way of use Types of. Oral STPs include chewing tobacco, va...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 蔡洁云李雪梅陈新瑞王惠平张庆刚刘冰王淑华李海燕马慧宇肖燕
Owner YUNNAN TOBACCO QUALITY SUPERVISION MONITORING STATION
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