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Method for increasing yield of pachymic acid of poria cocos liquid fermented mycelium

A technology of liquid fermentation and mycelium, applied in the directions of microorganism-based methods, fermentation, biochemical equipment and methods, etc., can solve the problems of speeding up industrial production, long fermentation period, disadvantages, etc., and achieve the effect of increasing yield and increasing content

Active Publication Date: 2017-05-17
HUAIHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its fermentation period is long, which is not conducive to accelerating industrial production

Method used

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  • Method for increasing yield of pachymic acid of poria cocos liquid fermented mycelium
  • Method for increasing yield of pachymic acid of poria cocos liquid fermented mycelium

Examples

Experimental program
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Effect test

Embodiment 1

[0077] Isolation and Identification of Poria cocos Strain ZJ2

[0078] The sclerotium of Poria cocos was obtained from the planting base of Poria cocos in Jingzhou County, Hunan Province in March 2011. The sclerotia was separated and purified by the method of tissue separation after surface disinfection, and an original strain of Poria cocos was obtained, which was inserted into PDA slant medium to prepare slant mother species , the slanted mother species is inoculated into the solid culture medium again to obtain the Poria cocos fruiting body, and the basidiospores in the Poria cocos fruiting body have been cultivated to obtain a bacterial strain with high content of pachymic acid, which is the Poria cocos (Poria cocos) bacterial strain used in the present invention ZJ2.

[0079] Poria cocos (Poria cocos) strain ZJ2 described in the present invention is identified through molecular biology techniques, including performing ITS-PCR amplification and sequencing, and the obtained...

Embodiment 2

[0081] A kind of liquid seed culture medium for Poria cocos fermentation, in every liter, it is composed of:

[0082] Potato 150g, glucose 10g, cellulose 10g, masson pine root extract 20g, glycerin 3g, (NH 4 ) 2 SO 4 3g, MgSO 4 ·7H 2 O 1.5g, KH 2 PO 4 2g, 0.1g of complex vitamin B, and the rest is water; and adjust the pH to 6.6.

[0083] Masson pine extract is prepared by the following method:

[0084] Wash the roots of Pinus massoniana and put them in a drying oven, dry them at 45°C to constant weight, crush them, and pass through a 30-mesh sieve; weigh 20g of Pinus massoniana root powder into a container, add 3 times the volume of distilled water, and heat to boil , decocted on low heat for 30 minutes, and filtered through gauze to remove the precipitate; after the filtrate was centrifuged at 8000r / min for 15 minutes, the supernatant was taken to obtain the extract of Pine massoniana.

[0085] The cellulose is food-grade microcrystalline cellulose with a particle ...

Embodiment 3

[0087] A kind of liquid seed culture medium for Poria cocos fermentation, in every liter, it is composed of:

[0088] Potato 180g, glucose 8g, cellulose 7g, masson pine root extract 25g, glycerin 2g, (NH 4 ) 2 SO 4 2.5g, MgSO 4 ·7H 2 O 1.8g, KH 2 PO 4 3g, 0.2g of complex vitamin B, and the rest is water; and adjust the pH to 7.

[0089] Wherein the preparation method of the root extract of Pinus massoniana is the same as in Example 2, only the root powder of Pinus massoniana needs to be adjusted to 25g; the selection of cellulose and vitamin B complex is the same as in Example 2.

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Abstract

The invention relates to a method for increasing the yield of pachymic acid of poria cocos liquid fermented mycelium. The method comprises the steps of performing liquid fermentation on the a poria cocos bacterial strain ZJ2, and adding a masson pine extract, cellulose and composite vitamin B into a liquid seed culture medium and / or a liquid fermentation culture medium for poria cocos fermentation. By the method provided by the invention, the yield of the poria cocos mycelium can be optimized and maximized, the pachymic acid content of the mycelium is increased, industrialized production of the mycelium can be realized, the problem of damage to wild pine forest resource caused by the fact that poria cocos sclerotium is cultivated by pine tree pockets is solved, and foundation is laid and technical guarantee is provided for comprehensive development and industrialized production of the medical and edible value of the poria cocos ; the method is simple in process, effective and short in fermentation cycle, has application value in industrialized production, and can successfully fulfill the aim of obviously increasing the yield of the expression products.

Description

technical field [0001] The invention relates to the field of liquid fermentation of medicinal fungi, in particular to a method for increasing the output of pachymic acid in the liquid fermentation mycelium of Poria cocos. Background technique [0002] Poria cocos is a medicinal fungus, which not only has the function of medical care, but also can be eaten. It is a traditional Chinese medicinal material and a food with a long history in my country. Poria cocos is sweet, light and flat in taste, and has the effects of diuresis and dampness, invigorating the spleen and invigorating the middle, calming the mind and calming the nerves. Poria cocos acid is a unique tetracyclic triterpene compound of Poria cocos and one of the main active ingredients of Poria cocos. It has a variety of pharmacological activities related to drug efficacy. According to research reports, the content of pachymic acid in dried Poria cocos sclerotium is 0.1462%-0.2996%. Pachynic acid has anti-tumor, an...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P33/00C12R1/645
CPCC12P33/00C12N1/145C12R2001/645
Inventor 邹娟胡兴刘胜贵彭程吴清旻
Owner HUAIHUA UNIV
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