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A kind of improved fermentation method of bacterial cellulose

A technology of bacterial cellulose and fermentation methods, applied in the direction of microbial-based methods, fermentation, biochemical equipment and methods, etc., can solve the problems of insufficient composite degree, small pore size, easy desorption, etc., to avoid fiber breakage, increase Disturbance degree, effect of water holding capacity reduction

Active Publication Date: 2020-07-31
南京荣之盛生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the synthesized biological cellulose has a single structure and relatively small pore size, and its application is limited. Generally, it is modified by loading compounds with specific chemical properties on the surface of the cellulose in the later stage, but the degree of recombination is not enough, and it is easy to desorption problem
Document 1 (Luo Chengcheng, Advances in Modification and Application of Cellulose. 2015:767-773) summarizes three types of methods for modifying cellulose, namely physical modification, chemical modification and biological modification. Both obtain the finished cellulose product first, and then carry out post-modification on it. This type of modification will easily cause the change of the crystal morphology of cellulose, making it lose the original high crystallinity, high strength and other excellent properties of cellulose.
Document 2 (Keshk S, etc.. Influence of lignosulfonate on crystal structure and productivity of bacterial cellulosein a static culture, 2006,40 (1): 4-8) reported a kind of lignosulfonate by adding lignosulfonate in the fermented liquid The method is to modify bacterial cellulose. This method will produce certain cellulose faults while improving the binding force between cellulose fibers, which will affect the size of the final modified product and limit the application.
Document 3 (Zhao Yanfeng. Cellulose Modification Technology and Progress. 2006,20(2):11-14) uses cellulase to treat bacterial cellulose, although the binding force between cellulose is improved, but cellulase will degrade Cellulose chain

Method used

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  • A kind of improved fermentation method of bacterial cellulose
  • A kind of improved fermentation method of bacterial cellulose
  • A kind of improved fermentation method of bacterial cellulose

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preparation example Construction

[0020] The preparation of seed liquid among the present invention and parameter monitoring can refer to existing method, and wherein seed liquid can be made through the following steps:

[0021] The strains preserved at 4°C were kept at 30°C for 20 minutes, and a ring of strains was picked up with an inoculation loop and streaked on the solid plate medium, and the plate was cultured in a 30°C incubator for 36 hours. Pick 2-3 loops of the activated seeds with an inoculation loop, inoculate them into a 500mL Erlenmeyer flask containing 100mL of seed solution, and then put them into a shaker and culture them with reciprocating shaking at 120-160rpm for 48h.

[0022] Composition of solid medium (g / 100mL): glucose 2.0, sucrose 1.0, magnesium sulfate 0.04, citric acid 0.11, sodium dihydrogen phosphate 0.25, peptone 1.0, agar 1.8, yeast extract powder 0.1. pH=6.0. Sterilize at 121°C for 30min.

[0023] Seed solution composition (g / 100mL): glucose 2.0, ammonium sulfate 0.6, potassium...

Embodiment 1

[0033] The seed solution was inoculated into a 500mL Erlenmeyer flask containing 100mL fermentation broth, and the dynamic culture was carried out at 30°C for 72h at 160 rpm. After 20 hours of fermentation, different interference factors were added to interfere with the entire bacterial cellulose production and fermentation process. The amount of different interfering factors is: dithiothreitol 10mg, chloramphenicol 5mg, Tween-80 400mg, emulsifier OP-10 400mg, quartz sand 1500mg, nano-silica 300mg, the corresponding modified fermentation product bacteria The celluloses are BC-A, BC-B, BC-C, BC-D, BC-E and BC-F.

[0034] figure 1 It is the scanning electron micrograph of bacterial cellulose modified by adding interfering factors and without adding interfering factors, a is dithiothreitol, b is chloramphenicol, c is Tween-80, d is emulsifier OP- 10. e is quartz sand, f is nano silicon dioxide, and g is bacterial cellulose fermented without adding interfering factors. Depend o...

Embodiment 2

[0036]The seed solution was inoculated into a 500mL Erlenmeyer flask containing 100mL fermentation broth, and the dynamic culture was carried out at 30°C for 72h at 160 rpm. After 35 hours of fermentation, different interference factors were added to interfere with the entire bacterial cellulose production and fermentation process. The amounts of different interfering factors are: dithiothreitol 15mg, chloramphenicol 10mg, Tween-80 500mg, emulsifier OP-10 500mg, quartz sand 2000mg, nano silicon dioxide 400mg, the corresponding modified fermentation product bacteria The celluloses are BC-A, BC-B, BC-C, BC-D, BC-E and BC-F.

[0037] figure 2 It is the XRD pattern of the product bacterial cellulose modified by adding interfering factors and without adding interfering factors. pass figure 2 , calculate the crystallization index of cellulose interfered by different interference factors, the results are shown in Table 1. It can be seen from Table 1 that, except that the surfac...

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Abstract

The invention discloses an improved fermentation method of bacterial cellulose. According to the method, in a fermentation process of acetobacter xylinum, different interference factors are added to regulate the fermentation process, so that the microstructure and water-holding capacity of acetobacter xylinum are changed. With respect to the water-holding capacity, tween-80 can increase the water-holding capacity of bacterial cellulose from 90 times of the weight of itself to 105 times of the weight of itself; dithiothreitol and OP-10 taken as an emulsifier can slightly reduce the water-holding capacity of bacterial cellulose; and chloramphenicol and nanometer silica can increase the water-holding capacity to 70 times of the weight of bacterial cellulose itself. Based on practical application requirements, different interference factors can be added in the fermentation process of bacterial cellulose, the original crystal form of cellulose is guaranteed, and the microstructure and water-holding capacity of the final bacterial cellulose product are regulated, thereby developing later application of bacterial cellulose.

Description

technical field [0001] The invention relates to an improved fermentation method of bacterial cellulose, which belongs to the field of biotechnology. Background technique [0002] Bacterial cellulose (BC) is a bacterial secretion, which is formed by the simple condensation of glucose, and has a high fiber content and a high degree of polymerization. At present, the synthesized biological cellulose has a single structure and relatively small pore size, and its application is limited. Generally, it is modified by loading compounds with specific chemical properties on the surface of the cellulose in the later stage, but the degree of recombination is not enough, and it is easy to detachment problem. Document 1 (Luo Chengcheng, Advances in Modification and Application of Cellulose. 2015:767-773) summarizes three types of methods for modifying cellulose, namely physical modification, chemical modification and biological modification. Both obtain the finished cellulose product fi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/04C12R1/02
CPCC12P19/04
Inventor 顾焱梁光芸陈春涛
Owner 南京荣之盛生物科技有限公司
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