Probiotics composition for alleviating or improving pelvic inflammatory diseases and preparation method thereof
A technology of probiotics and compositions, applied in the field of biomedicine, can solve problems such as damage to yang, achieve the effects of promoting development, improving immunity, and alleviating or improving pelvic inflammatory disease
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[0055] The preparation method of the traditional Chinese medicine extract of the same medicine and food is as follows:
[0056] Soak angelica, peach kernel, raw hawthorn, poria cocos, cinnamon, licorice, rose, dandelion, and houttuynia in 8-10 times the water for 30-60 minutes, decoct, and continue decocting for 20-40 minutes after the water is boiled, and filter to obtain the first The first filtrate; the dregs are decocted with 6-8 times the water for the second time, and the water is boiled for 20-40 minutes, and the second filtrate is filtered and mixed with the first filtrate, concentrated to a solid content of more than 30%, and sprayed Dried, pulverized, and sieved to obtain the traditional Chinese medicine extract with the same source of medicine and food.
[0057] As a preferred embodiment, in the above-mentioned probiotic traditional Chinese medicine preparation, the weight ratio of the probiotic composition to the medicinal and edible traditional Chinese medicine ex...
Embodiment 1
[0072]Embodiment 1: the preparation of the lyophilized powder of probiotics
[0073] 1) Three strains of Lactobacillus (Lactobacillus acidophilus BLCC2-0024 with preservation number CCTCC NO: M2015129, Lactobacillus plantarum LP and Lactobacillus rhamnosus with preservation number CCTCC M2010150) were respectively activated by medium Afterwards, it was inoculated in the fermentation medium, and cultured at a constant temperature of 37°C, so that the bacterial concentration reached 4.0×10 in the logarithmic growth phase. 9 -8.0×10 9 cfu / ml;
[0074] Preparation of Lactobacillus seed solution: Take out the strain preservation tube, streak with lactic acid bacteria slant solid medium for recovery, and cultivate at 37 degrees for 24-36 hours. Pick the colonies on the slant culture medium for crystal violet staining and microscopic examination, and they are short bacilli or long bacilli. Pick the colony and inoculate it into 100mL lactic acid bacteria medium, and cultivate it in...
Embodiment 2
[0078] Embodiment 2: the preparation of medicine and food homologous traditional Chinese medicine extract
[0079] 1) According to the preferred parts by weight: angelica 2g, peach kernel 3g, raw hawthorn 8g, poria cocos 10g, cinnamon 2g, licorice 6g, rose 6g, dandelion 6g, houttuynia cordata 15g;
[0080] 2) Soak the mixture in 10 times the water for 40 minutes, decoct, boil the water for 30 minutes, and filter to obtain the first filtrate; decoct the dregs with 8 times the water for the second time, boil the water for 30 minutes, filter The obtained second filtrate is mixed evenly with the first filtrate, and the traditional Chinese medicine liquid is concentrated by a vacuum decompression rotary evaporator until the solid content is more than 30%, and then enters the spray dryer, the inlet air temperature is controlled at 140°C, and the outlet air temperature is 90°C. The rotational speed of the atomizer is 45 rev / min, and the feeding speed is 5ml / min to obtain a spray-drie...
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