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Method for inducing castanea mollissima 2n pollen based on inflorescence dip

A technology for inflorescence and chestnut, applied in the field of polyploidy induced by chemical agents, can solve the problems of low 2n pollen ratio and inability to directly apply breeding practices

Pending Publication Date: 2017-05-31
CHANGLI INST OF POMOLOGY HEBEI ACADEMY OF AGRI & FORESTRY SCI
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In order to overcome the deficiency that the ratio of 2n pollen in chestnut natural pollen is too low and cannot be directly applied to breeding practice, and make up for the blank of artificial induction method of chestnut 2n pollen with large frequency, the present invention aims to provide a method for inducing chestnut 2n pollen based on inflorescence dipping. method can obtain large frequency 2n pollen

Method used

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  • Method for inducing castanea mollissima 2n pollen based on inflorescence dip
  • Method for inducing castanea mollissima 2n pollen based on inflorescence dip
  • Method for inducing castanea mollissima 2n pollen based on inflorescence dip

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Embodiment 2～10

[0061] In the following examples, a total of 10 chestnut varieties with similar florescences were carried out based on "Yankui", X44-21, Yanming, Nanchui No. 5, Timazhu, Yanchang, Yanhong, Yanlong, Yanfeng, and Huaijiu. Inflorescence dipping induced 2n pollen test of chestnut.

[0062] The chestnut 2n pollen induction operation was carried out according to the following steps:

[0063] 1) Select induction material

[0064] Inflorescences on male branches of 10 chestnut varieties including Yankui, X44-21, Yanming, Nanchui No. 5, Timazhu, Yanchang, Yanhong, Yanlong, D7-43 and Huaijiu were selected as induction treatment materials.

[0065] 2) Preparation of induction agents

[0066] Prepare the required inducing agents [0.75% (W / V) colchicine, 0.1% (V / V) dimethyl sulfoxide] 2 days before using the inducing agents to treat the inflorescences, and place them in a refrigerator at 4°C for use.

[0067] 3) Impregnating inflorescences with inducing agents

[0068] Select the healt...

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Abstract

The invention belongs to the technical field of fruit tree breeding, and provides a method for inducing castanea mollissima 2n pollen based on inflorescence dip. The method comprises the following steps: (1) selecting an inducing material; (2) preparing an inducing agent; (3) dipping inflorescence by using the inducing agent; (4) bagging the inflorescence before pollen is scattered; and (5) collecting the pollen: when the inflorescence in a paper bag is in a male flower full-blossom period, picking the paper bag and the inflorescence, and collecting the naturally scattered pollen in the bag. The castanea mollissima inflorescence is used as an object, the inducing agent, dosage, time, part and other factors suitable for the castanea mollissima inflorescence are selected, a castanea mollissima 2n pollen induction technology based on the inflorescence dip is created, high-frequency 2n pollen can be obtained, and a feasible castanea mollissima 2n pollen induction method is provided for the future, so that the technical support is provided for the successful ploidy breeding of the castanea mollissima.

Description

technical field [0001] The invention belongs to the technical field of fruit tree breeding, and in particular relates to a method for inducing polyploids with chemical agents. Background technique [0002] Chestnut is native to China and has a long history of cultivation. It is an excellent dried fruit tree species. More than two-thirds of the planting area of ​​chestnuts in China is located in the economically underdeveloped and poor mountainous areas. With the development of history, a unique mountain chestnut industry has gradually formed, which has become an important economic dependence for farmers in mountainous areas to become rich. Variety is an important guarantee for the healthy development of an industry, and it is also an eternal basic demand. At present, a variety of methods are used at home and abroad to cultivate new varieties for production services. The main ways to obtain new varieties on plants include: seed selection, bud mutation selection, hybrid breed...

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Application Information

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IPC IPC(8): A01H1/08
CPCA01H1/08
Inventor 王广鹏李颖张树航郭燕张馨方于秋香
Owner CHANGLI INST OF POMOLOGY HEBEI ACADEMY OF AGRI & FORESTRY SCI
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