Preparation method for fermenting and producing bacteriocin rice feed rich in taurine
A technology of taurine and rice, applied in the field of agriculture and animal husbandry, can solve problems such as slow growth of fish, achieve normal visual function, promote full absorption, and regulate nerve conduction.
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Embodiment 1
[0017] Embodiment 1: For fermenting and producing bacteriocin rich in taurine, the rice feed comprises the following raw materials in parts by mass: rice: 40 parts, soybean meal: 20 parts, bran: 20 parts, cysteine: 5 parts, Methionine 5 parts.
[0018] Be used to ferment and produce the bacteriocin rice feed preparation step that is rich in taurine: step 1: the preparation of potato culture medium: be potato by mass ratio: the ratio of water=1:6 prepares potato juice, after adding potato to water, Boil for 25 minutes, filter and remove slag to obtain potato juice, then add 2% glucose by volume of potato juice to make potato medium liquid;
[0019] Step 2. Activation and cultivation of strains: Add 20mL of the potato medium liquid prepared in step 1 to the activated strains of Bacillus subtilis YS-45, Saccharomyces S-78 and Aspergillus niger PL-39 respectively to make a bacterial suspension , and then the bacteria suspension, respectively, under 250r / min shaker, 32 ℃ under the...
Embodiment 2
[0023] Embodiment 2: For fermenting and producing bacteriocin rich in taurine, the rice feed comprises the following raw materials in parts by mass: rice: 42 parts, soybean meal: 20 parts, bran: 20 parts, cysteine: 4 parts, Methionine 4 parts.
[0024] Be used to ferment and produce the bacteriocin rice feed preparation step that is rich in taurine: step 1, the preparation of potato culture medium: be potato by mass ratio: the ratio of water=1:4 prepares potato juice, after adding potato to water, Boil for 35 minutes, filter to remove slag to obtain potato juice, then add 2% glucose by volume of potato juice to make potato medium liquid;
[0025] Step 2. Activation and cultivation of strains: Add 15ml of the potato medium liquid prepared in step 1 to the activated strains of Bacillus subtilis YS-45, Saccharomyces S-78, and Aspergillus niger PL-39 respectively to make a bacterial suspension. Then, the bacterial suspension was expanded and cultivated under 250r / min shaker at 30...
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