Application of pleurotus ferulae polysaccharide (PFPS) in preparing dendritic cell vaccine adjuvant

A dendritic cell and vaccine adjuvant technology, which is applied in the application field of ferulic mushroom polysaccharide as an adjuvant for preparing dendritic cell vaccine, can solve the problems of unsuitability for clinical application, toxic and side effects of the adjuvant and the like

Active Publication Date: 2017-05-31
新疆鼎聚医学检验所有限公司
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Problems solved by technology

However, most adjuvants have toxic side eff...

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  • Application of pleurotus ferulae polysaccharide (PFPS) in preparing dendritic cell vaccine adjuvant
  • Application of pleurotus ferulae polysaccharide (PFPS) in preparing dendritic cell vaccine adjuvant
  • Application of pleurotus ferulae polysaccharide (PFPS) in preparing dendritic cell vaccine adjuvant

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Embodiment Construction

[0023] The preferred embodiments of the present invention will be described below in conjunction with the accompanying drawings. It should be understood that the preferred embodiments described here are only used to illustrate and explain the present invention, and are not intended to limit the present invention.

[0024] Extraction and Purification of Polysaccharides from Mushroom Ferulae

[0025] Weigh 100g lyophilized powder of ferulae ferulae, extract with 1 L of 95% ethanol for 2 hours to remove fat, centrifuge at 5000 rpm / min for 10 min to collect the precipitate, resuspend the precipitate in 1 L of deionized water, bathe in 60°C for 2 hours, and ultrasonicate 20min to aid dissolution, 6000rpm / min centrifuge for 15min, collect the supernatant, after repeated leaching of the filter residue 4 times, combine the supernatant, and concentrate it to 1 / 10 volume by vacuum rotation at 60℃ water bath temperature, then use the Sevage reagent method: press Concentrate: Sevage reage...

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Abstract

The invention discloses an application of pleurotus ferulae polysaccharide (PFPS) in preparing a dendritic cell vaccine adjuvant. The PFPS has an effect of stimulating dendritic cell maturation so as to promote the immunoreaction of dendritic cell vaccines and improve the anti-tumor effect.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to the application of asafoetida polysaccharide as an adjuvant for preparing dendritic cell vaccines. Background technique [0002] Dendritic cells (DC) are professional antigen-presenting cells in the body, which can recognize, capture and process antigens, present antigens to naïve T cells, activate antigen-specific immune responses, and connect the innate immune system and the acquired immune system. During antigen presentation, different signals are involved in the activation of antigen-specific T cells, including MHC-polypeptide complexes, interactions between costimulatory molecules on the surface of DCs and T cells, and cytokines secreted by DCs. Interleukin-12 (IL-12) secreted by DCs can promote CD4 + Th1 type immune response and cytotoxic T lymphocyte response, these two immune responses play an important role in tumor therapy. DC vaccine (DC-based vaccine) is a prom...

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Application Information

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IPC IPC(8): A61K39/39A61K39/00A61P35/00C08B37/00
CPCA61K39/0005A61K39/39A61K2039/5154A61K2039/55583C08B37/0003
Inventor 李金耀李金玉袁鹏飞阿地拉·艾皮热
Owner 新疆鼎聚医学检验所有限公司
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