Application of pleurotus ferulae polysaccharide (PFPS) in preparing dendritic cell vaccine adjuvant
A dendritic cell and vaccine adjuvant technology, which is applied in the application field of ferulic mushroom polysaccharide as an adjuvant for preparing dendritic cell vaccine, can solve the problems of unsuitability for clinical application, toxic and side effects of the adjuvant and the like
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[0023] The preferred embodiments of the present invention will be described below in conjunction with the accompanying drawings. It should be understood that the preferred embodiments described here are only used to illustrate and explain the present invention, and are not intended to limit the present invention.
[0024] Extraction and Purification of Polysaccharides from Mushroom Ferulae
[0025] Weigh 100g lyophilized powder of ferulae ferulae, extract with 1 L of 95% ethanol for 2 hours to remove fat, centrifuge at 5000 rpm / min for 10 min to collect the precipitate, resuspend the precipitate in 1 L of deionized water, bathe in 60°C for 2 hours, and ultrasonicate 20min to aid dissolution, 6000rpm / min centrifuge for 15min, collect the supernatant, after repeated leaching of the filter residue 4 times, combine the supernatant, and concentrate it to 1 / 10 volume by vacuum rotation at 60℃ water bath temperature, then use the Sevage reagent method: press Concentrate: Sevage reage...
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