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Preparation of rhamnooligosaccharide derived from green algae and its application in cosmetics

A technology of rhamnooligosaccharides and cosmetics, applied in the field of biotechnology applications, to achieve the effects of mild reaction conditions, easy absorption, and simple methods

Active Publication Date: 2021-03-12
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After consulting relevant literature and books, there is currently no relevant report on the preparation of rhamnose and its oligosaccharides from green algae by using multi-enzyme coupling regulation, and using them as functional ingredients in cosmetics

Method used

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  • Preparation of rhamnooligosaccharide derived from green algae and its application in cosmetics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The preparation method of rhamnooligosaccharide is as follows.

[0026] (1) Extraction of Chlorella polysaccharide: add water to the washed, dried and crushed Enteromorpha powder according to the ratio of material to liquid 1:30, extract at 95-100°C for 3 hours, and centrifuge while hot to obtain the supernatant, which is the Chlorella polysaccharide solution .

[0027] (2) Preparation of rhamnosan: adjust the pH of the chlorophyll polysaccharide solution to 8.5, place it at -20°C for 18-24 hours, thaw it at room temperature, and pass it through a 300-mesh sieve to obtain insoluble matter. Add double ethanol to the insoluble matter, centrifuge, and redissolve with water to obtain rhamnosan solution.

[0028] (3) Enzymatic hydrolysis: add polysaccharide degrading enzyme with an enzyme activity of 200 IU / ml to the rhamnosan solution, the amount of enzyme added is 5% (v / v), and Mg 2+ , at 40°C, pH 7.5, enzymatic hydrolysis for 0.5 h; after enzymatic hydrolysis, heat to b...

Embodiment 2

[0032] The preparation method of rhamnooligosaccharide is as follows.

[0033] (1) Extraction of Chlorella polysaccharide: add water to the washed, dried and crushed Enteromorpha powder according to the ratio of material to liquid 1:30, extract at 95-100°C for 3 hours, and centrifuge while hot to obtain the supernatant, which is the Chlorella polysaccharide solution .

[0034] (2) Preparation of rhamnosan: adjust the pH of the chlorophyll polysaccharide solution to 8.5, place it at -20°C for 18-24 hours, thaw it at room temperature, and pass it through a 300-mesh sieve to obtain insoluble matter. Add double ethanol to the insoluble matter, centrifuge, and redissolve with water to obtain rhamnosan solution.

[0035] (3) Enzymatic hydrolysis: add polysaccharide degrading enzyme with an enzyme activity of 300 IU / ml to rhamnosan solution, the amount of enzyme added is 4% (v / v), and Mg 2+ , at 45°C, pH 8.0, enzymatic hydrolysis for 1 h; after the end of the enzymatic hydrolysis, ...

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Abstract

The invention relates to a preparation method of rhamnooligosaccharide and its application in cosmetics. The preparation steps include the preparation, classification, biotransformation and regulation of the green algae polysaccharide to obtain the final degradation product. The method is simple in operation, mild in reaction, and can efficiently obtain rhamnooligosaccharides derived from green algae. The obtained product can increase the bound water ratio of the stratum corneum of the skin and prevent skin moisture loss. In addition, it also has the effect of anti-oxidation, can slow down the relaxation of the dermis, and achieve the function of preventing skin aging by improving skin metabolism.

Description

technical field [0001] The invention relates to a method for preparing rhamnooligosaccharide derived from green algae and its application in cosmetics, in particular to a method for preparing rhamnooligosaccharide, and belongs to the field of biotechnology applications. Background technique [0002] As we grow older, the collagen that maintains skin elasticity and plays a deep moisturizing role begins to decrease, and the activity of skin proteins begins to decrease, resulting in a decrease in the moisture content in the stratum corneum of the skin, thereby reducing the softness and elasticity of the skin. In order to solve this problem, most of the cosmetics sold on the market achieve the purpose of moisturizing the skin by adding hydrophilic moisturizing ingredients. At present, the more commonly used moisturizing ingredients in cosmetics include polyols (propylene glycol, etc.), polysaccharides (hyaluronic acid, etc.), and ceramides. The latest research has found that rh...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/04C12P19/14A61K8/60A61Q19/00A61Q19/08
CPCA61K8/60A61Q19/00A61Q19/08C12P19/04C12P19/14
Inventor 王鹏崔洁芬李银平
Owner OCEAN UNIV OF CHINA
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