Method for detecting trace amount of aldosterone in plasma by using high-resolution mass spectrometer

Abstract

The invention relates to a method for detecting a trace amount of aldosterone in plasma by using a high-resolution mass spectrometer. The problem that the trace amount of aldosterone in the plasma cannot be detected by a mass spectrometer can be solved effectively. The method comprises the following steps: preparing a standard working solution and an internal standard working solution; adding a plasma sample in the internal standard working solution, mixing evenly, then adding a protein precipitation solution, carrying out eddying and centrifuging, enabling supernate to flow through an activated HLB solid-phase extracting column, carrying elution with methanol, collecting elution liquid, drying to obtain an extract, redissolving the extract with a methanol aqueous solution, filtering to obtain filtrate, and detecting the filtrate by using a liquid chromatography-mass spectrometer; setting detecting conditions of the liquid chromatography-mass spectrometer, comparing the result, which is measured by a ThermoFisher QE mass spectrometer, of the plasma sample with the standard curve of an aldosterone standard working solution of six working points with known concentration so as to detect the trace amount of aldosterone in plasma. The method is simple, is easy to operate, accurate in detection and good in effect, and has quite high actual application value and remarkable economic benefit and social benefit.

Description

technical field [0001] The invention relates to a detection method, in particular to a method for detecting trace aldosterone in plasma by using a high-resolution mass spectrometer. Background technique [0002] Aldosterone is a steroid hormone synthesized and secreted by the zona globular cells of the adrenal cortex and belongs to the mineralocorticoid family. It has three main functions: 1) It binds to mineralocorticoid receptors in the principal cells of the distal tubule and urinary collecting duct, increases the permeability of the luminal membrane to potassium ions and sodium ions, and activates Na+ / K+ on the basal side pump, increasing the reabsorption of sodium ions and water while excreting more potassium ions into the urine. 2) Stimulate the secretion of hydrogen ions from the urinary collecting duct, regulate the concentration of bicarbonate in the plasma, and maintain the acid-base balance. 3) Act on the central nervous system through the posterior pituitary gl...

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Problems solved by technology

[0009] In view of the above situation, in order to overcome the defects of the prior art, the object of the present invention is to provide a method for detecting trace al

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