Method for increasing content of siamenoside I in momordica grosvenori

A kind of technology of Luo Han Guo, siamen glycosides

Inactive Publication Date: 2017-06-06
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, simonoside I only exists in the pulp that accounts for less than 15% of the fruit weight, and the content is extremely low, so that the price is expensive, which greatly limits its wide application in ...

Method used

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  • Method for increasing content of siamenoside I in momordica grosvenori

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Experimental program
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Effect test

Embodiment 1

[0027] Step 1, be that 2% ethanol aqueous solution is dissolved methyl jasmonate with volume fraction, be mixed with the ethanol mother liquor that concentration is 200mmol / L methyl jasmonate, and sterilize with the microporous membrane of 0.22 μm, the jasmine after sterilization Add the ethanol mother liquor of methyl jasmonate to MS solid medium, the amount of methyl jasmonate added is 200μmol / L, sterilize and cool to 24~26℃, wherein, MS solid medium includes: MS, 1.5mg / L L 6-benzylaminoadenine, 0.3mg / L indolebutyric acid, 3.5g / L agar, 30g / L sucrose, and 1.0g / L activated carbon;

[0028] Step 2. Inoculate the Luo Han Guo tissue cultured seedlings in the MS solid medium containing methyl jasmonate obtained in step 1, place them in a relative humidity of 60-66%, light intensity of 1300-1500Lux, light time of 8h / d, temperature of 21~ After culturing at 25°C for 30 days, the tissue-cultured seedlings were transplanted to the field for conventional field cultivation.

Embodiment 2

[0030] Dissolve methyl jasmonate in an aqueous ethanol solution with a volume fraction of 2%, and prepare a methyl jasmonate solution with a concentration of 200 μmol / L. After 25 days of pollination of Luo Han Guo, spray the methyl jasmonate solution on the surface of Luo Han Guo every day until the surface drips. Spray once in the morning, in the middle and in the evening, and spray continuously for 10 days.

Embodiment 3

[0032] Step 1, dissolving methyl jasmonate with a volume fraction of 2% ethanol aqueous solution is prepared with a concentration of ethanol mother liquor of 50mmol / L methyl jasmonate, and sterilized with a microporous membrane of 0.22 μm, the jasmine after sterilization Add the ethanol mother liquor of methyl jasmonate to MS solid medium, the amount of methyl jasmonate added is 50μmol / L, sterilize and cool to 24~26℃, wherein, MS solid medium includes: MS, 1.5mg / L L 6-benzylaminoadenine, 0.3mg / L indolebutyric acid, 3.5g / L agar, 30g / L sucrose, and 1.0g / L activated carbon;

[0033] Step 2. Inoculate the Luo Han Guo tissue cultured seedlings in the MS solid medium containing methyl jasmonate obtained in step 1, place them in a relative humidity of 60-66%, light intensity of 1300-1500Lux, light time of 8h / d, temperature of 21~ Culture at 25°C for 28 days;

[0034] Step 3, dissolve methyl jasmonate with a volume fraction of 2% ethanol aqueous solution, prepare a methyl jasmonate s...

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Abstract

The invention discloses a method for increasing the content of siamenoside I in momordica grosvenori. According to the method, jasmonic acid methyl ester is applied in a tissue culture or cultivation process of the momordica grosvenori. By virtue of the method, the yield of the momordica grosvenori siamenoside in the can be effectively increased. The method has the characteristics of fast effectiveness, low cost, simplicity in operation, and convenience in implementation, and the product has no toxicity or residue and the like, can meet the large-scale requirement of the momordica grosvenori siamenoside and has relatively high practicability and promotional values.

Description

technical field [0001] The invention relates to the field of plant biotechnology. More specifically, the present invention relates to a method for increasing the content of simonoside I in Momordica grosvenori. Background technique [0002] Luo Han Guo (Siraitia grosvenorii) is a precious medicinal and sweet plant unique to China. Its fruit is cool in nature and sweet in taste, and has the effects of clearing away heat and moistening lungs, relieving throat and opening sound, laxative and anti-cancer. Studies have shown that Mogroside I is the strongest non-sugar sweetener found in the world so far. It is 563 times sweeter than 5% sucrose at a concentration of 1 / 10,000. ideal sugar substitute. However, simonoside I only exists in the pulp that accounts for less than 15% of the fruit weight, and the content is extremely low, so that the price is expensive, which greatly limits its wide application in food, health products and medicines, and seriously restricts the health o...

Claims

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Application Information

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IPC IPC(8): A01N65/46A01N61/00A01N37/42A01P3/00A01P7/04A01P17/00A01P21/00A01H4/00A01G1/00
CPCA01N65/00A01G22/00A01H4/001A01H4/008A01N37/42A01N61/00A01N65/46A01N2300/00
Inventor 韦荣昌
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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