A method for estimating wild populations of Apis mellifera using drone colonies
A technology for gathering areas and drones, applied in animal husbandry and other directions, can solve the problem of not yet screened out multiple polymorphism-rich loci, etc., and achieve the effect of simple and effective method.
Active Publication Date: 2019-12-20
BEE RES INST CHINESE ACAD OF AGRI SCI
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Problems solved by technology
When analyzing the collected samples, multiple loci with rich polymorphisms have not yet been screened
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Embodiment 1
[0051] (1) DCA positioning
[0052] Through field experiments, it has been found that drone gathering areas are usually distributed in a relatively closed environment. Where there are relatively obvious trees as markers, other trees need to form a semicircular or triangular closed space with them. Screen DCA distribution locations for criteria;
[0053] Fill the balloon with helium, tie the helium-filled balloon with a white nylon rope, tie the transparent fishing line on the nylon rope as a tether, and tie three 10cm-long ropes at intervals on the tether under the balloon. The transparent fishing line is made into a branch shape, and a filter tip is tied at the end of the branch shape, and 20 microliters of queen bee substance (9-ODA) is injected on each filter tip (dissolve 21mg 9-ODA in 1ml isopropanol and 1% ultra-pure Ionized water dilution), put it in the open-air area of the screened DCA distribution area or at the branch with a height of 8-23m, shake the reel, and c...
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The invention provides a method for evaluating the number of wild populations of eastern bees through a drone congregation area. In the method, a drone is firstly induced to determine the congregation area, then a drone sample is acquired in the drone congregation area through adhesion, the DNA of the drone sample is finally extracted and genetically analyzed, the drone is affiliated to the population, and then the number of bee colonies in the specific area coverage is obtained through calculation. The method is simple and convenient in operation steps, efficiently and accurately locates the DCA (Drone Congregation Area) of the eastern bees, and also fills the blank of the existing researches on bees; besides, the method also realizes the effective trapping of the drone; furthermore, the diversity locus of the eastern bees is also systemically analyzed, selected, and genetically analyzed and calculated, then the number of bee colonies in the specific area coverage is effectively determined, and therefore, the method not only fills the blank of the researches on diversity locus of the eastern bees in the prior art, but also effectively measures and calculates the number of the bee colonies in the area.
Description
technical field [0001] The invention relates to the field of population assessment of wild honeybees, in particular to a method for assessing the wild population of Apis orientalis by using drone gathering areas. Background technique [0002] In order to assess changes in the diversity of pollinators and whether pollinators have reached the number required for pollination of plants in a specific area, it is often necessary to count the density of bee colonies, especially wild bee colonies. However, bee colonies in the wild are generally hidden and difficult to find, and data cannot be obtained by directly counting the number of bee colonies. [0003] During the mating season of honeybees, a large number of drones from multiple bee colonies gather together to form Drone Congregation Areas (DCA), where drones mate with virgin queen bees. A large number of drones from the surrounding area gather in the DCA, so that the number of drones in the DCA can reach hundreds to tens of ...
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IPC IPC(8): A01K67/02
CPCA01K67/02
Inventor 丁桂玲黄家兴张红刘彦杰孙成安建东
Owner BEE RES INST CHINESE ACAD OF AGRI SCI