Multi-reagents orderly feeding device for preventing cross contamination

A liquid feeding device and cross-contamination technology, which is applied to feeding devices, laboratory containers, laboratory utensils, etc., can solve the problems of cross-contamination of reagents, inability to effectively eliminate dead volume, inability to rinse clean, etc., to improve The effect of precision

Active Publication Date: 2017-06-13
INST OF SEMICONDUCTORS - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The principle of the fluidic system for the sequential delivery of reagents disclosed in the above-mentioned invention patents is to guide the reagents through the fluidic circuit to the common volume, and flow through other unselected inlets by means of liquid backflush, and then flow to the In the outer ring, there is a waste liquid outlet on one side of the ring; however, the setting of this waste liquid ring cannot effectively eliminate the dead volume, that is, in the actual implementation process, there may be a dead volume area in the waste liquid ring , cannot be rinsed clean, so there is a certain risk of cross-contamination of reagents

Method used

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  • Multi-reagents orderly feeding device for preventing cross contamination
  • Multi-reagents orderly feeding device for preventing cross contamination
  • Multi-reagents orderly feeding device for preventing cross contamination

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Embodiment 1

[0065] ginseng image 3 as shown, image 3 It is a structural schematic diagram of the cleaning process using cleaning liquid in the multi-reagent sequential liquid feeding system provided by the present invention. exist image 3 The multi-reagent sequential liquid feeding system shown is a multi-layer structure, that is, it includes a pipe connection layer, a reagent injection diversion layer, a waste liquid diversion layer, a cleaning liquid diversion layer and a reaction pool connection layer, wherein the pipe connection layer The multi-reagent inlets 2101-2110 are respectively connected with the reagent inflow holes 2201-2210 in the reagent injection diversion layer through radial pipes; The hole 2311 and the hole 2411 in the cleaning fluid diversion layer are connected through radial pipes; the waste liquid outlet 2112, the reagent injection hole 2212 in the diversion layer, and the node hole 2312 in the waste liquid diversion layer are connected through radial pipes. ...

Embodiment 2

[0069] ginseng Figure 4 as shown, Figure 4It is a schematic structural diagram of the reagent feeding and cleaning process of the multi-reagent sequential liquid feeding system provided by the present invention. The principle of the reagent feeding and cleaning process is that the reagents start to be fed, but they do not enter the reaction pool immediately, but cooperate with the discharge system through the cleaning process of the cleaning solution to discharge the reagents that may be contaminated at the reagent inlet. In this embodiment, there are 10 reagent inlets. The process will be described by taking 2109 port as an example to start liquid feeding. Other reagent ports 2101-2108 and 2110 stop liquid feeding, and the cleaning reagent continues the cleaning process. The reagent flows into the channel connecting the hole 2220 and the hole 2224 from the hole 2209, and then as the cleaning liquid flows into the 23rd layer of the waste liquid diversion layer, all the reag...

Embodiment 3

[0071] ginseng Figure 5 as shown, Figure 5 It is a structural schematic diagram of the process of injecting reagents into the reaction pool by the multi-reagent sequential liquid feeding system provided by the present invention. The process of injecting the reagent into the reaction pool is after the previous process, that is, the reagent is put into the cleaning process, and the injection of the cleaning solution is stopped. The reagent enters the 22nd floor through the hole 2109 and the hole 2209. On the 22nd floor plane, the reagent flows from the hole 2209 into the connecting hole 2220 and the node. In the channel of the hole 2224, a part flows to the direction of the hole 2220, and the other part flows to the direction of the node hole 2224; the reagent flowing into the direction of the node hole 2224 will be divided into multiple directions, and some of them will leave the 22nd layer and pass through the direction perpendicular to the 22nd layer. Node holes 2224, hole...

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Abstract

The invention provides a multi-reagents orderly feeding device for preventing cross contamination. The device of the invention comprises a housing and a multi-reagents orderly feeding system, which is arranged inside the housing. The multi-reagents orderly feeding system is a multi-layered structure and at least comprises a pipe connection layer, a reagent injection division layer, a waste liquid diversion layer, a cleaning fluid diversion layer and a reaction tank connection layer. The pipe connection layer, the reagent injection diversion layer, the waste liquid diversion layer, the cleaning fluid diversion layer and the reaction tank connection layer are successively connected through radial pipelines. The multi-reagents orderly feeding device has no dead volume and also effectively avoids reagent residues and cross contamination during feeding of multiple reagents.

Description

technical field [0001] The invention relates to the field of liquid flow control, in particular to a multi-reagent sequential liquid feeding device for preventing cross-contamination. Background technique [0002] In the field of liquid flow control, especially in biochemical reaction systems, the sequential feeding of multiple reagents is a common liquid flow control step, but effectively avoiding cross-contamination of multiple reagents in the liquid flow control step is currently the most difficult solved problem. [0003] U.S. Patent US09149803 and its continuation application patent CN102802402 in China provide a fluidic control system for sequential delivery of reagents, wherein the fluidic circuit for controlling multiple fluids includes a fluidic control node with an outlet, multiple fluidic an inlet, at least one waste port, and a plurality of channels each providing a different path for fluid communication between the at least one waste port and the fluidic juncti...

Claims

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Application Information

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IPC IPC(8): B01J4/00B01L3/00
CPCB01J4/001B01J4/008B01J2204/002B01L3/5027B01L2300/0867B01L2300/0887
Inventor 魏清泉俞育德刘文文刘元杰杨翎
Owner INST OF SEMICONDUCTORS - CHINESE ACAD OF SCI
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