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A method for large-scale and efficient cultivation of Synechococcus sp. 7002

A high-efficiency technology for Synechococcus, which is applied in the field of large-scale and high-efficiency cultivation of Synechococcus 7002, can solve the problems of being easily affected by bacterial contamination, unable to achieve higher density, and high cultivation costs, and achieve economical convenience, easy control, and cultivation. Efficient effect

Active Publication Date: 2020-04-03
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] 1) Unlike microalgae such as spirulina and chlorella, Synechococcus 7002 is highly sensitive to external bacterial contamination and is easily affected by bacterial contamination; 2) Synechococcus 7002 at different growth stages has different requirements for culture conditions , inappropriate light and carbon supply can easily inhibit its growth; 3) Although the division speed is fast, the efficiency of large-scale culture is low, and a high density cannot be achieved in a short period of time, resulting in high culture costs

Method used

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  • A method for large-scale and efficient cultivation of Synechococcus sp. 7002
  • A method for large-scale and efficient cultivation of Synechococcus sp. 7002

Examples

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Embodiment 1

[0027] A method for large-scale and efficient cultivation of Synechococcus 7002 includes the following steps (the cultivation process is as figure 2 Shown):

[0028] (1) First, inoculate the seed solution, the initial inoculation density value OD 750 0.10, the culture volume of each culture bag is 15L, the vitamin B in the medium 12 The concentration was increased to 10 µg / L, and the light intensity was controlled to 20 µmol / (m 2 •S), the carbon dioxide concentration is 0.5%, and the ventilation rate is 45 L / h;

[0029] (2) Secondly, monitor the growth of Synechococcus 7002 in real time, when its density reaches OD 750 When it is 0.4, adjust the light intensity to 40 µmol / (m 2 •S), the carbon dioxide concentration is 0.7%, and the ventilation rate is 60 L / h;

[0030] (3) When the density of Synechococcus 7002 reaches OD 750 When it is 1.0, adjust the light intensity to 80 µmol / (m 2 •S), the concentration of carbon dioxide is 0.8%, and the ventilation rate is 90 L / h. At this time, i...

Embodiment 2

[0037] A method for large-scale and efficient cultivation of Synechococcus 7002 includes the following steps (the cultivation process is as figure 2 Shown):

[0038] (1) First, inoculate the seed solution, the initial inoculation density value OD 750 0.12, the culture volume of each culture bag is 18L, the vitamin B in the medium 12 The concentration was increased to 20 µg / L, and the light intensity was controlled to 25 µmol / (m 2 •S), the carbon dioxide concentration is 0.5%, and the ventilation rate is 55 L / h;

[0039] (2) Second, monitor the growth of Synechococcus 7002 in real time, and when its density reaches OD 750 When it is 0.5, adjust the light intensity to 50 µmol / (m 2 •S), the carbon dioxide concentration is 0.7%, and the ventilation rate is 70 L / h;

[0040] (3) When the density of Synechococcus 7002 reaches OD 750 When it is 1.2, adjust the light intensity to 100 µmol / (m 2 •S), the concentration of carbon dioxide is 0.9%, and the ventilation rate is 105 L / h. At this tim...

Embodiment 3

[0047] A method for large-scale and efficient cultivation of Synechococcus 7002 includes the following steps (the cultivation process is as figure 2 Shown):

[0048] (1) First, inoculate the seed solution, the initial inoculation density value OD 750 0.15, the culture volume of each culture bag is 20L, and the vitamin B in the medium 12 The concentration was increased to 40 µg / L, and the light intensity was controlled to 30 µmol / (m 2 •S), the carbon dioxide concentration is 0.6%, and the ventilation rate is 60 L / h;

[0049] (2) Second, monitor the growth of Synechococcus 7002 in real time, and when its density reaches OD 750 When it is 0.6, adjust the light intensity to 60 µmol / (m 2 •S), the carbon dioxide concentration is 0.8%, and the ventilation rate is 80 L / h;

[0050] (3) When the density of Synechococcus 7002 reaches OD 750 When it is 1.5, adjust the light intensity to 120 µmol / (m 2 •S), the concentration of carbon dioxide is 1.0%, and the ventilation rate is 120 L / h. At this...

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Abstract

The invention discloses a method for efficiently culturing synechococcus 7002 on a large scale. A suspended culture bag culture mode is adopted, the illumination intensity, the CO2 concentration and the ventilator capacity are regulated step by step according to the growth state of the synechococcus 7002, and vitamin B12 and iron ions are added to promote growth. The method is applicable to indoor or outdoor culture of the synechococcus. Obviously, the synechococcus 7002 can be efficiently fermented on a large scale, the efficiency is fully improved, the cost is saved, and the method is an effective method for producing synechococcus 7002 algae powder and added fermentation products. A culture bag is adopted for culture, can provide a relatively closed culture space, has the better culture effect, is economical and convenient, facilitates condition control and can be reused.

Description

Technical field [0001] The invention belongs to the field of microalgae cultivation, and specifically relates to a method for large-scale and high-efficiency cultivation of Synechococcus 7002. Background technique [0002] Microalgae is considered to be the most promising raw material for sustainable production of food, feed, chemical materials and fuels. Microalgae have high nutritional value and are rich in pigments, proteins, lipids, and various mineral elements and vitamins. Synechococcus is one of the two most important cyanobacteria in the ocean. It is a dominant component of marine phytoplankton communities. It contributes 16.7% to marine primary productivity. It plays a role in the photosynthesis, carbon cycle and food chain of marine ecosystems. A pivotal role. Synechococcus 7002 is an offshore model microalgae, and it is also the fastest dividing cyanobacteria found so far. It has a natural exogenous DNA transformation system and its entire genome has been deciphered....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 曾名湧高风正吴浩浩黄敏冯广鑫
Owner OCEAN UNIV OF CHINA