Blood cell stabilizer and application thereof

A technology of blood cells and stabilizers, applied in the field of cell protection agents, can solve the problems of cell death, lack of protective effect on cells, failure to maintain surface antigens of blood cells, etc., and achieve the effect of maintaining physical properties

Inactive Publication Date: 2017-06-20
ZHUHAI LIVZON CYNVENIO DIAGNOSTICS
View PDF0 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, conventional blood anticoagulants like EDTA have no protective effect on the cells in the blood: the cells in th

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Blood cell stabilizer and application thereof
  • Blood cell stabilizer and application thereof
  • Blood cell stabilizer and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A blood cell stabilizer, which includes liquid A and liquid B, liquid A is a preservation liquid for maintaining the physical properties of cells, and liquid B is a fixative liquid for fixing cells; wherein, liquid A includes the following components in mass percentage : EDTA 5%, sodium fluoride 5%, 4-(2-aminoethyl) benzenesulfonyl fluoride 0.5%, water 89.5%; B liquid includes the following components in mass percentage: formaldehyde polymer 2.5%, sodium hydroxide Solution 2.5%, hydrochloric acid 2.5%, water 92.5%.

Embodiment 2

[0032] A blood cell stabilizer, which includes liquid A and liquid B, liquid A is a preservation liquid for maintaining the physical properties of cells, and liquid B is a fixative liquid for fixing cells; wherein, liquid A includes the following components in mass percentage : 7% citrate, 4% sodium orthosulfate, 0.7% phenylmethylsulfonyl fluoride, 88.3% water; B liquid includes the following components in mass percentage: acetaldehyde polymer 3%, potassium hydroxide solution 4% , hydrochloric acid 2%, water 91%.

Embodiment 3

[0034] A blood cell stabilizer, which includes liquid A and liquid B, liquid A is a preservation liquid for maintaining the physical properties of cells, and liquid B is a fixative liquid for fixing cells; wherein, liquid A includes the following components in mass percentage : Potassium fluoride 4%, sodium glycerophosphate 6%, leupeptin 1%, water 89%; liquid B includes the following components in mass percentage: propionaldehyde polymer 2%, sodium hydroxide solution 5%, hydrochloric acid 1% , Water 92%.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Osmotic pressureaaaaaaaaaa
Login to view more

Abstract

The invention discloses a blood cell stabilizer and application thereof, belonging to the technical field of cell protection agents. The blood cell stabilizer includes liquid A and liquid B, wherein liquid A is a preservation solution for maintaining the physical properties of cells, and liquid B is a fixative solution for fixing cells; wherein, liquid A includes the following components in mass percentage: anticoagulant Agent 1-10%, phosphatase inhibitor 1-10%, protease inhibitor 0.005-1%, water balance; B liquid includes the following components in mass percentage: aldehyde polymer 0.1-5%, alkaline salt 0.1 ~5%, hydrochloric acid 0.1~5%, water balance. The blood cell stabilizer enables the blood components and cells in the blood to be preserved for more than 192 hours without low temperature treatment. The processed blood samples will not affect the subsequent molecular detection of the samples, and can be widely used in the immune analysis of cell components in blood samples , Nucleic acid analysis or protein analysis related experiments.

Description

technical field [0001] The invention relates to the technical field of cell protection agents, in particular to a blood cell stabilizer capable of maintaining the morphology of blood components, the surface antigen characteristics of cells in blood, the integrity of cell nuclei and the stability of nucleic acids and its application. Background technique [0002] When the whole blood sample is collected, due to changes in the temperature, pH and oxygen mass percentage of the peripheral environment, the platelets will release procoagulant factors after the blood leaves the human body, causing blood coagulation, and at the same time, the blood cells will also lose cell compactness due to environmental changes , and thus die. During this process, the physicochemical properties and biomarkers (including proteins, nucleic acids, small molecules, etc.) of various components of plasma and cellular components in blood will also change, thereby affecting the diagnostic results of bloo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N15/10G01N33/49G01N1/30
CPCG01N15/10G01N1/30G01N33/491G01N2015/1006
Inventor 蒋世强周燕玲范献军
Owner ZHUHAI LIVZON CYNVENIO DIAGNOSTICS
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap