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Identification method of animal origin and source, establishing method of identification model, and applications of establishing method

An identification method and a technology for establishing a method, which are applied in the field of identification of animal origin and can solve problems such as difficulty in identification of water sources.

Inactive Publication Date: 2017-06-23
深圳华大优选科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, it is only possible to identify whether the hairy crab is a Chinese mitten crab through genetic testing, but there are great difficulties in identifying the source of its water system.

Method used

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  • Identification method of animal origin and source, establishing method of identification model, and applications of establishing method
  • Identification method of animal origin and source, establishing method of identification model, and applications of establishing method
  • Identification method of animal origin and source, establishing method of identification model, and applications of establishing method

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Experimental program
Comparison scheme
Effect test

Embodiment

[0031]Embodiment, determination of intestinal microorganisms of hairy crab

[0032] The determination of intestinal microorganisms of hairy crabs was carried out according to the following steps:

[0033] 1. Extraction of intestinal microbial DNA

[0034] Collect 6 Yangcheng Lake hairy crabs and 4 Taihu Lake hairy crabs and mark them, and collect intestinal microbial samples from these 10 hairy crabs and place them in 2ml EP tubes.

[0035] The sample information is shown in Table 1:

[0036] Table 1: Samples

[0037] Sample source sample name Yangcheng Lake hairy crabs 1A, 2A, 3A, 4A, 5A, 6A Taihu hairy crab 7A, 8A, 9A, 10A

[0038] DNA extraction was carried out according to the guidance in "Gut Microbial DNA Extraction Method".

[0039] 1) Add cell lysate to the EP tube containing the sample to be extracted. Add 500ul glass beads with a diameter of 0.1mm at the same time,

[0040] 2) The sample tube is placed in a high-throughput tissue d...

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Abstract

The invention discloses an identification method of the animal origin and source, an establishing method of a model, and applications of the establishing method. The identification method comprises the following steps: acquiring abundance information of animal enteric microorganisms; and carrying out correlation analysis on the abundance information, and judging the origin and source of animals according to the analysis result. The invention further provides an establishing method of the identification model for the animal origin and source and applications of the establishing method. With the identification method of the animal origin and source and the establishing method of the identification model for the animal origin and source provided by the invention, the animal origin and source can be identified.

Description

technical field [0001] The present invention relates to a method for identifying the source of animal origin, a method for establishing an identification model and its application, in particular to a method for identifying the source of origin through animal intestinal microbes, a method for establishing an identification model through animal intestinal microbes and its application. Background technique [0002] The identification of the source of origin involves the identification of animal species and commodities with the brand value of the origin. The method of using intestinal microorganisms and the establishment of identification models can efficiently identify the origin of animals. [0003] Take hairy crabs as an example: hairy crabs are people's favorite delicacy. Due to the brand competitiveness of Yangcheng Lake hairy crabs, many other brands of hairy crabs carry out commercial activities under the banner of Yangcheng Lake hairy crabs. According to statistics, the ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12Q1/10C12Q1/04
CPCC12Q1/689C12Q2600/158C12Q2600/178
Inventor 刘楚新李飞达肖丽萍刘传李晓平刘欢徐迅
Owner 深圳华大优选科技有限公司
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