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Culture medium suitable for growth of Ustilaginoidea virens

A rice smut fungus and a culture medium technology, which is applied in the field of rice smut fungus culture medium, can solve the problems of slow growth of rice smut fungus, low efficiency, inconvenient scientific investigation and control research of rice smut fungus, etc., and is suitable for large-scale Production and use, shortening time, good use cost and economic effect

Active Publication Date: 2017-06-30
ZHEJIANG UNIV
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Problems solved by technology

[0006] According to the research survey, most of the rice smut culture medium used in the research related to the cultivation of rice smut bacteria are commercial mediums on the market, such as potato sucrose agar medium, potato dextrose agar medium, Wakimoto Tetsu's medium and rice isolated medium. Culture medium, the specific situation of culture is that when cultivating for 30 days, the mycelia growth of rice smut is as follows: PDA medium: 60.3mm; PSA medium: 69.8mm; Wakimoto Tetsu's medium: 59.6mm; rice grain medium: 65.9mm, rice smut bacteria grow slowly on these media, which brings great inconvenience to the scientific investigation and control research of rice smut bacteria, and low efficiency is a threshold for research on rice smut bacteria

Method used

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  • Culture medium suitable for growth of Ustilaginoidea virens
  • Culture medium suitable for growth of Ustilaginoidea virens
  • Culture medium suitable for growth of Ustilaginoidea virens

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Embodiment 1

[0027] Embodiment 1, a kind of substratum P.S.G that is suitable for the growth of rice false smut bacteria, this substratum main body is made up of the composition of following weight content (%): sucrose 1%, peptone 2%, gellan gum 0.3%, Mg 2+ 0.02%, the balance is water (ultrapure water); Mg 2+ for magnesium sulfate.

[0028] Add the following inorganic salts to the main body of the medium: 0.0025% potassium hydrogen phosphate, 0.0125% sodium chloride, 0.0005% ferrous sulfate, 0.0005% sodium molybdate, 0.0005% manganese sulfate, and 0.01% calcium carbonate.

[0029] That is, 25 mg of potassium hydrogen phosphate, 125 mg of sodium chloride, 5 mg of ferrous sulfate, 5 mg of sodium molybdate, 5 mg of manganese sulfate, and 100 mg of calcium carbonate were added to the main body of 1 kg of medium to obtain a medium suitable for the growth of rice smut bacteria.

[0030] The medium P.S.G is the same as the conventional medium, and needs to be subjected to routine high-temperatur...

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Abstract

The invention discloses a culture medium suitable for growth of Ustilaginoidea virens. The main body of the culture medium comprises, by weight, 0.1 to 5% of cane sugar, 0.1 to 5% of peptone, 0.1 to 1% of gellan gum and 0.02 to 0.1% of Mg<2+>, with the balance being water. Trace amounts of inorganic salts consisting of potassium hydrogen diphosphate, sodium chloride, ferrous sulfate, sodium molybdate, manganese sulfate and calcium carbonate are added into the main body of the culture medium so as to obtain the culture medium suitable for growth of Ustilaginoidea virens.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a medium suitable for cultivating rice smut bacteria. Background technique [0002] Claviceps oryzae-sativae Hashioka belongs to Ascomycota phylum Ergotaceae, and its anamorphic form is Ustilaginoidea virens (Cooke) Takahashi, synonym: U.oryzae (Pat) Bref. Fungus, mainly infects the fungal diseases caused by rice ears. Pathological studies found that U.virens invaded from the space between the lemma and palea of ​​the spikelet, forming a rice ball that is larger than the grain and covered with a large number of yellow or dark green chlamydospores. The center of the diseased grain is densely composed of hyphae. The white fleshy block, its periphery can be divided into three layers due to the different maturity of mycelium producing chlamydospores: the outer layer is the earliest mature, dark green or olive green; the second layer is orange-yellow; the third layer is yellow. Rice fal...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 王蒙岑刘晓玉杜永均钱圆朱国念
Owner ZHEJIANG UNIV
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